Search results for the GEO ID: GSE40368  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM992333 | GPL1261 |
|
RProia1_1_LA_Mouse430_2_1_1WT-und
|
01-31-12_RProia1_1_LA_Mouse430_2_1_1WT-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1+/+
|
|
| Sample_geo_accession | GSM992333
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992333/suppl/GSM992333_01-31-12_RProia1_1_LA_Mouse430_2_1_1WT-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992333/suppl/GSM992333_01-31-12_RProia1_1_LA_Mouse430_2_1_1WT-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992334 | GPL1261 |
|
RProia10_10_LA_Mouse430_2_10_10KO-und
|
01-31-12_RProia10_10_LA_Mouse430_2_10_10KO-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1-/-
|
|
| Sample_geo_accession | GSM992334
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992334/suppl/GSM992334_01-31-12_RProia10_10_LA_Mouse430_2_10_10KO-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992334/suppl/GSM992334_01-31-12_RProia10_10_LA_Mouse430_2_10_10KO-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992335 | GPL1261 |
|
RProia2_2_LA_Mouse430_2_2_2WT-und
|
01-31-12_RProia2_2_LA_Mouse430_2_2_2WT-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1+/+
|
|
| Sample_geo_accession | GSM992335
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992335/suppl/GSM992335_01-31-12_RProia2_2_LA_Mouse430_2_2_2WT-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992335/suppl/GSM992335_01-31-12_RProia2_2_LA_Mouse430_2_2_2WT-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992336 | GPL1261 |
|
RProia3_3_LA_Mouse430_2_3_3WT-und
|
01-31-12_RProia3_3_LA_Mouse430_2_3_3WT-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1+/+
|
|
| Sample_geo_accession | GSM992336
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992336/suppl/GSM992336_01-31-12_RProia3_3_LA_Mouse430_2_3_3WT-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992336/suppl/GSM992336_01-31-12_RProia3_3_LA_Mouse430_2_3_3WT-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992337 | GPL1261 |
|
RProia4_4_LA_Mouse430_2_4_4WT-und
|
01-31-12_RProia4_4_LA_Mouse430_2_4_4WT-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1+/+
|
|
| Sample_geo_accession | GSM992337
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992337/suppl/GSM992337_01-31-12_RProia4_4_LA_Mouse430_2_4_4WT-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992337/suppl/GSM992337_01-31-12_RProia4_4_LA_Mouse430_2_4_4WT-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992338 | GPL1261 |
|
RProia5_5_LA_Mouse430_2_5_5WT-und
|
01-31-12_RProia5_5_LA_Mouse430_2_5_5WT-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1+/+
|
|
| Sample_geo_accession | GSM992338
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992338/suppl/GSM992338_01-31-12_RProia5_5_LA_Mouse430_2_5_5WT-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992338/suppl/GSM992338_01-31-12_RProia5_5_LA_Mouse430_2_5_5WT-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992339 | GPL1261 |
|
RProia6_21_LA_Mouse430_2_6_21KO-und
|
01-31-12_RProia6_21_LA_Mouse430_2_6_21KO-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1-/-
|
|
| Sample_geo_accession | GSM992339
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992339/suppl/GSM992339_01-31-12_RProia6_21_LA_Mouse430_2_6_21KO-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992339/suppl/GSM992339_01-31-12_RProia6_21_LA_Mouse430_2_6_21KO-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992340 | GPL1261 |
|
RProia7_23_LA_Mouse430_2_7_23KO-und
|
01-31-12_RProia7_23_LA_Mouse430_2_7_23KO-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1-/-
|
|
| Sample_geo_accession | GSM992340
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992340/suppl/GSM992340_01-31-12_RProia7_23_LA_Mouse430_2_7_23KO-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992340/suppl/GSM992340_01-31-12_RProia7_23_LA_Mouse430_2_7_23KO-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
| | |
|
GSM992341 | GPL1261 |
|
RProia8_8_LA_Mouse430_2_8_8KO-und
|
01-31-12_RProia8_8_LA_Mouse430_2_8_8KO-und
|
tissue: Primary keratinocytes
genotype/variaion: Sgpp1-/-
|
|
| Sample_geo_accession | GSM992341
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992341/suppl/GSM992341_01-31-12_RProia8_8_LA_Mouse430_2_8_8KO-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992341/suppl/GSM992341_01-31-12_RProia8_8_LA_Mouse430_2_8_8KO-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
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GSM992342 | GPL1261 |
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RProia9_9_LA_Mouse430_2_9_9KO-und
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01-31-12_RProia9_9_LA_Mouse430_2_9_9KO-und
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tissue: Primary keratinocytes
genotype/variaion: Sgpp1-/-
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| Sample_geo_accession | GSM992342
| | Sample_status | Public on Aug 09 2013
| | Sample_submission_date | Aug 24 2012
| | Sample_last_update_date | Aug 09 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | RNA was isolated samples from KO and WT genotype
| | Sample_growth_protocol_ch1 | Mouse were collected from NIDDK animal core facility. Primary keratinocytes from the skin of Sgpp1+/+ and Sgpp1-/- mice were grown in 0.05mM Ca2+-containing EMEM medium plus 8% fetal calf serum (low Ca2+ medium) for 3-4 days and harvested for isolation of mRNA.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | RMA nomanization method of software Partek6.6
| | Sample_platform_id | GPL1261
| | Sample_contact_name | WeiPing,,Chen
| | Sample_contact_email | weipingChen@niddk.nih.gov
| | Sample_contact_phone | 301-496-0175
| | Sample_contact_laboratory | Microarray Core Lab
| | Sample_contact_department | MCL
| | Sample_contact_institute | NIDDK/NIH
| | Sample_contact_address | Bldg 8, Room 1A11, NIDDK/NIH
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992342/suppl/GSM992342_01-31-12_RProia9_9_LA_Mouse430_2_9_9KO-und.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992342/suppl/GSM992342_01-31-12_RProia9_9_LA_Mouse430_2_9_9KO-und.CHP.gz
| | Sample_series_id | GSE40368
| | Sample_data_row_count | 45101
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