Result

Search results for the GEO ID: GSE40381

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM992577

GPL1355

pLV-GFP-treated HSCs 1

quiescent hepatic stellate cells

strain: Sprague-Dawley age: adult gender: male infection: pLV-GFP

HSCs are derived from the liver of adult male Sprague-Dawley rats Gene expression data from activated HSCs treated by pLV-GFP

Sample_geo_accession	GSM992577
Sample_status	Public on Aug 28 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Aug 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
Sample_growth_protocol_ch1	HSCs were cultured in cultured in Dulbecco’s modified Eagle medium (DMEM) containing 5 % fetal calf serum, penicillin (100 IU/ ml) and st reptomycin (100 mg/ ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Sample_hyb_protocol	Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Sample_scan_protocol	Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software (Affymetrix, Santa Clara, CA, US) with default settings.
Sample_data_processing	Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software (Agilent technologies, Santa Clara, CA, US).
Sample_platform_id	GPL1355
Sample_contact_name	Qin,,Pan
Sample_contact_email	pan_qin@yeah.net
Sample_contact_institute	Xin Hua hospital
Sample_contact_address	1665 Kongjiang Rd.
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200092
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992577/suppl/GSM992577_pLV-GFP-treated_HSCs_1.CEL.gz
Sample_series_id	GSE40381
Sample_data_row_count	31099

GSM992578

GPL1355

pLV-GFP-treated HSCs 2

quiescent hepatic stellate cells

strain: Sprague-Dawley age: adult gender: male infection: pLV-GFP

HSCs are derived from the liver of adult male Sprague-Dawley rats Gene expression data from activated HSCs treated by pLV-GFP

Sample_geo_accession	GSM992578
Sample_status	Public on Aug 28 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Aug 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
Sample_growth_protocol_ch1	HSCs were cultured in cultured in Dulbecco’s modified Eagle medium (DMEM) containing 5 % fetal calf serum, penicillin (100 IU/ ml) and st reptomycin (100 mg/ ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Sample_hyb_protocol	Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Sample_scan_protocol	Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software (Affymetrix, Santa Clara, CA, US) with default settings.
Sample_data_processing	Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software (Agilent technologies, Santa Clara, CA, US).
Sample_platform_id	GPL1355
Sample_contact_name	Qin,,Pan
Sample_contact_email	pan_qin@yeah.net
Sample_contact_institute	Xin Hua hospital
Sample_contact_address	1665 Kongjiang Rd.
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200092
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992578/suppl/GSM992578_pLV-GFP-treated_HSCs_2.CEL.gz
Sample_series_id	GSE40381
Sample_data_row_count	31099

GSM992579

GPL1355

pLV-GFP-treated HSCs 3

quiescent hepatic stellate cells

strain: Sprague-Dawley age: adult gender: male infection: pLV-GFP

HSCs are derived from the liver of adult male Sprague-Dawley rats Gene expression data from activated HSCs treated by pLV-GFP

Sample_geo_accession	GSM992579
Sample_status	Public on Aug 28 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Aug 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
Sample_growth_protocol_ch1	HSCs were cultured in cultured in Dulbecco’s modified Eagle medium (DMEM) containing 5 % fetal calf serum, penicillin (100 IU/ ml) and st reptomycin (100 mg/ ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Sample_hyb_protocol	Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Sample_scan_protocol	Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software (Affymetrix, Santa Clara, CA, US) with default settings.
Sample_data_processing	Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software (Agilent technologies, Santa Clara, CA, US).
Sample_platform_id	GPL1355
Sample_contact_name	Qin,,Pan
Sample_contact_email	pan_qin@yeah.net
Sample_contact_institute	Xin Hua hospital
Sample_contact_address	1665 Kongjiang Rd.
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200092
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992579/suppl/GSM992579_pLV-GFP-treated_HSCs_3.CEL.gz
Sample_series_id	GSE40381
Sample_data_row_count	31099

GSM992580

GPL1355

pLV-miR-16-treated HSCs 1

quiescent hepatic stellate cells

strain: Sprague-Dawley age: adult gender: male infection: pLV-miR-16

HSCs are derived from the liver of adult male Sprague-Dawley rats Gene expression data from activated HSCs treated by pLV-miR-16

Sample_geo_accession	GSM992580
Sample_status	Public on Aug 28 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Aug 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
Sample_growth_protocol_ch1	HSCs were cultured in cultured in Dulbecco’s modified Eagle medium (DMEM) containing 5 % fetal calf serum, penicillin (100 IU/ ml) and st reptomycin (100 mg/ ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Sample_hyb_protocol	Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Sample_scan_protocol	Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software (Affymetrix, Santa Clara, CA, US) with default settings.
Sample_data_processing	Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software (Agilent technologies, Santa Clara, CA, US).
Sample_platform_id	GPL1355
Sample_contact_name	Qin,,Pan
Sample_contact_email	pan_qin@yeah.net
Sample_contact_institute	Xin Hua hospital
Sample_contact_address	1665 Kongjiang Rd.
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200092
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992580/suppl/GSM992580_pLV-miR-16-treated_HSCs_1.CEL.gz
Sample_series_id	GSE40381
Sample_data_row_count	31099

GSM992581

GPL1355

pLV-miR-16-treated HSCs 2

quiescent hepatic stellate cells

strain: Sprague-Dawley age: adult gender: male infection: pLV-miR-16

HSCs are derived from the liver of adult male Sprague-Dawley rats Gene expression data from activated HSCs treated by pLV-miR-16

Sample_geo_accession	GSM992581
Sample_status	Public on Aug 28 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Aug 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
Sample_growth_protocol_ch1	HSCs were cultured in cultured in Dulbecco’s modified Eagle medium (DMEM) containing 5 % fetal calf serum, penicillin (100 IU/ ml) and st reptomycin (100 mg/ ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Sample_hyb_protocol	Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Sample_scan_protocol	Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software (Affymetrix, Santa Clara, CA, US) with default settings.
Sample_data_processing	Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software (Agilent technologies, Santa Clara, CA, US).
Sample_platform_id	GPL1355
Sample_contact_name	Qin,,Pan
Sample_contact_email	pan_qin@yeah.net
Sample_contact_institute	Xin Hua hospital
Sample_contact_address	1665 Kongjiang Rd.
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200092
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992581/suppl/GSM992581_pLV-miR-16-treated_HSCs_2.CEL.gz
Sample_series_id	GSE40381
Sample_data_row_count	31099

GSM992582

GPL1355

pLV-miR-16-treated HSCs 3

quiescent hepatic stellate cells

strain: Sprague-Dawley age: adult gender: male infection: pLV-miR-16

HSCs are derived from the liver of adult male Sprague-Dawley rats Gene expression data from activated HSCs treated by pLV-miR-16

Sample_geo_accession	GSM992582
Sample_status	Public on Aug 28 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Aug 28 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
Sample_growth_protocol_ch1	HSCs were cultured in cultured in Dulbecco’s modified Eagle medium (DMEM) containing 5 % fetal calf serum, penicillin (100 IU/ ml) and st reptomycin (100 mg/ ml).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Total RNA were amplified, labeled and purified by using GeneChip 3’IVT Express Kit (Cat#901229, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions to obtain biotin labeled cRNA.
Sample_hyb_protocol	Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix, Santa Clara, CA, US)in Hybridization Oven 645 (Cat#00-0331-220V, Affymetrix, Santa Clara, CA, US)and Fluidics Station 450 (Cat#00-0079, Affymetrix, Santa Clara, CA, US) followed the manufacturer’s instructions.
Sample_scan_protocol	Slides were scanned by GeneChip® Scanner 3000 (Cat#00-00212, Affymetrix, Santa Clara, CA, US) and Command Console Software (Affymetrix, Santa Clara, CA, US) with default settings.
Sample_data_processing	Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software (Agilent technologies, Santa Clara, CA, US).
Sample_platform_id	GPL1355
Sample_contact_name	Qin,,Pan
Sample_contact_email	pan_qin@yeah.net
Sample_contact_institute	Xin Hua hospital
Sample_contact_address	1665 Kongjiang Rd.
Sample_contact_city	Shanghai
Sample_contact_zip/postal_code	200092
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992582/suppl/GSM992582_pLV-miR-16-treated_HSCs_3.CEL.gz
Sample_series_id	GSE40381
Sample_data_row_count	31099

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