Result

Search results for the GEO ID: GSE40400

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Description

Characteristics

GSM992854

GPL570

Cumulus cells from oocyte at MII stage , biological rep1

Cumulus cells isolated from oocyte at stage metaphase II (MII) of PCOS patients

cell type: Cumulus cells cell cyle: metaphase II disease state: Polycystic ovary syndrome

Gene expression data from cumulus cells isolated from oocyte at MII stage

Sample_geo_accession	GSM992854
Sample_status	Public on Jun 04 2013
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Jun 04 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	All the PCOS participants included in the study were women undergoing in vitro fertilization.Ovarian stimulation and oocyte retrieval protocols were carried out as below.pituitary down-regulation was started with mid-luteal phase GnRH agonist (Diphereline 3.75mg, PharmaBiotech, Paris, France). Once adequate pituitary down-regulation was confirmed [absence of ovarian cysts>=18mm in diameter and serum estradiol (E2) levels of <50 pg/ml]. Controlled ovarian stimulation was conducted with recombinant FSH (Follitropin Alfa, Serono, London, UK). Human chorionic gonadotropin (hCG; 10,000 IU, Profasi; Serono Laboratories) was given in when two or more follicles were at least 18mm in diameter and the serum E2 levels were at least 300pg/ml per dominant follicle. Oocyte retrieval was performed 36h after hCG administration. Cumulus cells were separated from the oocyte with strippers mechanically and washed twice in cold phosphate buffer saline (Dubelcco’s medium) (Gibco, invitrogen, Paris, France) then centrifuged at 200g for 10 min. The supernatant was removed and the pellet was resuspended in RLT buffer of the Reasy Mini Kit.
Sample_growth_protocol_ch1	All patients with PCOS fulfilled the 1990 National Institutes of Health and the revised 2003 Rotterdam consensus diagnostic criteria for PCOS, and the patients which average age was 32 years (range 25-38 years) were enrolled in the study.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 to analyze the hybridization data.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Xin,,Huang
Sample_contact_email	huangxin92129@gmail.com
Sample_contact_phone	86-535-6691999-83911
Sample_contact_laboratory	Embryo Culture Lab
Sample_contact_department	Reproduction Medical Centre
Sample_contact_institute	Yuhuangding hospital of Yantai
Sample_contact_address	Yuhuangding east street 20
Sample_contact_city	Yan tai
Sample_contact_state	Shan dong
Sample_contact_zip/postal_code	264000
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992854/suppl/GSM992854_CCMII1.CEL.gz
Sample_series_id	GSE40400
Sample_data_row_count	54613

GSM992855

GPL570

Cumulus cells from oocyte at MII stage , biological rep2

Cumulus cells isolated from oocyte at stage metaphase II (MII) of PCOS patients

cell type: Cumulus cells cell cyle: metaphase II disease state: Polycystic ovary syndrome

Gene expression data from cumulus cells isolated from oocyte at MII stage

Sample_geo_accession	GSM992855
Sample_status	Public on Jun 04 2013
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Jun 04 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	All the PCOS participants included in the study were women undergoing in vitro fertilization.Ovarian stimulation and oocyte retrieval protocols were carried out as below.pituitary down-regulation was started with mid-luteal phase GnRH agonist (Diphereline 3.75mg, PharmaBiotech, Paris, France). Once adequate pituitary down-regulation was confirmed [absence of ovarian cysts>=18mm in diameter and serum estradiol (E2) levels of <50 pg/ml]. Controlled ovarian stimulation was conducted with recombinant FSH (Follitropin Alfa, Serono, London, UK). Human chorionic gonadotropin (hCG; 10,000 IU, Profasi; Serono Laboratories) was given in when two or more follicles were at least 18mm in diameter and the serum E2 levels were at least 300pg/ml per dominant follicle. Oocyte retrieval was performed 36h after hCG administration. Cumulus cells were separated from the oocyte with strippers mechanically and washed twice in cold phosphate buffer saline (Dubelcco’s medium) (Gibco, invitrogen, Paris, France) then centrifuged at 200g for 10 min. The supernatant was removed and the pellet was resuspended in RLT buffer of the Reasy Mini Kit.
Sample_growth_protocol_ch1	All patients with PCOS fulfilled the 1990 National Institutes of Health and the revised 2003 Rotterdam consensus diagnostic criteria for PCOS, and the patients which average age was 32 years (range 25-38 years) were enrolled in the study.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 to analyze the hybridization data.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Xin,,Huang
Sample_contact_email	huangxin92129@gmail.com
Sample_contact_phone	86-535-6691999-83911
Sample_contact_laboratory	Embryo Culture Lab
Sample_contact_department	Reproduction Medical Centre
Sample_contact_institute	Yuhuangding hospital of Yantai
Sample_contact_address	Yuhuangding east street 20
Sample_contact_city	Yan tai
Sample_contact_state	Shan dong
Sample_contact_zip/postal_code	264000
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992855/suppl/GSM992855_CCMII2.CEL.gz
Sample_series_id	GSE40400
Sample_data_row_count	54613

GSM992856

GPL570

Cumulus cells from oocyte at MII stage , biological rep3

Cumulus cells isolated from oocyte at stage metaphase II (MII) of PCOS patients

cell type: Cumulus cells cell cyle: metaphase II disease state: Polycystic ovary syndrome

Gene expression data from cumulus cells isolated from oocyte at MII stage

Sample_geo_accession	GSM992856
Sample_status	Public on Jun 04 2013
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Jun 04 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	All the PCOS participants included in the study were women undergoing in vitro fertilization.Ovarian stimulation and oocyte retrieval protocols were carried out as below.pituitary down-regulation was started with mid-luteal phase GnRH agonist (Diphereline 3.75mg, PharmaBiotech, Paris, France). Once adequate pituitary down-regulation was confirmed [absence of ovarian cysts>=18mm in diameter and serum estradiol (E2) levels of <50 pg/ml]. Controlled ovarian stimulation was conducted with recombinant FSH (Follitropin Alfa, Serono, London, UK). Human chorionic gonadotropin (hCG; 10,000 IU, Profasi; Serono Laboratories) was given in when two or more follicles were at least 18mm in diameter and the serum E2 levels were at least 300pg/ml per dominant follicle. Oocyte retrieval was performed 36h after hCG administration. Cumulus cells were separated from the oocyte with strippers mechanically and washed twice in cold phosphate buffer saline (Dubelcco’s medium) (Gibco, invitrogen, Paris, France) then centrifuged at 200g for 10 min. The supernatant was removed and the pellet was resuspended in RLT buffer of the Reasy Mini Kit.
Sample_growth_protocol_ch1	All patients with PCOS fulfilled the 1990 National Institutes of Health and the revised 2003 Rotterdam consensus diagnostic criteria for PCOS, and the patients which average age was 32 years (range 25-38 years) were enrolled in the study.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 to analyze the hybridization data.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Xin,,Huang
Sample_contact_email	huangxin92129@gmail.com
Sample_contact_phone	86-535-6691999-83911
Sample_contact_laboratory	Embryo Culture Lab
Sample_contact_department	Reproduction Medical Centre
Sample_contact_institute	Yuhuangding hospital of Yantai
Sample_contact_address	Yuhuangding east street 20
Sample_contact_city	Yan tai
Sample_contact_state	Shan dong
Sample_contact_zip/postal_code	264000
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992856/suppl/GSM992856_CCMII3.CEL.gz
Sample_series_id	GSE40400
Sample_data_row_count	54613

GSM992857

GPL570

Cumulus cells from oocyte at MI stage , biological rep1

Cumulus cells isolated from oocyte at stage metaphase I (MI) of PCOS patients

cell type: Cumulus cells cell cyle: metaphase I disease state: Polycystic ovary syndrome

Gene expression data from cumulus cells isolated from oocyte at MI stage

Sample_geo_accession	GSM992857
Sample_status	Public on Jun 04 2013
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Jun 04 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	All the PCOS participants included in the study were women undergoing in vitro fertilization.Ovarian stimulation and oocyte retrieval protocols were carried out as below.pituitary down-regulation was started with mid-luteal phase GnRH agonist (Diphereline 3.75mg, PharmaBiotech, Paris, France). Once adequate pituitary down-regulation was confirmed [absence of ovarian cysts>=18mm in diameter and serum estradiol (E2) levels of <50 pg/ml]. Controlled ovarian stimulation was conducted with recombinant FSH (Follitropin Alfa, Serono, London, UK). Human chorionic gonadotropin (hCG; 10,000 IU, Profasi; Serono Laboratories) was given in when two or more follicles were at least 18mm in diameter and the serum E2 levels were at least 300pg/ml per dominant follicle. Oocyte retrieval was performed 36h after hCG administration. Cumulus cells were separated from the oocyte with strippers mechanically and washed twice in cold phosphate buffer saline (Dubelcco’s medium) (Gibco, invitrogen, Paris, France) then centrifuged at 200g for 10 min. The supernatant was removed and the pellet was resuspended in RLT buffer of the Reasy Mini Kit.
Sample_growth_protocol_ch1	All patients with PCOS fulfilled the 1990 National Institutes of Health and the revised 2003 Rotterdam consensus diagnostic criteria for PCOS, and the patients which average age was 32 years (range 25-38 years) were enrolled in the study.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 to analyze the hybridization data.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Xin,,Huang
Sample_contact_email	huangxin92129@gmail.com
Sample_contact_phone	86-535-6691999-83911
Sample_contact_laboratory	Embryo Culture Lab
Sample_contact_department	Reproduction Medical Centre
Sample_contact_institute	Yuhuangding hospital of Yantai
Sample_contact_address	Yuhuangding east street 20
Sample_contact_city	Yan tai
Sample_contact_state	Shan dong
Sample_contact_zip/postal_code	264000
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992857/suppl/GSM992857_CCMI1.CEL.gz
Sample_series_id	GSE40400
Sample_data_row_count	54613

GSM992858

GPL570

Cumulus cells from oocyte at MI stage , biological rep2

Cumulus cells isolated from oocyte at stage metaphase I (MI) of PCOS patients

cell type: Cumulus cells cell cyle: metaphase I disease state: Polycystic ovary syndrome

Gene expression data from cumulus cells isolated from oocyte at MI stage

Sample_geo_accession	GSM992858
Sample_status	Public on Jun 05 2013
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Jun 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	All the PCOS participants included in the study were women undergoing in vitro fertilization.Ovarian stimulation and oocyte retrieval protocols were carried out as below.pituitary down-regulation was started with mid-luteal phase GnRH agonist (Diphereline 3.75mg, PharmaBiotech, Paris, France). Once adequate pituitary down-regulation was confirmed [absence of ovarian cysts>=18mm in diameter and serum estradiol (E2) levels of <50 pg/ml]. Controlled ovarian stimulation was conducted with recombinant FSH (Follitropin Alfa, Serono, London, UK). Human chorionic gonadotropin (hCG; 10,000 IU, Profasi; Serono Laboratories) was given in when two or more follicles were at least 18mm in diameter and the serum E2 levels were at least 300pg/ml per dominant follicle. Oocyte retrieval was performed 36h after hCG administration. Cumulus cells were separated from the oocyte with strippers mechanically and washed twice in cold phosphate buffer saline (Dubelcco’s medium) (Gibco, invitrogen, Paris, France) then centrifuged at 200g for 10 min. The supernatant was removed and the pellet was resuspended in RLT buffer of the Reasy Mini Kit.
Sample_growth_protocol_ch1	All patients with PCOS fulfilled the 1990 National Institutes of Health and the revised 2003 Rotterdam consensus diagnostic criteria for PCOS, and the patients which average age was 32 years (range 25-38 years) were enrolled in the study.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 to analyze the hybridization data.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Xin,,Huang
Sample_contact_email	huangxin92129@gmail.com
Sample_contact_phone	86-535-6691999-83911
Sample_contact_laboratory	Embryo Culture Lab
Sample_contact_department	Reproduction Medical Centre
Sample_contact_institute	Yuhuangding hospital of Yantai
Sample_contact_address	Yuhuangding east street 20
Sample_contact_city	Yan tai
Sample_contact_state	Shan dong
Sample_contact_zip/postal_code	264000
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992858/suppl/GSM992858_CCMI2.CEL.gz
Sample_series_id	GSE40400
Sample_data_row_count	54613

GSM992859

GPL570

Cumulus cells from oocyte at MI stage , biological rep3

Cumulus cells isolated from oocyte at stage metaphase I (MI) of PCOS patients

cell type: Cumulus cells cell cyle: metaphase I disease state: Polycystic ovary syndrome

Gene expression data from cumulus cells isolated from oocyte at MI stage

Sample_geo_accession	GSM992859
Sample_status	Public on Jun 05 2013
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Jun 05 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	All the PCOS participants included in the study were women undergoing in vitro fertilization.Ovarian stimulation and oocyte retrieval protocols were carried out as below.pituitary down-regulation was started with mid-luteal phase GnRH agonist (Diphereline 3.75mg, PharmaBiotech, Paris, France). Once adequate pituitary down-regulation was confirmed [absence of ovarian cysts>=18mm in diameter and serum estradiol (E2) levels of <50 pg/ml]. Controlled ovarian stimulation was conducted with recombinant FSH (Follitropin Alfa, Serono, London, UK). Human chorionic gonadotropin (hCG; 10,000 IU, Profasi; Serono Laboratories) was given in when two or more follicles were at least 18mm in diameter and the serum E2 levels were at least 300pg/ml per dominant follicle. Oocyte retrieval was performed 36h after hCG administration. Cumulus cells were separated from the oocyte with strippers mechanically and washed twice in cold phosphate buffer saline (Dubelcco’s medium) (Gibco, invitrogen, Paris, France) then centrifuged at 200g for 10 min. The supernatant was removed and the pellet was resuspended in RLT buffer of the Reasy Mini Kit.
Sample_growth_protocol_ch1	All patients with PCOS fulfilled the 1990 National Institutes of Health and the revised 2003 Rotterdam consensus diagnostic criteria for PCOS, and the patients which average age was 32 years (range 25-38 years) were enrolled in the study.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using Affymetrix GeneChip Scanner 3000 to analyze the hybridization data.
Sample_data_processing	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Sample_platform_id	GPL570
Sample_contact_name	Xin,,Huang
Sample_contact_email	huangxin92129@gmail.com
Sample_contact_phone	86-535-6691999-83911
Sample_contact_laboratory	Embryo Culture Lab
Sample_contact_department	Reproduction Medical Centre
Sample_contact_institute	Yuhuangding hospital of Yantai
Sample_contact_address	Yuhuangding east street 20
Sample_contact_city	Yan tai
Sample_contact_state	Shan dong
Sample_contact_zip/postal_code	264000
Sample_contact_country	China
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992859/suppl/GSM992859_CCMI3.CEL.gz
Sample_series_id	GSE40400
Sample_data_row_count	54613

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