Result

Search results for the GEO ID: GSE40404

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM992923

GPL570

ndf + preLA

human fibroblasts overexpressing prelamin A

passage: 10 cell type: fibroblast treatment: overexpressing prelamin A cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992923
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992923/suppl/GSM992923_lucioC_J140_A_HG_U133_Plus_2_01_1_LA3_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

GSM992924

GPL570

ndf + preLA + ZMPSTE24

human fibroblasts overexpressing prelamin A and ZMPSTE24

passage: 10 cell type: fibroblast treatment: overexpressing prelamin A and ZMPSTE24 cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992924
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992924/suppl/GSM992924_lucioC_J141_A_HG_U133_Plus_2_01_1_LA_FACE1_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

GSM992925

GPL570

ndf + FTI

human fibroblasts overexpressing prelamin A treated with FTI

passage: 10 cell type: fibroblast treatment: overexpressing prelamin A treated with FTI cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992925
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992925/suppl/GSM992925_lucioC_J142_A_HG_U133_Plus_2_01_1_LA_FT1_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

GSM992926

GPL570

ndf control1

human fibroblasts

passage: 10 cell type: fibroblast cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992926
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992926/suppl/GSM992926_lucioC_J143_A_HG_U133_Plus_2_01_1_pkey_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

GSM992927

GPL570

ndf control 2

human fibroblasts

passage: 10 cell type: fibroblast cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992927
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992927/suppl/GSM992927_lucioC_J468_A_HG_U133_Plus_2_01_1_PKEYP8_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

GSM992928

GPL570

ndf + progerin

human fibroblasts overexpressing progerin

passage: 10 cell type: fibroblast treatment: overexpressing progerin cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992928
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992928/suppl/GSM992928_lucioC_J471_A_HG_U133_Plus_2_01_1_HGPS_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

GSM992929

GPL570

ndf + progerin + FTI

human fibroblasts overexpressing progerin treated with FTI

passage: 10 cell type: fibroblast treatment: overexpressing progerin treated with FTI cell line: GM00038

Gene Expression data

Sample_geo_accession	GSM992929
Sample_status	Public on Aug 31 2012
Sample_submission_date	Aug 27 2012
Sample_last_update_date	Oct 17 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM00038
Sample_treatment_protocol_ch1	When indicated, cells were grown in DMEM supplemented with either 10 nm farnesyltransferase inhibitor L-744832 (Biomol, Plymouth Meeting, PA, USA) or DMSO and 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Media was changed every 4 days.
Sample_growth_protocol_ch1	Primary dermal fibroblast cell line (GM00038) from healthy individual was obtained from the Coriell Cell Repository. Cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum, 2 mm l-glutamine, 100 U mL−1 penicillin and 100 µg mL−1 streptomycin at 37 °C in 5% CO2, and 3% O2. Cells seeded at 1.4 × 10exp5 per 100-mm-diameter dish were passaged when cultures reached 85% confluency.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from each fibroblast line at passage 10 using RNeasy kit from QIAGEN according to the manufacture’s protocol and quantitated by assessing absorbance at 260 and 280 nm using a NanoDropTM 1000 spectrophotometer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	standard Affymetrix protocol
Sample_hyb_protocol	Three micrograms of total RNA was then submitted to the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles for processing, chip hybridization, and scanning. Gene expression was analyzed on an Affimetrix gene chip Human Genome U133 Plus 2.0 Array,
Sample_scan_protocol	A Fluidics Station 400 (Affymetrix) was used to wash and stain the chips and fluorescence was detected using a G2500 GeneArray Scanner (Hewlett-Packard).
Sample_data_processing	Raw data were analyzed initially using Microarray Suite version 5.0 (MAS 5.0, Affymetrix)
Sample_platform_id	GPL570
Sample_contact_name	Lucio,,Comai
Sample_contact_email	comai@usc.edu
Sample_contact_institute	University of Southern California
Sample_contact_address	2250 Alcazar Street
Sample_contact_city	Los Angeles
Sample_contact_zip/postal_code	90033
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM992nnn/GSM992929/suppl/GSM992929_lucioC_J472_A_HG_U133_Plus_2_01_1_HGPSFTI_.CEL.gz
Sample_series_id	GSE40404
Sample_data_row_count	54675

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