Search results for the GEO ID: GSE40413  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM993399 | GPL570 |
|
24h Ctrl
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: Ctrl
time point: 24 hour cytokine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993399
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993399/suppl/GSM993399_1_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993400 | GPL570 |
|
24h TNF (10ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)
time point: 24 hour cytokine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993400
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993400/suppl/GSM993400_2_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993401 | GPL570 |
|
24h IL-17 (20ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: IL-17 (20ng/mL)
time point: 24 hour cytokine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993401
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993401/suppl/GSM993401_3_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993402 | GPL570 |
|
24h IL-17 (200ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: IL-17 (200ng/mL)
time point: 24 hour cytokine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993402
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993402/suppl/GSM993402_4_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993403 | GPL570 |
|
24h TNF (10ng/mL)+IL-17 (20ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)+IL-17 (20ng/mL)
time point: 24 hour cytokine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993403
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993403/suppl/GSM993403_5_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993404 | GPL570 |
|
24h TNF (10ng/mL)+IL17 (200ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)+IL-17 (200ng/mL)
time point: 24 hour cytokine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993404
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993404/suppl/GSM993404_6_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993405 | GPL570 |
|
48h Ctrl
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: Ctrl
time point: 48 hour cyotkine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993405
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993405/suppl/GSM993405_7_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993406 | GPL570 |
|
48h TNF (10ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)
time point: 48 hour cyotkine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993406
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993406/suppl/GSM993406_8_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993407 | GPL570 |
|
48h IL-17 (20ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: IL-17 (20ng/mL)
time point: 48 hour cyotkine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993407
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993407/suppl/GSM993407_9_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993408 | GPL570 |
|
48h IL-17 (200ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: IL-17 (200ng/mL)
time point: 48 hour cyotkine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993408
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993408/suppl/GSM993408_10_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993409 | GPL570 |
|
48h TNF (10ng/mL)+IL-17 (20ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)+IL-17 (20ng/mL)
time point: 48 hour cyotkine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993409
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993409/suppl/GSM993409_11_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
GSM993410 | GPL570 |
|
48h TNF (10ng/mL)+IL-17 (200ng/mL)
|
normal human epidermal melanocyte
|
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)+IL-17 (200ng/mL)
time point: 48 hour cyotkine treatment
amplification: single round
|
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Sample_geo_accession | GSM993410
| | Sample_status | Public on Jun 15 2013
| | Sample_submission_date | Aug 27 2012
| | Sample_last_update_date | Jun 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
| | Sample_growth_protocol_ch1 | Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | QIAGEN Rneasy Kit
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix single amplification protocol
| | Sample_hyb_protocol | Affymetrix protocol
| | Sample_scan_protocol | Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
| | Sample_data_processing | GCRMA
| | Sample_data_processing | Normalized Expression values in log-2 scale
| | Sample_platform_id | GPL570
| | Sample_contact_name | Claire,Q.F.,Wang
| | Sample_contact_email | qwang@rockefeller.edu
| | Sample_contact_phone | +1-212-327-7153
| | Sample_contact_fax | +1-212-327-8232
| | Sample_contact_laboratory | Laboratory for Investigative Dermatology
| | Sample_contact_institute | Rockefeller University
| | Sample_contact_address | 1230 York Ave
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993410/suppl/GSM993410_12_HGU133_plus_2.0_Claire_091411.CEL.gz
| | Sample_series_id | GSE40413
| | Sample_data_row_count | 54675
| | |
|
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