Search results for the GEO ID: GSE40422  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM993781 | GPL570 |
|
SkMel30 cells with GFP/control 1
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SkMel30 cells transduced with GFP control
|
cell line: SkMel30
transduction: GFP control
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SkMel30 cells transduced with GFP control
|
| Sample_geo_accession | GSM993781
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Aug 28 2012
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Full-length cDNA encoding HOXA1 (NM_005522.4) was obtained from the human ORFeome collection and transferred to viral vectors via Gateway recombination. The SkMel30 melanoma cell lines was transduced with either control or HOXA1 vector.
| | Sample_growth_protocol_ch1 | All cell lines were propagated at 37°C and 5% CO2 in humidified atmosphere in RPMI 1640 Medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using TRIzol Reagent (Invitrogen) and then cDNA was made from total RNA with SuperScript II (Invitrogen) using random primers. cDNA was treated with RNase H (Invitrogen) to remove RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on U133_Plus2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and quantile normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chad,,Creighton
| | Sample_contact_email | creighto@bcm.tmc.edu
| | Sample_contact_department | Biostatistics, Ducan Cancer Center
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993781/suppl/GSM993781_01_GFP_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE40422
| | Sample_data_row_count | 54675
| | |
|
GSM993782 | GPL570 |
|
SkMel30 cells with GFP/control 2
|
SkMel30 cells transduced with GFP control
|
cell line: SkMel30
transduction: GFP control
|
SkMel30 cells transduced with GFP control
|
| Sample_geo_accession | GSM993782
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Aug 28 2012
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Full-length cDNA encoding HOXA1 (NM_005522.4) was obtained from the human ORFeome collection and transferred to viral vectors via Gateway recombination. The SkMel30 melanoma cell lines was transduced with either control or HOXA1 vector.
| | Sample_growth_protocol_ch1 | All cell lines were propagated at 37°C and 5% CO2 in humidified atmosphere in RPMI 1640 Medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using TRIzol Reagent (Invitrogen) and then cDNA was made from total RNA with SuperScript II (Invitrogen) using random primers. cDNA was treated with RNase H (Invitrogen) to remove RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on U133_Plus2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and quantile normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chad,,Creighton
| | Sample_contact_email | creighto@bcm.tmc.edu
| | Sample_contact_department | Biostatistics, Ducan Cancer Center
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993782/suppl/GSM993782_02_GFP_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE40422
| | Sample_data_row_count | 54675
| | |
|
GSM993783 | GPL570 |
|
SkMel30 cells with GFP/control 3
|
SkMel30 cells transduced with GFP control
|
cell line: SkMel30
transduction: GFP control
|
SkMel30 cells transduced with GFP control
|
| Sample_geo_accession | GSM993783
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Aug 28 2012
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Full-length cDNA encoding HOXA1 (NM_005522.4) was obtained from the human ORFeome collection and transferred to viral vectors via Gateway recombination. The SkMel30 melanoma cell lines was transduced with either control or HOXA1 vector.
| | Sample_growth_protocol_ch1 | All cell lines were propagated at 37°C and 5% CO2 in humidified atmosphere in RPMI 1640 Medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using TRIzol Reagent (Invitrogen) and then cDNA was made from total RNA with SuperScript II (Invitrogen) using random primers. cDNA was treated with RNase H (Invitrogen) to remove RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on U133_Plus2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and quantile normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chad,,Creighton
| | Sample_contact_email | creighto@bcm.tmc.edu
| | Sample_contact_department | Biostatistics, Ducan Cancer Center
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993783/suppl/GSM993783_03_GFP_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE40422
| | Sample_data_row_count | 54675
| | |
|
GSM993784 | GPL570 |
|
SkMel30 cells with HOXA1 4
|
SkMel30 cells transduced with HOXA1
|
cell line: SkMel30
transduction: HOXA1
|
SkMel30 cells transduced with HOXA1
|
| Sample_geo_accession | GSM993784
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Aug 28 2012
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Full-length cDNA encoding HOXA1 (NM_005522.4) was obtained from the human ORFeome collection and transferred to viral vectors via Gateway recombination. The SkMel30 melanoma cell lines was transduced with either control or HOXA1 vector.
| | Sample_growth_protocol_ch1 | All cell lines were propagated at 37°C and 5% CO2 in humidified atmosphere in RPMI 1640 Medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using TRIzol Reagent (Invitrogen) and then cDNA was made from total RNA with SuperScript II (Invitrogen) using random primers. cDNA was treated with RNase H (Invitrogen) to remove RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on U133_Plus2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and quantile normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chad,,Creighton
| | Sample_contact_email | creighto@bcm.tmc.edu
| | Sample_contact_department | Biostatistics, Ducan Cancer Center
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993784/suppl/GSM993784_04_HOX41_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE40422
| | Sample_data_row_count | 54675
| | |
|
GSM993785 | GPL570 |
|
SkMel30 cells with HOXA1 5
|
SkMel30 cells transduced with HOXA1
|
cell line: SkMel30
transduction: HOXA1
|
SkMel30 cells transduced with HOXA1
|
| Sample_geo_accession | GSM993785
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Aug 28 2012
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Full-length cDNA encoding HOXA1 (NM_005522.4) was obtained from the human ORFeome collection and transferred to viral vectors via Gateway recombination. The SkMel30 melanoma cell lines was transduced with either control or HOXA1 vector.
| | Sample_growth_protocol_ch1 | All cell lines were propagated at 37°C and 5% CO2 in humidified atmosphere in RPMI 1640 Medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using TRIzol Reagent (Invitrogen) and then cDNA was made from total RNA with SuperScript II (Invitrogen) using random primers. cDNA was treated with RNase H (Invitrogen) to remove RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on U133_Plus2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and quantile normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chad,,Creighton
| | Sample_contact_email | creighto@bcm.tmc.edu
| | Sample_contact_department | Biostatistics, Ducan Cancer Center
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993785/suppl/GSM993785_05_HOX41_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE40422
| | Sample_data_row_count | 54675
| | |
|
GSM993786 | GPL570 |
|
SkMel30 cells with HOXA1 6
|
SkMel30 cells transduced with HOXA1
|
cell line: SkMel30
transduction: HOXA1
|
SkMel30 cells transduced with HOXA1
|
| Sample_geo_accession | GSM993786
| | Sample_status | Public on Jan 01 2013
| | Sample_submission_date | Aug 28 2012
| | Sample_last_update_date | Jan 01 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Full-length cDNA encoding HOXA1 (NM_005522.4) was obtained from the human ORFeome collection and transferred to viral vectors via Gateway recombination. The SkMel30 melanoma cell lines was transduced with either control or HOXA1 vector.
| | Sample_growth_protocol_ch1 | All cell lines were propagated at 37°C and 5% CO2 in humidified atmosphere in RPMI 1640 Medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from cells using TRIzol Reagent (Invitrogen) and then cDNA was made from total RNA with SuperScript II (Invitrogen) using random primers. cDNA was treated with RNase H (Invitrogen) to remove RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized on U133_Plus2 GeneChip following the recommended procedures. GeneChips were washed and stained according to Affymetrix protocol EukGE-WS2v5 (for FS400) or FS450_0001 (for FS450).
| | Sample_scan_protocol | GeneChips were scanned using an Affymetrix GeneChip Scanner (Agilent).
| | Sample_data_processing | The data were analyzed with dChip version2006 using Affymetrix default analysis settings and quantile normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chad,,Creighton
| | Sample_contact_email | creighto@bcm.tmc.edu
| | Sample_contact_department | Biostatistics, Ducan Cancer Center
| | Sample_contact_institute | Baylor College of Medicine
| | Sample_contact_address | One Baylor Plaza, Mail Stop: BCM305
| | Sample_contact_city | Houston
| | Sample_contact_state | TX
| | Sample_contact_zip/postal_code | 77030
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993786/suppl/GSM993786_06_HOX41_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE40422
| | Sample_data_row_count | 54675
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