Search results for the GEO ID: GSE40424 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM993788 | GPL570 |
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A549 NC
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A549 cells transfected by scramble siRNA
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source tissue: lung
cell line: A549
treatment: hMOF siRNA
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Sample_geo_accession | GSM993788
| Sample_status | Public on Aug 29 2012
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Aug 29 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | H1299 and A549 cells were transfected with siRNA using Lipofectamine 2000 reagent.
| Sample_growth_protocol_ch1 | H1299 and A549 cell lines were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS) and cultured in a humidified atmosphere in an incubator at 37°C with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted by Trizol reagent.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Arrays scanned using a confocal scanner LuxScanTM 10K/A (CapitalBio),and the data of obtained images were extracted with the LuxScan3.0 software (CapitalBio).
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Lei,,Zhao
| Sample_contact_email | alei831@yahoo.com.cn
| Sample_contact_institute | Tsinghua University
| Sample_contact_address | School of Medicine,Tsinghua University
| Sample_contact_city | Beijing
| Sample_contact_zip/postal_code | 100084
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993788/suppl/GSM993788_100720-10398A_A549NC_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE40424
| Sample_data_row_count | 54675
| |
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GSM993789 | GPL570 |
|
A549 siM
|
A549 cells transfected by hMOF siRNA
|
source tissue: lung
cell line: A549
treatment: hMOF siRNA
|
|
Sample_geo_accession | GSM993789
| Sample_status | Public on Aug 29 2012
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Aug 29 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | H1299 and A549 cells were transfected with siRNA using Lipofectamine 2000 reagent.
| Sample_growth_protocol_ch1 | H1299 and A549 cell lines were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS) and cultured in a humidified atmosphere in an incubator at 37°C with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted by Trizol reagent.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Arrays scanned using a confocal scanner LuxScanTM 10K/A (CapitalBio),and the data of obtained images were extracted with the LuxScan3.0 software (CapitalBio).
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Lei,,Zhao
| Sample_contact_email | alei831@yahoo.com.cn
| Sample_contact_institute | Tsinghua University
| Sample_contact_address | School of Medicine,Tsinghua University
| Sample_contact_city | Beijing
| Sample_contact_zip/postal_code | 100084
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993789/suppl/GSM993789_100720-10398A_A549siM_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE40424
| Sample_data_row_count | 54675
| |
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GSM993790 | GPL570 |
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H1299 NC
|
H1299 cells transfected by scramble siRNA
|
source tissue: lung
cell line: H1299
treatment: control siRNA
|
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Sample_geo_accession | GSM993790
| Sample_status | Public on Aug 29 2012
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Aug 29 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | H1299 and A549 cells were transfected with siRNA using Lipofectamine 2000 reagent.
| Sample_growth_protocol_ch1 | H1299 and A549 cell lines were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS) and cultured in a humidified atmosphere in an incubator at 37°C with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted by Trizol reagent.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Arrays scanned using a confocal scanner LuxScanTM 10K/A (CapitalBio),and the data of obtained images were extracted with the LuxScan3.0 software (CapitalBio).
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Lei,,Zhao
| Sample_contact_email | alei831@yahoo.com.cn
| Sample_contact_institute | Tsinghua University
| Sample_contact_address | School of Medicine,Tsinghua University
| Sample_contact_city | Beijing
| Sample_contact_zip/postal_code | 100084
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993790/suppl/GSM993790_100720-10398A_H1299NC_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE40424
| Sample_data_row_count | 54675
| |
|
GSM993791 | GPL570 |
|
H1299 siM
|
H1299 cells transfected by hMOF siRNA
|
source tissue: lung
cell line: H1299
treatment: control siRNA
|
|
Sample_geo_accession | GSM993791
| Sample_status | Public on Aug 29 2012
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Aug 29 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | H1299 and A549 cells were transfected with siRNA using Lipofectamine 2000 reagent.
| Sample_growth_protocol_ch1 | H1299 and A549 cell lines were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS) and cultured in a humidified atmosphere in an incubator at 37°C with 5% CO2.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA were extracted by Trizol reagent.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | Arrays scanned using a confocal scanner LuxScanTM 10K/A (CapitalBio),and the data of obtained images were extracted with the LuxScan3.0 software (CapitalBio).
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Lei,,Zhao
| Sample_contact_email | alei831@yahoo.com.cn
| Sample_contact_institute | Tsinghua University
| Sample_contact_address | School of Medicine,Tsinghua University
| Sample_contact_city | Beijing
| Sample_contact_zip/postal_code | 100084
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM993nnn/GSM993791/suppl/GSM993791_100720-10398A_H1299siM_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE40424
| Sample_data_row_count | 54675
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