Search results for the GEO ID: GSE40438 |
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(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM994202 | GPL570 |
|
Lumbar spinal motor neurons 1
|
Lumbar spinal motor neurons, control case 0507
|
post-mortem control case: 0507
cell type: lumbar spinal motor neurons
disease state: normal control
|
Lumbar 0507
Gene expression data from lumbar spinal motor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994202
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994202/suppl/GSM994202_Lumbar_0507.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994203 | GPL570 |
|
Lumbar spinal motor neurons 2
|
Lumbar spinal motor neurons, control case 1207
|
post-mortem control case: 1207
cell type: lumbar spinal motor neurons
disease state: normal control
|
Lumbar 1207
Gene expression data from lumbar spinal motor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994203
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994203/suppl/GSM994203_Lumbar_1207.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994204 | GPL570 |
|
Lumbar spinal motor neurons 3
|
Lumbar spinal motor neurons, control case 8507
|
post-mortem control case: 8507
cell type: lumbar spinal motor neurons
disease state: normal control
|
Lumbar 8507
Gene expression data from lumbar spinal motor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994204
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994204/suppl/GSM994204_Lumbar_8507.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994205 | GPL570 |
|
Lumbar spinal motor neurons 4
|
Lumbar spinal motor neurons, control case 9807
|
post-mortem control case: 9807
cell type: lumbar spinal motor neurons
disease state: normal control
|
Lumbar 9807
Gene expression data from lumbar spinal motor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994205
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994205/suppl/GSM994205_Lumbar_9807.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994206 | GPL570 |
|
Oculomotor neurons 1
|
Oculomotor neurons, control case 0507
|
post-mortem control case: 0507
cell type: oculomotor neurons
disease state: normal control
|
Oculomotor 0507
Gene expression data from oculomotor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994206
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994206/suppl/GSM994206_Oculomotor_0507.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994207 | GPL570 |
|
Oculomotor neurons 2
|
Oculomotor neurons, control case 1207
|
post-mortem control case: 1207
cell type: oculomotor neurons
disease state: normal control
|
Oculomotor 1207
Gene expression data from oculomotor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994207
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994207/suppl/GSM994207_Oculomotor_1207.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994208 | GPL570 |
|
Oculomotor neurons 3
|
Oculomotor neurons, control case 8507
|
post-mortem control case: 8507
cell type: oculomotor neurons
disease state: normal control
|
Oculomotor 8507
Gene expression data from oculomotor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994208
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994208/suppl/GSM994208_Oculomotor_8507.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
|
GSM994209 | GPL570 |
|
Oculomotor neurons 4
|
Oculomotor neurons, control case 9807
|
post-mortem control case: 9807
cell type: oculomotor neurons
disease state: normal control
|
Oculomotor 9807
Gene expression data from oculomotor neurons from human post-mortem control case.
|
Sample_geo_accession | GSM994209
| Sample_status | Public on Mar 01 2013
| Sample_submission_date | Aug 28 2012
| Sample_last_update_date | Mar 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | 10 um lumbar spinal cord or midbrain frozen sections were fixed, washed and stained with toluidine blue. They were then washed and dehydrated through graded ethanol concentrations (70%, 90%, 100%) and xylene.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | From each case, and each tissue type, 500-1000 motor neurons (MN) were isolated using a PixCell II laser-capture microdissection system (Arcturus Bioscience).
| Sample_extract_protocol_ch1 | The PicoPure RNA Extraction Kit was used for RNA extraction according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylation of antisense RNA was carried out according to the Affymetrix protocol.
| Sample_hyb_protocol | 15µg of cRNA molecules were heat fragmented and applied to the Human Genome U133 Plus 2.0 Array (Affymetrix) in a hybridisation solution according to the Affymetrix protocol. Hybridisation took place overnight in a rotating hybridisation platform at 45°C for 16 hours. Post-hybridisation stringency washing was done using the Affymetrix Fluidics Station again following the protocol outlined in the Affymetrix instructions.
| Sample_scan_protocol | The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image was processed using the GCOS software package to prepare the .CEL files.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| Sample_platform_id | GPL570
| Sample_contact_name | Paul,Roy,Heath
| Sample_contact_email | p.heath@sheffield.ac.uk
| Sample_contact_phone | +44 114 2222254
| Sample_contact_laboratory | Heath
| Sample_contact_department | Academic Neurology Unit
| Sample_contact_institute | University of Sheffield
| Sample_contact_address | SITraN, 385a Glossop Road
| Sample_contact_city | Sheffield
| Sample_contact_state | S. Yorks
| Sample_contact_zip/postal_code | S10 2HQ
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM994nnn/GSM994209/suppl/GSM994209_Oculomotor_9807.CEL.gz
| Sample_series_id | GSE40438
| Sample_data_row_count | 54675
| |
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