Result

Search results for the GEO ID: GSE40564

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM996643

GPL570

H69_Ctr_1

SCLC cells

cell line: H69 treatment: none

Sample_geo_accession	GSM996643
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996643/suppl/GSM996643_caquinof_20090624_H69_Ctr_1.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996644

GPL570

H69_Ctr_3

SCLC cells

cell line: H69 treatment: none

Sample_geo_accession	GSM996644
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996644/suppl/GSM996644_caquinof_20090624_H69_Ctr_3.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996645

GPL570

H69_Ctr_5

SCLC cells

cell line: H69 treatment: none

Sample_geo_accession	GSM996645
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996645/suppl/GSM996645_caquinof_20090624_H69_Ctr_5.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996646

GPL570

H69_PIK75_1

SCLC cells

cell line: H69 treatment: PIK75

Sample_geo_accession	GSM996646
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996646/suppl/GSM996646_caquinof_20090624_H69_PIK75_1.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996647

GPL570

H69_PIK75_3

SCLC cells

cell line: H69 treatment: PIK75

Sample_geo_accession	GSM996647
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996647/suppl/GSM996647_caquinof_20090624_H69_PIK75_3.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996648

GPL570

H69_PIK75_5

SCLC cells

cell line: H69 treatment: PIK75

Sample_geo_accession	GSM996648
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996648/suppl/GSM996648_caquinof_20090624_H69_PIK75_5.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996649

GPL570

H69_TGX221_2

SCLC cells

cell line: H69 treatment: TGX221

Sample_geo_accession	GSM996649
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996649/suppl/GSM996649_caquinof_20090624_H69_TGX221_2.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996650

GPL570

H69_TGX221_3

SCLC cells

cell line: H69 treatment: TGX221

Sample_geo_accession	GSM996650
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996650/suppl/GSM996650_caquinof_20090624_H69_TGX221_3.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

GSM996651

GPL570

H69_TGX221_5

SCLC cells

cell line: H69 treatment: TGX221

Sample_geo_accession	GSM996651
Sample_status	Public on Dec 01 2012
Sample_submission_date	Sep 04 2012
Sample_last_update_date	Dec 01 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	H69 cells were treated with DMSO (control), PIK75 (100 nM) or TGX221 (1 mM) for 24 hrs at 37 °C in complete RPMI medium.
Sample_growth_protocol_ch1	The human SCLC cell line H69 was cultured in RPMI medium containing 10% heat-inactivated FCS, 1% L-Glutamine and 1% penicillin/streptomycin at 37°C/5% CO2 (humidified atmosphere).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total cellular RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.
Sample_hyb_protocol	10 μg of cRNA/sample were hybridized for 16 h at 45°C. Washing and staining with streptavidin-conjugated phycoerythrin were done using a GeneChip Fluidics Station 400 (Affymetrix).
Sample_scan_protocol	Scanning was done with the GeneChip® Scanner 3000 7G (Affymetrix) using the Affymetrix Command Console as instrument control software.
Sample_data_processing	Quality control and microarray data analysis was done using R/Bioconductor [Gentleman RC et al., 2004; R Development Core Team, 2011; http://www.bioconductor.org/]. Expression values were computed RMA (robust multi array average) [Irizarry et al., 2003a, b] as processing algorithm.
Sample_platform_id	GPL570
Sample_contact_name	Hubert,,Rehrauer
Sample_contact_email	Hubert.Rehrauer@fgcz.ethz.ch
Sample_contact_institute	FGCZ
Sample_contact_address	Winterthurerstr. 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996651/suppl/GSM996651_caquinof_20090624_H69_TGX221_5.CEL.gz
Sample_series_id	GSE40564
Sample_data_row_count	54675

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