Search results for the GEO ID: GSE40567 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM996763 | GPL570 |
|
Control-rep1
|
U-2 OS cells expressing empty vector
|
cell line: U-2 OS
genotype/variation: expressing empty vector
|
|
Sample_geo_accession | GSM996763
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996763/suppl/GSM996763_JQ2009061201.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996764 | GPL570 |
|
T1-rep1
|
U-2 OS cells expressing full-length SV40 LT
|
cell line: U-2 OS
genotype/variation: expressing full-length SV40 LT
|
|
Sample_geo_accession | GSM996764
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996764/suppl/GSM996764_JQ2009061202.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996765 | GPL570 |
|
T6-rep1
|
U-2 OS cells expressing SV40 LT fragment T6
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T6
|
|
Sample_geo_accession | GSM996765
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996765/suppl/GSM996765_JQ2009061203.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996766 | GPL570 |
|
T8-rep1
|
U-2 OS cells expressing SV40 LT fragment T8
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T8
|
|
Sample_geo_accession | GSM996766
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996766/suppl/GSM996766_JQ2009061204.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996767 | GPL570 |
|
T16-rep1
|
U-2 OS cells expressing SV40 LT fragment T16
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T16
|
|
Sample_geo_accession | GSM996767
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996767/suppl/GSM996767_JQ2009061205.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996768 | GPL570 |
|
Control-rep2
|
U-2 OS cells expressing empty vector
|
cell line: U-2 OS
genotype/variation: expressing empty vector
|
|
Sample_geo_accession | GSM996768
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996768/suppl/GSM996768_JQ2009061219.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996769 | GPL570 |
|
T1-rep2
|
U-2 OS cells expressing full-length SV40 LT
|
cell line: U-2 OS
genotype/variation: expressing full-length SV40 LT
|
|
Sample_geo_accession | GSM996769
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996769/suppl/GSM996769_JQ2009061220.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996770 | GPL570 |
|
T6-rep2
|
U-2 OS cells expressing SV40 LT fragment T6
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T6
|
|
Sample_geo_accession | GSM996770
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996770/suppl/GSM996770_JQ2009061221.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996771 | GPL570 |
|
T8-rep2
|
U-2 OS cells expressing SV40 LT fragment T8
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T8
|
|
Sample_geo_accession | GSM996771
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996771/suppl/GSM996771_JQ2009061222.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996772 | GPL570 |
|
T16-rep2
|
U-2 OS cells expressing SV40 LT fragment T16
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T16
|
|
Sample_geo_accession | GSM996772
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996772/suppl/GSM996772_JQ2009061223.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996773 | GPL570 |
|
Control-rep3
|
U-2 OS cells expressing empty vector
|
cell line: U-2 OS
genotype/variation: expressing empty vector
|
|
Sample_geo_accession | GSM996773
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996773/suppl/GSM996773_JQ2009061237.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996774 | GPL570 |
|
T1-rep3
|
U-2 OS cells expressing full-length SV40 LT
|
cell line: U-2 OS
genotype/variation: expressing full-length SV40 LT
|
|
Sample_geo_accession | GSM996774
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996774/suppl/GSM996774_JQ2009061238.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
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GSM996775 | GPL570 |
|
T6-rep3
|
U-2 OS cells expressing SV40 LT fragment T6
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T6
|
|
Sample_geo_accession | GSM996775
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996775/suppl/GSM996775_JQ2009061239.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996776 | GPL570 |
|
T8-rep3
|
U-2 OS cells expressing SV40 LT fragment T8
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T8
|
|
Sample_geo_accession | GSM996776
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996776/suppl/GSM996776_JQ2009061240.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
|
GSM996777 | GPL570 |
|
T16-rep3
|
U-2 OS cells expressing SV40 LT fragment T16
|
cell line: U-2 OS
genotype/variation: expressing SV40 LT fragment T16
|
|
Sample_geo_accession | GSM996777
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Sep 04 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_growth_protocol_ch1 | U-2 OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cellgro) supplemented with 10% Fetal Clone-I serum (HyClone), penicillin and streptomycin.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from LT-expressing U-2 OS cell lines was extracted using TRIzol (Invitrogen) and purified in RNeasy columns (Qiagen). RNA integrity was determined using a Bioanalyzer (Agilent).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA was converted into cDNA, then amplified and labeled with biotinylated nucleotides by in vitro transcription to produce biotinylated cRNA, according to the standard Affymetrix protocol.
| Sample_hyb_protocol | Samples were hybridized to Human Gene U133 Plus 2.0 arrays according to the manufacturer's instructions.
| Sample_scan_protocol | Scanning of microarrays was performed by following Affymetrix protocols at the Dana-Farber Cancer Institute microarray core facility.
| Sample_data_processing | Data were normalized by RMA using R/Bioconductor package affy.
| Sample_platform_id | GPL570
| Sample_contact_name | Megha,,Padi
| Sample_contact_email | mpadi@jimmy.harvard.edu
| Sample_contact_department | Biostatistics and Computational Biology
| Sample_contact_institute | Dana-Farber Cancer Institute
| Sample_contact_address | 450 Brookline Avenue
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02215
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM996nnn/GSM996777/suppl/GSM996777_JQ2009061241.CEL.gz
| Sample_series_id | GSE40567
| Sample_data_row_count | 54675
| |
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