Search results for the GEO ID: GSE40839  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1003058 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep1
|
Primary pulmonary fibroblasts from control individual 1
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003058
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003058/suppl/GSM1003058_C1.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003059 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep2
|
Primary pulmonary fibroblasts from control individual 2
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003059
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003059/suppl/GSM1003059_C2.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003060 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep3
|
Primary pulmonary fibroblasts from control individual 3
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003060
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003060/suppl/GSM1003060_C3.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003061 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep4
|
Primary pulmonary fibroblasts from control individual 4
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003061
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003061/suppl/GSM1003061_C4.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003062 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep5
|
Primary pulmonary fibroblasts from control individual 5
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003062
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003062/suppl/GSM1003062_C5.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003063 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep6
|
Primary pulmonary fibroblasts from control individual 6
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003063
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003063/suppl/GSM1003063_C6.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003064 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep7
|
Primary pulmonary fibroblasts from control individual 7
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003064
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003064/suppl/GSM1003064_C7.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003065 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep8
|
Primary pulmonary fibroblasts from control individual 8
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003065
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003065/suppl/GSM1003065_C8.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003066 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep9
|
Primary pulmonary fibroblasts from control individual 9
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003066
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003066/suppl/GSM1003066_C9.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003067 | GPL96 |
|
Pulmonary fibroblasts_control individual_ biological rep10
|
Primary pulmonary fibroblasts from control individual 10
|
tissue: Pulmonary fibroblasts
disease status: Control
|
Gene expression data from pulmonary fibroblasts in individuals without lung disease.
|
| Sample_geo_accession | GSM1003067
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003067/suppl/GSM1003067_C10.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003068 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep1
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 1
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003068
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003068/suppl/GSM1003068_S1.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003069 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep2
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 2
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003069
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003069/suppl/GSM1003069_S2.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003070 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep3
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 3
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003070
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003070/suppl/GSM1003070_S3.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003071 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep4
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 4
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003071
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003071/suppl/GSM1003071_S4.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003072 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep5
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 5
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003072
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003072/suppl/GSM1003072_S5.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003073 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep6
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 6
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003073
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003073/suppl/GSM1003073_S6.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003074 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep7
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 7
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003074
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003074/suppl/GSM1003074_S7.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003075 | GPL96 |
|
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep8
|
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 8
|
tissue: Pulmonary fibroblasts
disease status: Scleroderma associated interstitial lung disease
|
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
| Sample_geo_accession | GSM1003075
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003075/suppl/GSM1003075_S8.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003076 | GPL96 |
|
Pulmonary fibroblasts_Usual Interstitial Pneumonia_ biological rep1
|
Primary pulmonary fibroblasts from Usual Interstitial Pneumonia patient 1
|
tissue: Pulmonary fibroblasts
disease status: Usual Interstitial Pneumonia
|
Gene expression data from pulmonary fibroblasts in individuals with Usual Interstitial Pneumonia.
|
| Sample_geo_accession | GSM1003076
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003076/suppl/GSM1003076_U1.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003077 | GPL96 |
|
Pulmonary fibroblasts_Usual Interstitial Pneumonia_ biological rep2
|
Primary pulmonary fibroblasts from Usual Interstitial Pneumonia patient 2
|
tissue: Pulmonary fibroblasts
disease status: Usual Interstitial Pneumonia
|
Gene expression data from pulmonary fibroblasts in individuals with Usual Interstitial Pneumonia.
|
| Sample_geo_accession | GSM1003077
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003077/suppl/GSM1003077_U2.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
GSM1003078 | GPL96 |
|
Pulmonary fibroblasts_Usual Interstitial Pneumonia_ biological rep3
|
Primary pulmonary fibroblasts from Usual Interstitial Pneumonia patient 3
|
tissue: Pulmonary fibroblasts
disease status: Usual Interstitial Pneumonia
|
Gene expression data from pulmonary fibroblasts in individuals with Usual Interstitial Pneumonia.
|
| Sample_geo_accession | GSM1003078
| | Sample_status | Public on Aug 05 2013
| | Sample_submission_date | Sep 12 2012
| | Sample_last_update_date | Aug 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
| | Sample_growth_protocol_ch1 | Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 30007G scanner.
| | Sample_data_processing | The data were analyzed with dCHIP using the invariant set normalisation method.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Carmel,,Stock
| | Sample_contact_email | c.stock@imperial.ac.uk
| | Sample_contact_department | Interstitial Lung Disease
| | Sample_contact_institute | National Heart and Lung Institute
| | Sample_contact_address | 1b Manresa Road
| | Sample_contact_city | London
| | Sample_contact_zip/postal_code | SW3 6LR
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1003nnn/GSM1003078/suppl/GSM1003078_U3.cel.gz
| | Sample_series_id | GSE40839
| | Sample_data_row_count | 22215
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
