Search results for the GEO ID: GSE40968 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1006127 | GPL570 |
|
MCF-7 Tet-On control 1
|
MCF-7 Tet-On cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: control
|
Gene expression data
|
Sample_geo_accession | GSM1006127
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006127/suppl/GSM1006127_MCF_TET_C1.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006128 | GPL570 |
|
MCF-7 Tet-On control 2
|
MCF-7 Tet-On cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: control
|
Gene expression data
|
Sample_geo_accession | GSM1006128
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006128/suppl/GSM1006128_MCF_TET_C2.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006129 | GPL570 |
|
MCF-7 Tet-On control 3
|
MCF-7 Tet-On cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: control
|
Gene expression data
|
Sample_geo_accession | GSM1006129
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006129/suppl/GSM1006129_MCF_TET_C3.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006130 | GPL570 |
|
MCF-7 Tet-On ACSL4 1
|
MCF-7 Tet-On cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: ACSL4-induced
|
Gene expression data
|
Sample_geo_accession | GSM1006130
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006130/suppl/GSM1006130_MCF_TET_A1.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006131 | GPL570 |
|
MCF-7 Tet-On ACSL4 2
|
MCF-7 Tet-On cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: ACSL4-induced
|
Gene expression data
|
Sample_geo_accession | GSM1006131
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006131/suppl/GSM1006131_MCF_TET_A2.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006132 | GPL570 |
|
MCF-7 Tet-On ACSL4 3
|
MCF-7 Tet-On cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: ACSL4-induced
|
Gene expression data
|
Sample_geo_accession | GSM1006132
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006132/suppl/GSM1006132_MCF_TET_A3.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006133 | GPL570 |
|
MCF-7 control 1
|
MCF-7 cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: control-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006133
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006133/suppl/GSM1006133_MCF_C1.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006134 | GPL570 |
|
MCF-7 control 2
|
MCF-7 cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: control-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006134
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006134/suppl/GSM1006134_MCF_C2.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006135 | GPL570 |
|
MCF-7 control 3
|
MCF-7 cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: control-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006135
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006135/suppl/GSM1006135_MCF_C3.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006136 | GPL570 |
|
MCF-7 ACSL4 1
|
MCF-7 cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: ACSL4-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006136
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006136/suppl/GSM1006136_MCF_A1.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006137 | GPL570 |
|
MCF-7 ACSL4 2
|
MCF-7 cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: ACSL4-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006137
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006137/suppl/GSM1006137_MCF_A2.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006138 | GPL570 |
|
MCF-7 ACSL4 3
|
MCF-7 cells
|
cell line: MCF-7
disease state: Estrogen receptor positive breast cancer
tissue: breast cancer
treatment: ACSL4-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006138
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006138/suppl/GSM1006138_MCF_A3.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006139 | GPL570 |
|
SKBr3 control 1
|
SKBr3 cells
|
cell line: SKBr3
disease state: HER2-positive breast cancer
tissue: breast cancer
treatment: control-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006139
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006139/suppl/GSM1006139_SK_C1.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006140 | GPL570 |
|
SKBr3 control 2
|
SKBr3 cells
|
cell line: SKBr3
disease state: HER2-positive breast cancer
tissue: breast cancer
treatment: control-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006140
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006140/suppl/GSM1006140_SK_C2.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006141 | GPL570 |
|
SKBr3 control 3
|
SKBr3 cells
|
cell line: SKBr3
disease state: HER2-positive breast cancer
tissue: breast cancer
treatment: control-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006141
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006141/suppl/GSM1006141_SK_C3.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006142 | GPL570 |
|
SKBr3 ACSL4 1
|
SKBr3 cells
|
cell line: SKBr3
disease state: HER2-positive breast cancer
tissue: breast cancer
treatment: ACSL4-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006142
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006142/suppl/GSM1006142_SK_A1.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006143 | GPL570 |
|
SKBr3 ACSL4 2
|
SKBr3 cells
|
cell line: SKBr3
disease state: HER2-positive breast cancer
tissue: breast cancer
treatment: ACSL4-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006143
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006143/suppl/GSM1006143_SK_A2.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
GSM1006144 | GPL570 |
|
SKBr3 ACSL4 3
|
SKBr3 cells
|
cell line: SKBr3
disease state: HER2-positive breast cancer
tissue: breast cancer
treatment: ACSL4-transfected
|
Gene expression data
|
Sample_geo_accession | GSM1006144
| Sample_status | Public on Sep 19 2012
| Sample_submission_date | Sep 18 2012
| Sample_last_update_date | Sep 19 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | For MCF_TET samples, cells were treated with either vehicle (MCF_TET_C) or doxycycline (1ug/ml) (MCF_TET_A) for 72 hours to induce ACSL4 expression. For MCF_ and SK_ samples, cells were stably transfected with a lentivirus-empty vector (MCF_C and SK_C) or lentivirus-ACSL4 vector (MCF_A and SK_A), and selscted clones from each transfection used as the source of RNA to be analyzed.
| Sample_growth_protocol_ch1 | Cell were grown in DMEM, high glucose, containing 10% fetal bovine serum and antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from cells using the RNAqueous-4PCR kit (AM1914, Ambion).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| Sample_hyb_protocol | labeled and fragmented cRNA hybridized to HG-U133_Plus_2 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent Genespring GX11 program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Jiri,,Zavadil
| Sample_contact_email | jiri.zavadil@med.nyu.edu
| Sample_contact_phone | 212-263-8048
| Sample_contact_department | Pathology
| Sample_contact_institute | NYU SoM
| Sample_contact_address |
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006144/suppl/GSM1006144_SK_A3.CEL.gz
| Sample_series_id | GSE40968
| Sample_data_row_count | 54675
| |
|
|
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Select GSMs and click on "Add groups" |
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