Search results for the GEO ID: GSE40971  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1006157 | GPL570 |
|
Pfeiffer vehicle, biological rep 1
|
Pfeiffer_DMSO
|
cell line: Pfeiffer
genotype/variation: EZH2 mutant
average gic50: 28 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
Pfeiffer_veh_R6.1_A
Gene expression data from Pfeiffer cell line treated with DMSO
|
| Sample_geo_accession | GSM1006157
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006157/suppl/GSM1006157_EA10008_149935_HG-U133_PLUS_2_R6-1.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006158 | GPL570 |
|
Pfeiffer vehicle, biological rep 2
|
Pfeiffer_DMSO
|
cell line: Pfeiffer
genotype/variation: EZH2 mutant
average gic50: 28 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
Pfeiffer_veh_R6.4_A
Gene expression data from Pfeiffer cell line treated with DMSO
|
| Sample_geo_accession | GSM1006158
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006158/suppl/GSM1006158_EA10008_149938_HG-U133_PLUS_2_R6-4.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006159 | GPL570 |
|
Pfeiffer GSK126, biological rep 1
|
Pfeiffer_GSK126
|
cell line: Pfeiffer
genotype/variation: EZH2 mutant
average gic50: 28 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
Pfeiffer_500nM_R6.2_B
Gene expression data from Pfeiffer cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006159
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006159/suppl/GSM1006159_EA10008_149936_HG-U133_PLUS_2_R6-2.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006160 | GPL570 |
|
Pfeiffer GSK126, biological rep 2
|
Pfeiffer_GSK126
|
cell line: Pfeiffer
genotype/variation: EZH2 mutant
average gic50: 28 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
Pfeiffer_500nM_R6.5_B
Gene expression data from Pfeiffer cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006160
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006160/suppl/GSM1006160_EA10008_149939_HG-U133_PLUS_2_R6-5.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006161 | GPL570 |
|
WSU-DLCL2 vehicle, biological rep 1
|
WSU-DLCL2_DMSO
|
cell line: WSU-DLCL2
genotype/variation: EZH2 mutant
average gic50: 134 nM nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
WSU.DLCL2_veh_R7.5_C
Gene expression data from WSU-DLCL2 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006161
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006161/suppl/GSM1006161_EA10008_223004_HG-U133_PLUS_2_R7-5.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006162 | GPL570 |
|
WSU-DLCL2 vehicle, biological rep 2
|
WSU-DLCL2_DMSO
|
cell line: WSU-DLCL2
genotype/variation: EZH2 mutant
average gic50: 134 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
WSU.DLCL2_veh_R7.6_C
Gene expression data from WSU-DLCL2 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006162
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006162/suppl/GSM1006162_EA10008_223005_HG-U133_PLUS_2_R7-6.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006163 | GPL570 |
|
WSU-DLCL2 GSK126, biological rep 1
|
WSU-DLCL2_GSK126
|
cell line: WSU-DLCL2
genotype/variation: EZH2 mutant
average gic50: 134 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
WSU.DLCL2_500nM_R7.7_D
Gene expression data from WSU-DLCL2cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006163
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006163/suppl/GSM1006163_EA10008_223006_HG-U133_PLUS_2_R7-7.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006164 | GPL570 |
|
WSU-DLCL2 GSK126, biological rep 2
|
WSU-DLCL2_GSK126
|
cell line: WSU-DLCL2
genotype/variation: EZH2 mutant
average gic50: 134 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
WSU.DLCL2_500nM_R7.8_D
Gene expression data from WSU-DLCL2 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006164
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006164/suppl/GSM1006164_EA10008_223007_HG-U133_PLUS_2_R7-8.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006165 | GPL570 |
|
KARPAS-422 vehicle, biological rep 1
|
KARPAS-422_DMSO
|
cell line: KARPAS-422
genotype/variation: EZH2 mutant
average gic50: 232 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
Karpas.422_veh_R6.37_E
Gene expression data from KARPAS-422 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006165
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006165/suppl/GSM1006165_EA10008_149971_HG-U133_PLUS_2_R6-37.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006166 | GPL570 |
|
KARPAS-422 vehicle, biological rep 2
|
KARPAS-422_DMSO
|
cell line: KARPAS-422
genotype/variation: EZH2 mutant
average gic50: 232 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
Karpas.422_veh_R6.40_E
Gene expression data from KARPAS-422 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006166
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006166/suppl/GSM1006166_EA10008_149974_HG-U133_PLUS_2_R6-40.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006167 | GPL570 |
|
KARPAS-422 GSK126, biological rep 1
|
KARPAS-422_GSK126
|
cell line: KARPAS-422
genotype/variation: EZH2 mutant
average gic50: 232 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
Karpas.422_500nM_R6.38_F
Gene expression data from KARPAS-422 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006167
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006167/suppl/GSM1006167_EA10008_149972_HG-U133_PLUS_2_R6-38.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006168 | GPL570 |
|
KARPAS-422 GSK126, biological rep 2
|
KARPAS-422_GSK126
|
cell line: KARPAS-422
genotype/variation: EZH2 mutant
average gic50: 232 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
Karpas.422_500nM_R6.41_F
Gene expression data from KARPAS-422cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006168
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006168/suppl/GSM1006168_EA10008_149975_HG-U133_PLUS_2_R6-41.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006169 | GPL570 |
|
SU-DHL-10 vehicle, biological rep 1
|
SU-DHL-10_DMSO
|
cell line: SU-DHL-10
genotype/variation: EZH2 mutant
average gic50: 448 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
SU.DHL.10_veh_R7.33_G
Gene expression data from SU-DHL-10 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006169
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006169/suppl/GSM1006169_EA10008_223032_HG-U133_PLUS_2_R7-33.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006170 | GPL570 |
|
SU-DHL-10 vehicle, biological rep 2
|
SU-DHL-10_DMSO
|
cell line: SU-DHL-10
genotype/variation: EZH2 mutant
average gic50: 448 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
SU.DHL.10_veh_R7.34_G
Gene expression data from SU-DHL-10 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006170
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006170/suppl/GSM1006170_EA10008_223033_HG-U133_PLUS_2_R7-34.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006171 | GPL570 |
|
SU-DHL-10 GSK126, biological rep 1
|
SU-DHL-10_GSK126
|
cell line: SU-DHL-10
genotype/variation: EZH2 mutant
average gic50: 448 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
SU.DHL.10_500nM_R7.35_H
Gene expression data from SU-DHL-10 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006171
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006171/suppl/GSM1006171_EA10008_223034_HG-U133_PLUS_2_R7-35.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006172 | GPL570 |
|
SU-DHL-10 GSK126, biological rep 2
|
SU-DHL-10_GSK126
|
cell line: SU-DHL-10
genotype/variation: EZH2 mutant
average gic50: 448 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
SU.DHL.10_500nM_R7.36_H
Gene expression data from SU-DHL-10 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006172
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006172/suppl/GSM1006172_EA10008_223035_HG-U133_PLUS_2_R7-36.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006173 | GPL570 |
|
HT vehicle, biological rep 1
|
HT_DMSO
|
cell line: HT
genotype/variation: EZH2 wild type
average gic50: 516 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
HT _veh_R7.25_I
Gene expression data from HT cell line treated with DMSO
|
| Sample_geo_accession | GSM1006173
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006173/suppl/GSM1006173_EA10008_223024_HG-U133_PLUS_2_R7-25.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006174 | GPL570 |
|
HT vehicle, biological rep 2
|
HT_DMSO
|
cell line: HT
genotype/variation: EZH2 wild type
average gic50: 516 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
HT _veh_R7.26_I
Gene expression data from HT cell line treated with DMSO
|
| Sample_geo_accession | GSM1006174
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006174/suppl/GSM1006174_EA10008_223025_HG-U133_PLUS_2_R7-26.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006175 | GPL570 |
|
HT GSK126, biological rep 1
|
HT_GSK126
|
cell line: HT
genotype/variation: EZH2 wild type
average gic50: 516 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
HT _500nM_R7.27_J
Gene expression data from HT cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006175
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006175/suppl/GSM1006175_EA10008_223026_HG-U133_PLUS_2_R7-27.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006176 | GPL570 |
|
HT GSK126, biological rep 2
|
HT_GSK126
|
cell line: HT
genotype/variation: EZH2 wild type
average gic50: 516 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
HT _500nM_R7.28_J
Gene expression data from HT cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006176
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006176/suppl/GSM1006176_EA10008_223027_HG-U133_PLUS_2_R7-28.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006177 | GPL570 |
|
SU-DHL-6 vehicle, biological rep 1
|
SU-DHL-6_DMSO
|
cell line: SU-DHL-6
genotype/variation: EZH2 mutant
average gic50: 582 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
SU.DHL.6_veh_R8.21_K
Gene expression data from SU-DHL-6 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006177
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006177/suppl/GSM1006177_EA10008_232950_HG-U133_PLUS_2_R8-21.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006178 | GPL570 |
|
SU-DHL-6 vehicle, biological rep 2
|
SU-DHL-6_DMSO
|
cell line: SU-DHL-6
genotype/variation: EZH2 mutant
average gic50: 582 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
SU.DHL.6_veh_R8.22_K
Gene expression data from SU-DHL-6 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006178
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006178/suppl/GSM1006178_EA10008_232951_HG-U133_PLUS_2_R8-22.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006179 | GPL570 |
|
SU-DHL-6 GSK126, biological rep 1
|
SU-DHL-6_GSK126
|
cell line: SU-DHL-6
genotype/variation: EZH2 mutant
average gic50: 582 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
SU.DHL.6_500nM_R8.23_L
Gene expression data from SU-DHL-6 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006179
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006179/suppl/GSM1006179_EA10008_232952_HG-U133_PLUS_2_R8-23.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006180 | GPL570 |
|
SU-DHL-6 GSK126, biological rep 2
|
SU-DHL-6_GSK126
|
cell line: SU-DHL-6
genotype/variation: EZH2 mutant
average gic50: 582 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
SU.DHL.6_500nM_R8.24_L
Gene expression data from SU-DHL-6 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006180
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006180/suppl/GSM1006180_EA10008_232953_HG-U133_PLUS_2_R8-24.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006181 | GPL570 |
|
DB vehicle, biological rep 1
|
DB_DMSO
|
cell line: DB
genotype/variation: EZH2 mutant
average gic50: 861 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
DB_veh_R7.1_M
Gene expression data from DB cell line treated with DMSO
|
| Sample_geo_accession | GSM1006181
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006181/suppl/GSM1006181_EA10008_223000_HG-U133_PLUS_2_R7-1.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006182 | GPL570 |
|
DB vehicle, biological rep 2
|
DB_DMSO
|
cell line: DB
genotype/variation: EZH2 mutant
average gic50: 861 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
DB_veh_R7.2_M
Gene expression data from DB cell line treated with DMSO
|
| Sample_geo_accession | GSM1006182
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006182/suppl/GSM1006182_EA10008_223001_HG-U133_PLUS_2_R7-2.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006183 | GPL570 |
|
DB GSK126, biological rep 1
|
DB_GSK126
|
cell line: DB
genotype/variation: EZH2 mutant
average gic50: 861 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
DB_500nM_R7.3_N
Gene expression data from DB cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006183
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006183/suppl/GSM1006183_EA10008_223002_HG-U133_PLUS_2_R7-3.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006184 | GPL570 |
|
DB GSK126, biological rep 2
|
DB_GSK126
|
cell line: DB
genotype/variation: EZH2 mutant
average gic50: 861 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
DB_500nM_R7.4_N
Gene expression data from DB cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006184
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006184/suppl/GSM1006184_EA10008_223003_HG-U133_PLUS_2_R7-4.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006185 | GPL570 |
|
OCI-LY-19 vehicle, biological rep 1
|
OCI-LY-19_DMSO
|
cell line: OCI-LY-19
genotype/variation: EZH2 wild type
average gic50: 1019 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
OCI.LY.19_veh_R8.9_O
Gene expression data from OCI-LY-19 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006185
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006185/suppl/GSM1006185_EA10008_232938_HG-U133_PLUS_2_R8-9.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006186 | GPL570 |
|
OCI-LY-19 vehicle, biological rep 2
|
OCI-LY-19_DMSO
|
cell line: OCI-LY-19
genotype/variation: EZH2 wild type
average gic50: 1019 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
OCI.LY.19_veh_R8.10_O
Gene expression data from OCI-LY-19 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006186
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006186/suppl/GSM1006186_EA10008_232939_HG-U133_PLUS_2_R8-10.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006187 | GPL570 |
|
OCI-LY-19 GSK126, biological rep 1
|
OCI-LY-19_GSK126
|
cell line: OCI-LY-19
genotype/variation: EZH2 wild type
average gic50: 1019 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
OCI.LY.19_500nM_R8.11_P
Gene expression data from OCI-LY-19 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006187
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006187/suppl/GSM1006187_EA10008_232940_HG-U133_PLUS_2_R8-11.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006188 | GPL570 |
|
OCI-LY-19 GSK126, biological rep 2
|
OCI-LY-19_GSK126
|
cell line: OCI-LY-19
genotype/variation: EZH2 wild type
average gic50: 1019 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
OCI.LY.19_500nM_R8.12_P
Gene expression data from OCI-LY-19 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006188
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006188/suppl/GSM1006188_EA10008_232941_HG-U133_PLUS_2_R8-12.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006189 | GPL570 |
|
SU-DHL-4 vehicle, biological rep 1
|
SU-DHL-4_DMSO
|
cell line: SU-DHL-4
genotype/variation: EZH2 mutant
average gic50 (nm): 4828
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
SU.DHL.4_veh_R7.13_Q
Gene expression data from SU-DHL-4 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006189
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006189/suppl/GSM1006189_EA10008_223012_HG-U133_PLUS_2_R7-13.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006190 | GPL570 |
|
SU-DHL-4 vehicle, biological rep 2
|
SU-DHL-4_DMSO
|
cell line: SU-DHL-4
genotype/variation: EZH2 mutant
average gic50 (nm): 4828
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
SU.DHL.4_veh_R7.14_Q
Gene expression data from SU-DHL-4 cell line treated with DMSO
|
| Sample_geo_accession | GSM1006190
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006190/suppl/GSM1006190_EA10008_223013_HG-U133_PLUS_2_R7-14.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006191 | GPL570 |
|
SU-DHL-4 GSK126, biological rep 1
|
SU-DHL-4_GSK126
|
cell line: SU-DHL-4
genotype/variation: EZH2 mutant
average gic50 (nm): 4828
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
SU.DHL.4_500nM_R7.15_R
Gene expression data from SU-DHL-4 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006191
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006191/suppl/GSM1006191_EA10008_223014_HG-U133_PLUS_2_R7-15.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006192 | GPL570 |
|
SU-DHL-4 GSK126, biological rep 2
|
SU-DHL-4_GSK126
|
cell line: SU-DHL-4
genotype/variation: EZH2 mutant
average gic50 (nm): 4828
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
SU.DHL.4_500nM_R7.16_R
Gene expression data from SU-DHL-4 cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006192
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006192/suppl/GSM1006192_EA10008_223015_HG-U133_PLUS_2_R7-16.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006193 | GPL570 |
|
Toledo vehicle, biological rep 1
|
Toledo_DMSO
|
cell line: Toledo
genotype/variation: EZH2 wild type
average gic50: 13786 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
Toledo_veh_R6.7_S
Gene expression data from Toledo cell line treated with DMSO
|
| Sample_geo_accession | GSM1006193
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006193/suppl/GSM1006193_EA10008_149941_HG-U133_PLUS_2_R6-7.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006194 | GPL570 |
|
Toledo vehicle, biological rep 2
|
Toledo_DMSO
|
cell line: Toledo
genotype/variation: EZH2 wild type
average gic50: 13786 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: DMSO (72h)
|
Toledo_veh_R6.10_S
Gene expression data from Toledo cell line treated with DMSO
|
| Sample_geo_accession | GSM1006194
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006194/suppl/GSM1006194_EA10008_149944_HG-U133_PLUS_2_R6-10.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006195 | GPL570 |
|
Toledo GSK126, biological rep 1
|
Toledo_GSK126
|
cell line: Toledo
genotype/variation: EZH2 wild type
average gic50: 13786 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
Toledo_500nM_R6.8_T
Gene expression data from Toledo cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006195
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006195/suppl/GSM1006195_EA10008_149942_HG-U133_PLUS_2_R6-8.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
|
GSM1006196 | GPL570 |
|
Toledo GSK126, biological rep 2
|
Toledo_GSK126
|
cell line: Toledo
genotype/variation: EZH2 wild type
average gic50: 13786 nM
tissue type: DLBCL (diffuse large B-cell lymphoma)
treatment: GSK126 (500nM; 72h)
|
Toledo_500nM_R6.11_T
Gene expression data from Toledo cell line treated with 500nM GSK126 treatment
|
| Sample_geo_accession | GSM1006196
| | Sample_status | Public on Oct 12 2012
| | Sample_submission_date | Sep 18 2012
| | Sample_last_update_date | Oct 12 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2x10^5 cells were seeded in 3 ml cell culture media into 6-well dishes and allowed to equilibrate overnight at 37 degrees Celcius and 5% CO2. The following day, DMSO or GSK126 were added to final concentrations of 0.1% DMSO and/or 500 nM GSK126. After 72 hours, cells were harvested by centrifugation, lysed with 750 ul Trizol reagent, and frozen at -80 degrees Celcius.
| | Sample_growth_protocol_ch1 | Cells were grown as recommended by ATCC or DSMZ.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extracted with Trizol reagent and purified per Trizol protocol; RNA further purified using RNeasy kit columns
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | According to manufacturer's instructions
| | Sample_hyb_protocol | According to manufacturer's instructions
| | Sample_scan_protocol | According to manufacturer's instructions
| | Sample_data_processing | Data was analyzed using MAS (Microarray Suite) v. 5.0 algorithm using defulat settings and global scaling as the normalization method. The trimmed mean target intensity of each array was set to 500
| | Sample_platform_id | GPL570
| | Sample_contact_name | Gopinath,,Ganji
| | Sample_contact_email | gopi@gsk.com
| | Sample_contact_institute | GSK
| | Sample_contact_address | 1250 South Collegeville Road
| | Sample_contact_city | Collegeville
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19426
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006196/suppl/GSM1006196_EA10008_149945_HG-U133_PLUS_2_R6-11.CEL.gz
| | Sample_series_id | GSE40971
| | Sample_series_id | GSE40972
| | Sample_data_row_count | 54675
| | |
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