Result

Search results for the GEO ID: GSE40988

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1006383

GPL570

cell line L-428, vector control

Hodgkin’s lymphoma cell line L-428, transfected with control vector pcDNA6.2-GW/EmGFP-miR-neg

cell type: Hodgkin’s lymphoma cell line cell line: L-428 genotype/variation: transfected with control vector pcDNA6.2-GW/EmGFP-miR-neg

Gene expression data from established Hodgkin's lymphoma cell line

Sample_geo_accession	GSM1006383
Sample_status	Public on Dec 21 2012
Sample_submission_date	Sep 19 2012
Sample_last_update_date	Dec 21 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were transfected with empty control vector pcDNA6.2-GW/EmGFP-miR-neg or with vector pcDNA6.2-GW/EmGFP-miR-PRAME
Sample_growth_protocol_ch1	Established Hodgkin's lymphoma cells were grown under standard conditions.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 10 ug total RNA.
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on HG_U133Plus2.0 Microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000
Sample_data_processing	The data were analyzed using the MAS5.0 algorithm and Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500. We used Expression Console version 1.1 (Affymetrix) for the scaling of the data.
Sample_platform_id	GPL570
Sample_contact_name	Martin,Sebastian,Staege
Sample_contact_email	martin.staege@medizin.uni-halle.de
Sample_contact_phone	+49-345-5577280
Sample_contact_laboratory	Children's Cancer Reserach Center
Sample_contact_department	Universitätsklinik u. Poliklinik für Kinder- u. Jugendmedizin
Sample_contact_institute	Martin-Luther-University Halle-Wittenberg
Sample_contact_address	Ernst-Grube-Str. 40
Sample_contact_city	Halle (Saale)
Sample_contact_state	Sachsen-Anhalt
Sample_contact_zip/postal_code	06120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006383/suppl/GSM1006383_L-428_miR_LV.CEL.gz
Sample_series_id	GSE40988
Sample_data_row_count	54675

GSM1006384

GPL570

cell line L-428, knock-down of PRAME

Hodgkin’s lymphoma cell line L-428, transfected with vector pcDNA6.2-GW/EmGFP-miR-PRAME

cell type: Hodgkin’s lymphoma cell line cell line: L-428 genotype/variation: transfected with vector pcDNA6.2-GW/EmGFP-miR-PRAME

Gene expression data from established Hodgkin's lymphoma cell line after knock-down of PRAME

Sample_geo_accession	GSM1006384
Sample_status	Public on Dec 21 2012
Sample_submission_date	Sep 19 2012
Sample_last_update_date	Dec 21 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Cells were transfected with empty control vector pcDNA6.2-GW/EmGFP-miR-neg or with vector pcDNA6.2-GW/EmGFP-miR-PRAME
Sample_growth_protocol_ch1	Established Hodgkin's lymphoma cells were grown under standard conditions.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 10 ug total RNA.
Sample_hyb_protocol	Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on HG_U133Plus2.0 Microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000
Sample_data_processing	The data were analyzed using the MAS5.0 algorithm and Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500. We used Expression Console version 1.1 (Affymetrix) for the scaling of the data.
Sample_platform_id	GPL570
Sample_contact_name	Martin,Sebastian,Staege
Sample_contact_email	martin.staege@medizin.uni-halle.de
Sample_contact_phone	+49-345-5577280
Sample_contact_laboratory	Children's Cancer Reserach Center
Sample_contact_department	Universitätsklinik u. Poliklinik für Kinder- u. Jugendmedizin
Sample_contact_institute	Martin-Luther-University Halle-Wittenberg
Sample_contact_address	Ernst-Grube-Str. 40
Sample_contact_city	Halle (Saale)
Sample_contact_state	Sachsen-Anhalt
Sample_contact_zip/postal_code	06120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006384/suppl/GSM1006384_L-428_miR_PRAME.CEL.gz
Sample_series_id	GSE40988
Sample_data_row_count	54675

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