Search results for the GEO ID: GSE41005  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1006698 | GPL1261 |
|
HSF1+/+ T cells at 37oC replicate #1
|
HSF1+/+ T cells activated at 37oC
|
cell type: T cell
treatment: 37C
genotype: HSF1+/+
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1+/+ T cells activated at 37oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006698
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006698/suppl/GSM1006698_pmHS1-37.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006698/suppl/GSM1006698_pmHS1-37.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006699 | GPL1261 |
|
HSF1+/+ T cells at 37oC replicate #2
|
HSF1+/+ T cells activated at 37oC
|
cell type: T cell
treatment: 37C
genotype: HSF1+/+
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1+/+ T cells activated at 37oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006699
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006699/suppl/GSM1006699_pmHS2-37.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006699/suppl/GSM1006699_pmHS2-37.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006700 | GPL1261 |
|
HSF1-/- T cells at 37oC replicate #1
|
HSF1-/- T cells activated at 37oC
|
cell type: T cell
treatment: 37C
genotype: HSF1-/-
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1-/- T cells activated at 37oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006700
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006700/suppl/GSM1006700_pmHS3-37.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006700/suppl/GSM1006700_pmHS3-37.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006701 | GPL1261 |
|
HSF1-/- T cells at 37oC replicate #2
|
HSF1-/- T cells activated at 37oC
|
cell type: T cell
treatment: 37C
genotype: HSF1-/-
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1-/- T cells activated at 37oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006701
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006701/suppl/GSM1006701_pmHS4-37.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006701/suppl/GSM1006701_pmHS4-37.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006702 | GPL1261 |
|
HSF1+/+ T cells at 40oC replicate #1
|
HSF1+/+ T cells activated at 40oC
|
cell type: T cell
treatment: 40C
genotype: HSF1+/+
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1+/+ T cells activated at 40oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006702
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006702/suppl/GSM1006702_pmHS1-40.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006702/suppl/GSM1006702_pmHS1-40.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006703 | GPL1261 |
|
HSF1+/+ T cells at 40oC replicate #2
|
HSF1+/+ T cells activated at 40oC
|
cell type: T cell
treatment: 40C
genotype: HSF1+/+
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1+/+ T cells activated at 40oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006703
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006703/suppl/GSM1006703_pmHS2-40.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006703/suppl/GSM1006703_pmHS2-40.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006704 | GPL1261 |
|
HSF1-/- T cells at 40oC replicate #1
|
HSF1-/- T cells activated at 40oC
|
cell type: T cell
treatment: 40C
genotype: HSF1-/-
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1-/- T cells activated at 40oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006704
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006704/suppl/GSM1006704_pmHS3-40.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006704/suppl/GSM1006704_pmHS3-40.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
|
GSM1006705 | GPL1261 |
|
HSF1-/- T cells at 40oC replicate #2
|
HSF1-/- T cells activated at 40oC
|
cell type: T cell
treatment: 40C
genotype: HSF1-/-
genetic background: mixed 129Sv.BALB/c background
|
5hr activation with plate bound anti-CD3
Gene expression data from HSF1-/- T cells activated at 40oC for 5h with plate bound anti-CD3.
|
| Sample_geo_accession | GSM1006705
| | Sample_status | Public on Sep 20 2012
| | Sample_submission_date | Sep 19 2012
| | Sample_last_update_date | Sep 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | T cells were activated with plate bound anti-CD3
| | Sample_growth_protocol_ch1 | Purified T cells were cultured in RPMI 1640 medium for 5h at 37oC or 40oC and at 5% CO2
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA from activated T cells were isolated using RNA easy Kit according to manufacturer's protocol. The RNA quantification and quality was performed using a Model 2100 bioanalyzer.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared by transcribing 2.5 μg of Total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Following fragmentation, 20μg of cRNA were hybridized for 16h at 45C on whole mouse Affymetrix 430-2.0 Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as a normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Statistical analysis was performed using two-way ANOVA and SAS software.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jerold,G,Woodward
| | Sample_contact_email | jwood1@uky.edu
| | Sample_contact_phone | 859-323-5584
| | Sample_contact_fax | 859-323-8994
| | Sample_contact_laboratory | Immunology
| | Sample_contact_department | Microbiology, Immunology & Molecular Genetics
| | Sample_contact_institute | University of Kentucky
| | Sample_contact_address | 800 Rose St. MS-415
| | Sample_contact_city | Lexington
| | Sample_contact_state | ky
| | Sample_contact_zip/postal_code | 40536-0298
| | Sample_contact_country | USA
| | Sample_contact_web_link | http://www.mc.uky.edu/microbiology/woodward.asp
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006705/suppl/GSM1006705_pmHS4-40.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1006nnn/GSM1006705/suppl/GSM1006705_pmHS4-40.CHP.gz
| | Sample_series_id | GSE41005
| | Sample_data_row_count | 45101
| | |
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