Search results for the GEO ID: GSE41085 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1008419 | GPL1261 |
|
2 hours single frequency THz irradiation, biological rep1
|
Mouse mesenchymal stem cells
|
cell type: mesenchymal stem cells
thz irradiation conditions: 2 hours single frequency THz irradiation
|
|
Sample_geo_accession | GSM1008419
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | Sep 21 2012
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells are irradiated for 2 hours with a far-IR optically pumped molecular gas continuous wave THz laser source (Edinburgh Instruments FIRL 100). All experiments with this laser were conducted at frequency 2.52 THz (lambda=118.8 um).
| Sample_growth_protocol_ch1 | Mouse mesenchymal stem cells (MSCs) (ScienCell Research Laboratories, CA 92011, Catalog Number M7500) were cultured on tissue culture treated plastic T-75 flasks (Corning). Once the cells reached 80-90% confluence, the cells were sub-cultured in supplemented medium (95% α-MEM, 5% FBS, and antibiotics) for THz irradiation treatment. Roziglitazone (1 µM), insulin (5 µg/ml), 3-Isobutyl-1-methylxanthine (100 µM), and dexamethasone (1 µM), were added to the medium again 48 hours prior to irradiation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cellular RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using an oligo dT-primer containing the T7 RNApolymerase promoter site and the One-Cycle Target Labeling Kit (Affymetrix). Biotin-labeled cRNA was purified and the absorbance measured at 260 nm to determine yield (NanoDrop).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | The raw microarray .CEL files were analyzed using R and Bioconductor. More specifically, the robust multi-array (RMA) method included in the affy package was used for preprocessing, background correction, and normalization of the raw data. Probes without Entrez identifiers or gene symbols as well as probes with small standard deviation across samples were filtered out from the analysis. Further, features mapping to the same Entrez identifier were consolidated by leaving the one with largest value and, finally, probes with standard deviation less than 0.5 across samples were filtered out from the analysis. Differentially expressed genes were identified using the Bioconductor limma package and ranked by calculating the B statistics for each gene. Gene annotation was performed using the Bioconductor mouse4302.db package, while the hypergeometric test of the Bioconductor’s GOstats package was used for identifying overrepresentation of differentially expressed genes in gene ontology terms.
| Sample_platform_id | GPL1261
| Sample_contact_name | Boian,S,Alexandrov
| Sample_contact_email | boian@lanl.gov
| Sample_contact_department | Theoretical Division
| Sample_contact_institute | LANL
| Sample_contact_address | Bikini Atoll Road
| Sample_contact_city | Los Alamos
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87545
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008419/suppl/GSM1008419_13_Mouse430_2.CEL.gz
| Sample_series_id | GSE41085
| Sample_series_id | GSE41106
| Sample_data_row_count | 45101
| |
|
GSM1008420 | GPL1261 |
|
Control for 2 hours single frequency THz irradiation, biological rep1
|
Mouse mesenchymal stem cells
|
cell type: mesenchymal stem cells
thz irradiation conditions: none
|
|
Sample_geo_accession | GSM1008420
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | Sep 21 2012
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells are irradiated for 2 hours with a far-IR optically pumped molecular gas continuous wave THz laser source (Edinburgh Instruments FIRL 100). All experiments with this laser were conducted at frequency 2.52 THz (lambda=118.8 um).
| Sample_growth_protocol_ch1 | Mouse mesenchymal stem cells (MSCs) (ScienCell Research Laboratories, CA 92011, Catalog Number M7500) were cultured on tissue culture treated plastic T-75 flasks (Corning). Once the cells reached 80-90% confluence, the cells were sub-cultured in supplemented medium (95% α-MEM, 5% FBS, and antibiotics) for THz irradiation treatment. Roziglitazone (1 µM), insulin (5 µg/ml), 3-Isobutyl-1-methylxanthine (100 µM), and dexamethasone (1 µM), were added to the medium again 48 hours prior to irradiation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cellular RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using an oligo dT-primer containing the T7 RNApolymerase promoter site and the One-Cycle Target Labeling Kit (Affymetrix). Biotin-labeled cRNA was purified and the absorbance measured at 260 nm to determine yield (NanoDrop).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | The raw microarray .CEL files were analyzed using R and Bioconductor. More specifically, the robust multi-array (RMA) method included in the affy package was used for preprocessing, background correction, and normalization of the raw data. Probes without Entrez identifiers or gene symbols as well as probes with small standard deviation across samples were filtered out from the analysis. Further, features mapping to the same Entrez identifier were consolidated by leaving the one with largest value and, finally, probes with standard deviation less than 0.5 across samples were filtered out from the analysis. Differentially expressed genes were identified using the Bioconductor limma package and ranked by calculating the B statistics for each gene. Gene annotation was performed using the Bioconductor mouse4302.db package, while the hypergeometric test of the Bioconductor’s GOstats package was used for identifying overrepresentation of differentially expressed genes in gene ontology terms.
| Sample_platform_id | GPL1261
| Sample_contact_name | Boian,S,Alexandrov
| Sample_contact_email | boian@lanl.gov
| Sample_contact_department | Theoretical Division
| Sample_contact_institute | LANL
| Sample_contact_address | Bikini Atoll Road
| Sample_contact_city | Los Alamos
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87545
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008420/suppl/GSM1008420_14_Mouse430_2.CEL.gz
| Sample_series_id | GSE41085
| Sample_series_id | GSE41106
| Sample_data_row_count | 45101
| |
|
GSM1008421 | GPL1261 |
|
2 hours single frequency THz irradiation, biological rep2
|
Mouse mesenchymal stem cells
|
cell type: mesenchymal stem cells
thz irradiation conditions: 2 hours single frequency THz irradiation
|
|
Sample_geo_accession | GSM1008421
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | Sep 21 2012
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells are irradiated for 2 hours with a far-IR optically pumped molecular gas continuous wave THz laser source (Edinburgh Instruments FIRL 100). All experiments with this laser were conducted at frequency 2.52 THz (lambda=118.8 um).
| Sample_growth_protocol_ch1 | Mouse mesenchymal stem cells (MSCs) (ScienCell Research Laboratories, CA 92011, Catalog Number M7500) were cultured on tissue culture treated plastic T-75 flasks (Corning). Once the cells reached 80-90% confluence, the cells were sub-cultured in supplemented medium (95% α-MEM, 5% FBS, and antibiotics) for THz irradiation treatment. Roziglitazone (1 µM), insulin (5 µg/ml), 3-Isobutyl-1-methylxanthine (100 µM), and dexamethasone (1 µM), were added to the medium again 48 hours prior to irradiation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cellular RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using an oligo dT-primer containing the T7 RNApolymerase promoter site and the One-Cycle Target Labeling Kit (Affymetrix). Biotin-labeled cRNA was purified and the absorbance measured at 260 nm to determine yield (NanoDrop).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | The raw microarray .CEL files were analyzed using R and Bioconductor. More specifically, the robust multi-array (RMA) method included in the affy package was used for preprocessing, background correction, and normalization of the raw data. Probes without Entrez identifiers or gene symbols as well as probes with small standard deviation across samples were filtered out from the analysis. Further, features mapping to the same Entrez identifier were consolidated by leaving the one with largest value and, finally, probes with standard deviation less than 0.5 across samples were filtered out from the analysis. Differentially expressed genes were identified using the Bioconductor limma package and ranked by calculating the B statistics for each gene. Gene annotation was performed using the Bioconductor mouse4302.db package, while the hypergeometric test of the Bioconductor’s GOstats package was used for identifying overrepresentation of differentially expressed genes in gene ontology terms.
| Sample_platform_id | GPL1261
| Sample_contact_name | Boian,S,Alexandrov
| Sample_contact_email | boian@lanl.gov
| Sample_contact_department | Theoretical Division
| Sample_contact_institute | LANL
| Sample_contact_address | Bikini Atoll Road
| Sample_contact_city | Los Alamos
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87545
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008421/suppl/GSM1008421_15_Mouse430_2.CEL.gz
| Sample_series_id | GSE41085
| Sample_series_id | GSE41106
| Sample_data_row_count | 45101
| |
|
GSM1008422 | GPL1261 |
|
Control for 2 hours single frequency THz irradiation, biological rep2
|
Mouse mesenchymal stem cells
|
cell type: mesenchymal stem cells
thz irradiation conditions: none
|
|
Sample_geo_accession | GSM1008422
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | Sep 21 2012
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells are irradiated for 2 hours with a far-IR optically pumped molecular gas continuous wave THz laser source (Edinburgh Instruments FIRL 100). All experiments with this laser were conducted at frequency 2.52 THz (lambda=118.8 um).
| Sample_growth_protocol_ch1 | Mouse mesenchymal stem cells (MSCs) (ScienCell Research Laboratories, CA 92011, Catalog Number M7500) were cultured on tissue culture treated plastic T-75 flasks (Corning). Once the cells reached 80-90% confluence, the cells were sub-cultured in supplemented medium (95% α-MEM, 5% FBS, and antibiotics) for THz irradiation treatment. Roziglitazone (1 µM), insulin (5 µg/ml), 3-Isobutyl-1-methylxanthine (100 µM), and dexamethasone (1 µM), were added to the medium again 48 hours prior to irradiation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cellular RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using an oligo dT-primer containing the T7 RNApolymerase promoter site and the One-Cycle Target Labeling Kit (Affymetrix). Biotin-labeled cRNA was purified and the absorbance measured at 260 nm to determine yield (NanoDrop).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | The raw microarray .CEL files were analyzed using R and Bioconductor. More specifically, the robust multi-array (RMA) method included in the affy package was used for preprocessing, background correction, and normalization of the raw data. Probes without Entrez identifiers or gene symbols as well as probes with small standard deviation across samples were filtered out from the analysis. Further, features mapping to the same Entrez identifier were consolidated by leaving the one with largest value and, finally, probes with standard deviation less than 0.5 across samples were filtered out from the analysis. Differentially expressed genes were identified using the Bioconductor limma package and ranked by calculating the B statistics for each gene. Gene annotation was performed using the Bioconductor mouse4302.db package, while the hypergeometric test of the Bioconductor’s GOstats package was used for identifying overrepresentation of differentially expressed genes in gene ontology terms.
| Sample_platform_id | GPL1261
| Sample_contact_name | Boian,S,Alexandrov
| Sample_contact_email | boian@lanl.gov
| Sample_contact_department | Theoretical Division
| Sample_contact_institute | LANL
| Sample_contact_address | Bikini Atoll Road
| Sample_contact_city | Los Alamos
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87545
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008422/suppl/GSM1008422_16_Mouse430_2.CEL.gz
| Sample_series_id | GSE41085
| Sample_series_id | GSE41106
| Sample_data_row_count | 45101
| |
|
GSM1008423 | GPL1261 |
|
2 hours single frequency THz irradiation, biological rep3
|
Mouse mesenchymal stem cells
|
cell type: mesenchymal stem cells
thz irradiation conditions: 2 hours single frequency THz irradiation
|
|
Sample_geo_accession | GSM1008423
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | Sep 21 2012
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells are irradiated for 2 hours with a far-IR optically pumped molecular gas continuous wave THz laser source (Edinburgh Instruments FIRL 100). All experiments with this laser were conducted at frequency 2.52 THz (lambda=118.8 um).
| Sample_growth_protocol_ch1 | Mouse mesenchymal stem cells (MSCs) (ScienCell Research Laboratories, CA 92011, Catalog Number M7500) were cultured on tissue culture treated plastic T-75 flasks (Corning). Once the cells reached 80-90% confluence, the cells were sub-cultured in supplemented medium (95% α-MEM, 5% FBS, and antibiotics) for THz irradiation treatment. Roziglitazone (1 µM), insulin (5 µg/ml), 3-Isobutyl-1-methylxanthine (100 µM), and dexamethasone (1 µM), were added to the medium again 48 hours prior to irradiation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cellular RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using an oligo dT-primer containing the T7 RNApolymerase promoter site and the One-Cycle Target Labeling Kit (Affymetrix). Biotin-labeled cRNA was purified and the absorbance measured at 260 nm to determine yield (NanoDrop).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | The raw microarray .CEL files were analyzed using R and Bioconductor. More specifically, the robust multi-array (RMA) method included in the affy package was used for preprocessing, background correction, and normalization of the raw data. Probes without Entrez identifiers or gene symbols as well as probes with small standard deviation across samples were filtered out from the analysis. Further, features mapping to the same Entrez identifier were consolidated by leaving the one with largest value and, finally, probes with standard deviation less than 0.5 across samples were filtered out from the analysis. Differentially expressed genes were identified using the Bioconductor limma package and ranked by calculating the B statistics for each gene. Gene annotation was performed using the Bioconductor mouse4302.db package, while the hypergeometric test of the Bioconductor’s GOstats package was used for identifying overrepresentation of differentially expressed genes in gene ontology terms.
| Sample_platform_id | GPL1261
| Sample_contact_name | Boian,S,Alexandrov
| Sample_contact_email | boian@lanl.gov
| Sample_contact_department | Theoretical Division
| Sample_contact_institute | LANL
| Sample_contact_address | Bikini Atoll Road
| Sample_contact_city | Los Alamos
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87545
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008423/suppl/GSM1008423_17_Mouse430_2.CEL.gz
| Sample_series_id | GSE41085
| Sample_series_id | GSE41106
| Sample_data_row_count | 45101
| |
|
GSM1008424 | GPL1261 |
|
Control for 2 hours single frequency THz irradiation, biological rep3
|
Mouse mesenchymal stem cells
|
cell type: mesenchymal stem cells
thz irradiation conditions: none
|
|
Sample_geo_accession | GSM1008424
| Sample_status | Public on Jul 01 2013
| Sample_submission_date | Sep 21 2012
| Sample_last_update_date | Jul 01 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Cells are irradiated for 2 hours with a far-IR optically pumped molecular gas continuous wave THz laser source (Edinburgh Instruments FIRL 100). All experiments with this laser were conducted at frequency 2.52 THz (lambda=118.8 um).
| Sample_growth_protocol_ch1 | Mouse mesenchymal stem cells (MSCs) (ScienCell Research Laboratories, CA 92011, Catalog Number M7500) were cultured on tissue culture treated plastic T-75 flasks (Corning). Once the cells reached 80-90% confluence, the cells were sub-cultured in supplemented medium (95% α-MEM, 5% FBS, and antibiotics) for THz irradiation treatment. Roziglitazone (1 µM), insulin (5 µg/ml), 3-Isobutyl-1-methylxanthine (100 µM), and dexamethasone (1 µM), were added to the medium again 48 hours prior to irradiation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cellular RNA was extracted using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA using an oligo dT-primer containing the T7 RNApolymerase promoter site and the One-Cycle Target Labeling Kit (Affymetrix). Biotin-labeled cRNA was purified and the absorbance measured at 260 nm to determine yield (NanoDrop).
| Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
| Sample_data_processing | The raw microarray .CEL files were analyzed using R and Bioconductor. More specifically, the robust multi-array (RMA) method included in the affy package was used for preprocessing, background correction, and normalization of the raw data. Probes without Entrez identifiers or gene symbols as well as probes with small standard deviation across samples were filtered out from the analysis. Further, features mapping to the same Entrez identifier were consolidated by leaving the one with largest value and, finally, probes with standard deviation less than 0.5 across samples were filtered out from the analysis. Differentially expressed genes were identified using the Bioconductor limma package and ranked by calculating the B statistics for each gene. Gene annotation was performed using the Bioconductor mouse4302.db package, while the hypergeometric test of the Bioconductor’s GOstats package was used for identifying overrepresentation of differentially expressed genes in gene ontology terms.
| Sample_platform_id | GPL1261
| Sample_contact_name | Boian,S,Alexandrov
| Sample_contact_email | boian@lanl.gov
| Sample_contact_department | Theoretical Division
| Sample_contact_institute | LANL
| Sample_contact_address | Bikini Atoll Road
| Sample_contact_city | Los Alamos
| Sample_contact_state | NM
| Sample_contact_zip/postal_code | 87545
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008424/suppl/GSM1008424_18_Mouse430_2.CEL.gz
| Sample_series_id | GSE41085
| Sample_series_id | GSE41106
| Sample_data_row_count | 45101
| |
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