Result

Search results for the GEO ID: GSE41095

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1008501

GPL1261

Embryo at E10.5, control, biological rep1

control pregnancy pool C1 E10.5

strain: FVB age: E10.5 tissue: whole embryo disease state: control pregnancy

Sample_geo_accession	GSM1008501
Sample_status	Public on Sep 25 2012
Sample_submission_date	Sep 24 2012
Sample_last_update_date	Sep 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Embryos were dissected at 10.5 days of gestation and stored in Trizol at minus 80 degrees Celsius.
Sample_growth_protocol_ch1	Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with presence of a vaginal plug was counted as 0.5 days of gestation, after which pregnant dams were single-housed.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Five ug of total RNA was reverse transcribed and biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays (Mouse 430A2.0) were hybridized overnight in the Affymetrix hybridization oven at 42 degrees Celsius. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	Images were analyzed using Affymetrix GCOS imaging software. Statistical analyses were performed using GeneSpring7 and CyberT.
Sample_data_processing	MAS 5.0
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008501/suppl/GSM1008501_Pool_C1.CEL.gz
Sample_series_id	GSE41095
Sample_data_row_count	45101

GSM1008502

GPL1261

Embryo at E10.5, control, biological rep2

control pregnancy pool C2 E10.5

strain: FVB age: E10.5 tissue: whole embryo disease state: control pregnancy

Sample_geo_accession	GSM1008502
Sample_status	Public on Sep 25 2012
Sample_submission_date	Sep 24 2012
Sample_last_update_date	Sep 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Embryos were dissected at 10.5 days of gestation and stored in Trizol at minus 80 degrees Celsius.
Sample_growth_protocol_ch1	Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with presence of a vaginal plug was counted as 0.5 days of gestation, after which pregnant dams were single-housed.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Five ug of total RNA was reverse transcribed and biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays (Mouse 430A2.0) were hybridized overnight in the Affymetrix hybridization oven at 42 degrees Celsius. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	Images were analyzed using Affymetrix GCOS imaging software. Statistical analyses were performed using GeneSpring7 and CyberT.
Sample_data_processing	MAS 5.0
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008502/suppl/GSM1008502_Pool_C2.CEL.gz
Sample_series_id	GSE41095
Sample_data_row_count	45101

GSM1008503

GPL1261

Embryo at E10.5, control, biological rep3

control pregnancy pool C3 E10.5

strain: FVB age: E10.5 tissue: whole embryo disease state: control pregnancy

Sample_geo_accession	GSM1008503
Sample_status	Public on Sep 25 2012
Sample_submission_date	Sep 24 2012
Sample_last_update_date	Sep 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Embryos were dissected at 10.5 days of gestation and stored in Trizol at minus 80 degrees Celsius.
Sample_growth_protocol_ch1	Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with presence of a vaginal plug was counted as 0.5 days of gestation, after which pregnant dams were single-housed.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Five ug of total RNA was reverse transcribed and biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays (Mouse 430A2.0) were hybridized overnight in the Affymetrix hybridization oven at 42 degrees Celsius. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	Images were analyzed using Affymetrix GCOS imaging software. Statistical analyses were performed using GeneSpring7 and CyberT.
Sample_data_processing	MAS 5.0
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008503/suppl/GSM1008503_Pool_C3.CEL.gz
Sample_series_id	GSE41095
Sample_data_row_count	45101

GSM1008504

GPL1261

Embryo at E10.5, diabetic, biological rep1

diabetic pregnancy pool D1 E10.5

strain: FVB age: E10.5 tissue: whole embryo disease state: diabetic pregnancy

Sample_geo_accession	GSM1008504
Sample_status	Public on Sep 25 2012
Sample_submission_date	Sep 24 2012
Sample_last_update_date	Sep 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Embryos were dissected at 10.5 days of gestation and stored in Trizol at minus 80 degrees Celsius.
Sample_growth_protocol_ch1	Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with presence of a vaginal plug was counted as 0.5 days of gestation, after which pregnant dams were single-housed.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Five ug of total RNA was reverse transcribed and biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays (Mouse 430A2.0) were hybridized overnight in the Affymetrix hybridization oven at 42 degrees Celsius. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	Images were analyzed using Affymetrix GCOS imaging software. Statistical analyses were performed using GeneSpring7 and CyberT.
Sample_data_processing	MAS 5.0
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008504/suppl/GSM1008504_Pool_D1.CEL.gz
Sample_series_id	GSE41095
Sample_data_row_count	45101

GSM1008505

GPL1261

Embryo at E10.5, diabetic, biological rep2

diabetic pregnancy pool D2 E10.5

strain: FVB age: E10.5 tissue: whole embryo disease state: diabetic pregnancy

Sample_geo_accession	GSM1008505
Sample_status	Public on Sep 25 2012
Sample_submission_date	Sep 24 2012
Sample_last_update_date	Sep 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Embryos were dissected at 10.5 days of gestation and stored in Trizol at minus 80 degrees Celsius.
Sample_growth_protocol_ch1	Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with presence of a vaginal plug was counted as 0.5 days of gestation, after which pregnant dams were single-housed.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Five ug of total RNA was reverse transcribed and biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays (Mouse 430A2.0) were hybridized overnight in the Affymetrix hybridization oven at 42 degrees Celsius. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	Images were analyzed using Affymetrix GCOS imaging software. Statistical analyses were performed using GeneSpring7 and CyberT.
Sample_data_processing	MAS 5.0
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008505/suppl/GSM1008505_Pool_D2.CEL.gz
Sample_series_id	GSE41095
Sample_data_row_count	45101

GSM1008506

GPL1261

Embryo at E10.5, diabetic, biological rep3

diabetic pregnancy pool D3 E10.5

strain: FVB age: E10.5 tissue: whole embryo disease state: diabetic pregnancy

Sample_geo_accession	GSM1008506
Sample_status	Public on Sep 25 2012
Sample_submission_date	Sep 24 2012
Sample_last_update_date	Sep 25 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Embryos were dissected at 10.5 days of gestation and stored in Trizol at minus 80 degrees Celsius.
Sample_growth_protocol_ch1	Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with presence of a vaginal plug was counted as 0.5 days of gestation, after which pregnant dams were single-housed.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Five ug of total RNA was reverse transcribed and biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
Sample_hyb_protocol	GeneChip arrays (Mouse 430A2.0) were hybridized overnight in the Affymetrix hybridization oven at 42 degrees Celsius. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the GeneChip Scanner 3000 7G.
Sample_data_processing	Images were analyzed using Affymetrix GCOS imaging software. Statistical analyses were performed using GeneSpring7 and CyberT.
Sample_data_processing	MAS 5.0
Sample_platform_id	GPL1261
Sample_contact_name	Claudia,,Kappen
Sample_contact_email	claudia.kappen@pbrc.edu
Sample_contact_phone	225-763-2781
Sample_contact_department	Developmental Biology
Sample_contact_institute	Pennington Biomedical Research Center
Sample_contact_address	6400 Perkins Road
Sample_contact_city	Baton Rouge
Sample_contact_state	LA
Sample_contact_zip/postal_code	70808
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1008nnn/GSM1008506/suppl/GSM1008506_Pool_D3.CEL.gz
Sample_series_id	GSE41095
Sample_data_row_count	45101

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