Result

Search results for the GEO ID: GSE41164

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1009613

GPL1261

mU50_wildtype_splenoB

Splenic B-cells from C57BL/6J wildtype mice

strain: C57BL/6J age: 12-15 Wks tissue: Spleen

Gene expression data from three mice

Sample_geo_accession	GSM1009613
Sample_status	Public on Mar 26 2013
Sample_submission_date	Sep 26 2012
Sample_last_update_date	Mar 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were euthanized by cervical dislocation and the spleen dissected out were placed in Hanks’ balanced salt solution (HBSS) at 4C. A dissected spleen was gently ground by two slide-glasses and collected into a test tube with HBSS. The cell pellet of the splenocytes was treated with 4 ml of ACK lysing buffer (Lonza) for 5 min to lyse out the erythrocytes. After several washing of the cells with HBSS, FITC-conjugated anti-CD45R/B220 antibody (BD Biosciences) was added and reacted for 15 min on ice. Non-reacted antibody was washed out with HBSS, and then the cells were reacted with anti-FITC antibody-coupled magnetic beads (Miltenyi Biotec) for 10 min. After rinsing twice, B-cells were separated using a LS+MACS separation column (Myltenyi Biotec).
Sample_growth_protocol_ch1	Mice were kept under a 12-h light-dark cycle and were given standard laboratory fodder and water ad libitum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the sample using RNeasy Micro Kit (QIAGEN) according to the manufacturer's instruction.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA.
Sample_hyb_protocol	cRNA were hybridized for 16 hr at 45C on GeneChip Microarray (Mouse expression 430 2.0, Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were RMA normalized using Expression Console v. 1.1.2800.19935. RMA normalized data were analyzed using GeneSpring software version 7.3.1 (Silicon Genetics Inc.).
Sample_platform_id	GPL1261
Sample_contact_name	Yuuichi,,Soeno
Sample_contact_email	patho-aoba@tky.ndu.ac.jp
Sample_contact_phone	81-3-3261-8921
Sample_contact_fax	81-3-3261-8969
Sample_contact_department	Pathology
Sample_contact_institute	The Nippon Dental University
Sample_contact_address	1-9-20 Fujimi, Chiyoda-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	102-8159
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1009nnn/GSM1009613/suppl/GSM1009613_Mus_mU50_W_SpB.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1009nnn/GSM1009613/suppl/GSM1009613_Mus_mU50_W_SpB_rma.chp.gz
Sample_series_id	GSE41164
Sample_data_row_count	45101

GSM1009614

GPL1261

mU50_null_splenoB

Splenic B-cells from DmU50(HG-b) mice

strain: C57BL/6J age: 12-15 Wks tissue: Spleen

Gene expression data from three mice

Sample_geo_accession	GSM1009614
Sample_status	Public on Mar 26 2013
Sample_submission_date	Sep 26 2012
Sample_last_update_date	Mar 26 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were euthanized by cervical dislocation and the spleen dissected out were placed in Hanks’ balanced salt solution (HBSS) at 4C. A dissected spleen was gently ground by two slide-glasses and collected into a test tube with HBSS. The cell pellet of the splenocytes was treated with 4 ml of ACK lysing buffer (Lonza) for 5 min to lyse out the erythrocytes. After several washing of the cells with HBSS, FITC-conjugated anti-CD45R/B220 antibody (BD Biosciences) was added and reacted for 15 min on ice. Non-reacted antibody was washed out with HBSS, and then the cells were reacted with anti-FITC antibody-coupled magnetic beads (Miltenyi Biotec) for 10 min. After rinsing twice, B-cells were separated using a LS+MACS separation column (Myltenyi Biotec).
Sample_growth_protocol_ch1	Mice were kept under a 12-h light-dark cycle and were given standard laboratory fodder and water ad libitum.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from the sample using RNeasy Micro Kit (QIAGEN) according to the manufacturer's instruction.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA.
Sample_hyb_protocol	cRNA were hybridized for 16 hr at 45C on GeneChip Microarray (Mouse expression 430 2.0, Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Sample_data_processing	The data were RMA normalized using Expression Console v. 1.1.2800.19935. RMA normalized data were analyzed using GeneSpring software version 7.3.1 (Silicon Genetics Inc.).
Sample_platform_id	GPL1261
Sample_contact_name	Yuuichi,,Soeno
Sample_contact_email	patho-aoba@tky.ndu.ac.jp
Sample_contact_phone	81-3-3261-8921
Sample_contact_fax	81-3-3261-8969
Sample_contact_department	Pathology
Sample_contact_institute	The Nippon Dental University
Sample_contact_address	1-9-20 Fujimi, Chiyoda-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	102-8159
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1009nnn/GSM1009614/suppl/GSM1009614_Mus_mU50_N_SpB.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1009nnn/GSM1009614/suppl/GSM1009614_Mus_mU50_N_SpB_rma.chp.gz
Sample_series_id	GSE41164
Sample_data_row_count	45101

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