Search results for the GEO ID: GSE41179 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1010122 | GPL1261 |
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control neural crest cell at E10.5, biological rep1
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control neural crest cells
|
tissue: outflow tract (OFT)
cell type: cardiac neural crest cell
genotype/variation: wildtype
developmental stage: embryo
age: E10.5
|
Gene expression data from cardiac neural crest at E10.5
Wt1 OFT
|
Sample_geo_accession | GSM1010122
| Sample_status | Public on Oct 01 2012
| Sample_submission_date | Sep 27 2012
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The OFTs of NKO mutant and control embryos at E10.5 were digested with collagenase/dispase (1mg/ml)/trypsin (0.1%) for 10 minutes (x3). The cell suspension was filtered through a 40um filter unit, centrifuged and resuspended in up to 0.5ml staining medium, and stained with FDG using FluoReporter LacZ Flow Cytometry kits (Molecular Probes, F-1930). The treated cell suspensions were FACS sorted (BD FACSAria) and stored at 80°C.
| Sample_growth_protocol_ch1 | embryos at E10.5 were dissected and genotyped
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing = program-name = Expression Console; affymetrix-algorithm-name | plier-mm
| Sample_platform_id | GPL1261
| Sample_contact_name | yunfu,,sun
| Sample_contact_email | yfsunjie@gmail.com
| Sample_contact_department | school of medicine
| Sample_contact_institute | tongji university
| Sample_contact_address | 150 jimo road, pudong new district
| Sample_contact_city | shanghai
| Sample_contact_state | shanghai
| Sample_contact_zip/postal_code | 200120
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010122/suppl/GSM1010122_8842.Chen.WT1.as_Mouse430_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010122/suppl/GSM1010122_8842.Chen.WT1.as_Mouse430_2_.plier-mm.chp.gz
| Sample_series_id | GSE41179
| Sample_data_row_count | 45101
| |
|
GSM1010123 | GPL1261 |
|
control neural crest cell at E10.5, biological rep2
|
control neural crest cells
|
tissue: outflow tract (OFT)
cell type: cardiac neural crest cell
genotype/variation: wildtype
developmental stage: embryo
age: E10.5
|
Gene expression data from cardiac neural crest at E10.5
Wt2 OFT
|
Sample_geo_accession | GSM1010123
| Sample_status | Public on Oct 01 2012
| Sample_submission_date | Sep 27 2012
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The OFTs of NKO mutant and control embryos at E10.5 were digested with collagenase/dispase (1mg/ml)/trypsin (0.1%) for 10 minutes (x3). The cell suspension was filtered through a 40um filter unit, centrifuged and resuspended in up to 0.5ml staining medium, and stained with FDG using FluoReporter LacZ Flow Cytometry kits (Molecular Probes, F-1930). The treated cell suspensions were FACS sorted (BD FACSAria) and stored at 80°C.
| Sample_growth_protocol_ch1 | embryos at E10.5 were dissected and genotyped
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing = program-name = Expression Console; affymetrix-algorithm-name | plier-mm
| Sample_platform_id | GPL1261
| Sample_contact_name | yunfu,,sun
| Sample_contact_email | yfsunjie@gmail.com
| Sample_contact_department | school of medicine
| Sample_contact_institute | tongji university
| Sample_contact_address | 150 jimo road, pudong new district
| Sample_contact_city | shanghai
| Sample_contact_state | shanghai
| Sample_contact_zip/postal_code | 200120
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010123/suppl/GSM1010123_8843.Chen.WT2.as_Mouse430_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010123/suppl/GSM1010123_8843.Chen.WT2.as_Mouse430_2_.plier-mm.chp.gz
| Sample_series_id | GSE41179
| Sample_data_row_count | 45101
| |
|
GSM1010124 | GPL1261 |
|
mut neural crest cell at E10.5, biological rep1
|
ILK mutant neural crest cells
|
tissue: outflow tract (OFT)
cell type: cardiac neural crest cell
genotype/variation: ILK deletion
developmental stage: embryo
age: E10.5
|
Gene expression data from cardiac neural crest at E10.5
Mut1 OFT
|
Sample_geo_accession | GSM1010124
| Sample_status | Public on Oct 01 2012
| Sample_submission_date | Sep 27 2012
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The OFTs of NKO mutant and control embryos at E10.5 were digested with collagenase/dispase (1mg/ml)/trypsin (0.1%) for 10 minutes (x3). The cell suspension was filtered through a 40um filter unit, centrifuged and resuspended in up to 0.5ml staining medium, and stained with FDG using FluoReporter LacZ Flow Cytometry kits (Molecular Probes, F-1930). The treated cell suspensions were FACS sorted (BD FACSAria) and stored at 80°C.
| Sample_growth_protocol_ch1 | embryos at E10.5 were dissected and genotyped
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing = program-name = Expression Console; affymetrix-algorithm-name | plier-mm
| Sample_platform_id | GPL1261
| Sample_contact_name | yunfu,,sun
| Sample_contact_email | yfsunjie@gmail.com
| Sample_contact_department | school of medicine
| Sample_contact_institute | tongji university
| Sample_contact_address | 150 jimo road, pudong new district
| Sample_contact_city | shanghai
| Sample_contact_state | shanghai
| Sample_contact_zip/postal_code | 200120
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010124/suppl/GSM1010124_8844.Chen.Mut1.as_Mouse430_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010124/suppl/GSM1010124_8844.Chen.Mut1.as_Mouse430_2_.plier-mm.chp.gz
| Sample_series_id | GSE41179
| Sample_data_row_count | 45101
| |
|
GSM1010125 | GPL1261 |
|
mut neural crest cell at E10.5, biological rep2
|
ILK mutant neural crest cells
|
tissue: outflow tract (OFT)
cell type: cardiac neural crest cell
genotype/variation: ILK deletion
developmental stage: embryo
age: E10.5
|
Gene expression data from cardiac neural crest at E10.5
Mut2 OFT
|
Sample_geo_accession | GSM1010125
| Sample_status | Public on Oct 01 2012
| Sample_submission_date | Sep 27 2012
| Sample_last_update_date | Oct 01 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | The OFTs of NKO mutant and control embryos at E10.5 were digested with collagenase/dispase (1mg/ml)/trypsin (0.1%) for 10 minutes (x3). The cell suspension was filtered through a 40um filter unit, centrifuged and resuspended in up to 0.5ml staining medium, and stained with FDG using FluoReporter LacZ Flow Cytometry kits (Molecular Probes, F-1930). The treated cell suspensions were FACS sorted (BD FACSAria) and stored at 80°C.
| Sample_growth_protocol_ch1 | embryos at E10.5 were dissected and genotyped
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing = program-name = Expression Console; affymetrix-algorithm-name | plier-mm
| Sample_platform_id | GPL1261
| Sample_contact_name | yunfu,,sun
| Sample_contact_email | yfsunjie@gmail.com
| Sample_contact_department | school of medicine
| Sample_contact_institute | tongji university
| Sample_contact_address | 150 jimo road, pudong new district
| Sample_contact_city | shanghai
| Sample_contact_state | shanghai
| Sample_contact_zip/postal_code | 200120
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010125/suppl/GSM1010125_8845.Chen.Mut2.as_Mouse430_2_.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010125/suppl/GSM1010125_8845.Chen.Mut2.as_Mouse430_2_.plier-mm.chp.gz
| Sample_series_id | GSE41179
| Sample_data_row_count | 45101
| |
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