Search results for the GEO ID: GSE41226  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1011094 | GPL570 |
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SKOV3_G9a shRNA1
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SKOV3 cells with G9a shRNA
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cell line: SKOV3
cell type: ovarian cancer
genotype/variation: expressing G9a shRNA
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Gene expression data from SKOV3 cells with G9a depletion
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| Sample_geo_accession | GSM1011094
| | Sample_status | Public on Jul 18 2013
| | Sample_submission_date | Sep 28 2012
| | Sample_last_update_date | Jul 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
| | Sample_growth_protocol_ch1 | Cells were cultured in 5% CO2 incubator at 37℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | kuo-tai,,hua
| | Sample_contact_email | d94447003@gmail.com
| | Sample_contact_institute | National Taiwan University
| | Sample_contact_address | No.2, Xu-Zhou Road
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 10055
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1011nnn/GSM1011094/suppl/GSM1011094_G9a_shRNA67.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1011nnn/GSM1011094/suppl/GSM1011094_G9a_shRNA67.CHP.gz
| | Sample_series_id | GSE41226
| | Sample_data_row_count | 54675
| | |
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GSM1011095 | GPL570 |
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SKOV3_G9a shRNA2
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SKOV3 cells with G9a shRNA
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cell line: SKOV3
cell type: ovarian cancer
genotype/variation: expressing G9a shRNA
|
Gene expression data from SKOV3 cells with G9a depletion
|
| Sample_geo_accession | GSM1011095
| | Sample_status | Public on Jul 18 2013
| | Sample_submission_date | Sep 28 2012
| | Sample_last_update_date | Jul 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
| | Sample_growth_protocol_ch1 | Cells were cultured in 5% CO2 incubator at 37℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | kuo-tai,,hua
| | Sample_contact_email | d94447003@gmail.com
| | Sample_contact_institute | National Taiwan University
| | Sample_contact_address | No.2, Xu-Zhou Road
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 10055
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1011nnn/GSM1011095/suppl/GSM1011095_G9a_shRNA69.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1011nnn/GSM1011095/suppl/GSM1011095_G9a_shRNA69.CHP.gz
| | Sample_series_id | GSE41226
| | Sample_data_row_count | 54675
| | |
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GSM1011096 | GPL570 |
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SKOV3_Luc shRNA
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SKOV3 cells with Luc shRNA
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cell line: SKOV3
cell type: ovarian cancer
genotype/variation: expressing luciferase shRNA
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Gene expression data from SKOV3 cells
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| Sample_geo_accession | GSM1011096
| | Sample_status | Public on Jul 18 2013
| | Sample_submission_date | Sep 28 2012
| | Sample_last_update_date | Jul 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cell were selected with puromycin for two weeks. Stably transduced cells were use for this experiment.
| | Sample_growth_protocol_ch1 | Cells were cultured in 5% CO2 incubator at 37℃.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagene RNeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 PLUS 2.0 . GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | kuo-tai,,hua
| | Sample_contact_email | d94447003@gmail.com
| | Sample_contact_institute | National Taiwan University
| | Sample_contact_address | No.2, Xu-Zhou Road
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 10055
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1011nnn/GSM1011096/suppl/GSM1011096_Luc_shRNA.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1011nnn/GSM1011096/suppl/GSM1011096_Luc_shRNA.CHP.gz
| | Sample_series_id | GSE41226
| | Sample_data_row_count | 54675
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