Result

Search results for the GEO ID: GSE41485

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Title

Source name

Description

Characteristics

GSM1018105

GPL570

ACHN ccRCC cells treated with DMSO

ACHN ccRCC cells treated with DMSO for 24H

cell line: ACHN treatment: DMSO

Sample_geo_accession	GSM1018105
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018105/suppl/GSM1018105_Cop010173HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018106

GPL570

ACHN ccRCC cells treated with A939572

ACHN ccRCC cells treated with A939572 for 24H

cell line: ACHN treatment: A939572

Sample_geo_accession	GSM1018106
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018106/suppl/GSM1018106_Cop010174HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018107

GPL570

CAKI2 ccRCC cells treated with DMSO

CAKI2 ccRCC cells treated with DMSO for 24H

cell line: CAKI2 treatment: DMSO

Sample_geo_accession	GSM1018107
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018107/suppl/GSM1018107_Cop010177HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018108

GPL570

CAKI2 ccRCC cells treated with A939572

CAKI2 ccRCC cells treated with A939572 for 24H

cell line: CAKI2 treatment: A939572

Sample_geo_accession	GSM1018108
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018108/suppl/GSM1018108_Cop010178HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018109

GPL570

A498 ccRCC cells treated with DMSO

A498 ccRCC cells treated with DMSO for 24H

cell line: A498 treatment: DMSO

Sample_geo_accession	GSM1018109
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018109/suppl/GSM1018109_Cop010181HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018110

GPL570

A498 ccRCC cells treated with A939572

A498 ccRCC cells treated with A939572 for 24H

cell line: A498 treatment: A939572

Sample_geo_accession	GSM1018110
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018110/suppl/GSM1018110_Cop010182HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018111

GPL570

CAKI1 ccRCC cells treated with DMSO

CAKI1 ccRCC cells treated with DMSO for 24H

cell line: CAKI1 treatment: DMSO

Sample_geo_accession	GSM1018111
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018111/suppl/GSM1018111_Cop010185HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

GSM1018112

GPL570

CAKI1 ccRCC cells treated with A939572

CAKI1 ccRCC cells treated with A939572 for 24H

cell line: CAKI1 treatment: A939572

Sample_geo_accession	GSM1018112
Sample_status	Public on Oct 11 2012
Sample_submission_date	Oct 10 2012
Sample_last_update_date	Oct 11 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	RCC cells were treated with 75 nM dose of A939572 SCD1 inhibitor for 24 hours (vs. DMSO control)
Sample_growth_protocol_ch1	RCC cell lines A498, ACHN, Caki1, and Caki2 (ATCC) were maintained in 5% FBS and 1% penicillin-streptomycin supplemented DMEM medium
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA from samples was extracted and LiCl precipitation purified using RNAqueous Midi Kit (Ambion) as per manufacturer protocol, and samples were shipped to Advanced Genomic Technology Center Gene Expression Core at the Mayo Clinic in Rochester, MN for further analysis. RNA quality was assessed by Agilent Bioanalyzer (Santa Clara, CA). Reverse transcription to second strand cDNA was generated from 100 ng of good quality total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The RNA products were column-purified (Affymetrix) and then in vitro transcribed to generate biotin-labeled cRNA.
Sample_hyb_protocol	The IVT products were column-purified, fragmented, and hybridized onto Affymetrix U133 Plus 2.0 GeneChips® at 45º C for 16 h.
Sample_scan_protocol	Subsequent to hybridization, the arrays were washed and stained with streptavidin-phycoerythrin, then scanned in an Affymetrix GeneChip® Scanner 3000 (Santa Clara, CA). All control parameters were confirmed to be within normal ranges before normalization and data reduction was initiated.
Sample_data_processing	Raw data was processed by MAS.5 (Affymetrix) and analyzed using GeneSpring GX10
Sample_platform_id	GPL570
Sample_contact_name	Christina,,von Roemeling
Sample_contact_laboratory	Copland Lab
Sample_contact_department	Cancer Research
Sample_contact_institute	The Mayo Clinic, Jacksonville FL
Sample_contact_address	4500 San Pablo Rd
Sample_contact_city	Jacksonville
Sample_contact_state	Florida
Sample_contact_zip/postal_code	32224
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018112/suppl/GSM1018112_Cop010186HG-U133_Plus_2.cel.gz
Sample_series_id	GSE41485
Sample_data_row_count	54675

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