Search results for the GEO ID: GSE41492  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1018194 | GPL1261 |
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Nr1+
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IL10+CD25+CD4+ T-cells from C57Bl/6 mice
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cell type: IL10+CD25+CD4+ T-cells
strain: C57Bl/6
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Gene expression data from IL10+CD25+CD4+ T-cells from C57Bl/6 mice
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| Sample_geo_accession | GSM1018194
| | Sample_status | Public on Aug 10 2013
| | Sample_submission_date | Oct 11 2012
| | Sample_last_update_date | Aug 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cultured CD4+CD25+ T cells were stained for IL-10 and FACS sorted into IL-10+ and IL-10- CD25+ T cells.
| | Sample_growth_protocol_ch1 | CD28 Superagonist D665 was injected i.p. into 6-12 weeks old C57BL/6 mice. After 3 days CD4+CD25+ of lymph nodes were isolated and cultured over night with anti-CD3 (2 µg/ml), anti-CD28 (5 µg/ml) and recombinant human IL-2 (200 U/ml) at 37° and 5% CO2 at 3e6 cells/well in 2 ml complete RPMI medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were lysed in Trizol reagent and complete RNA was isolated according to manufacturers instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| | Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and vsn as normalization method
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Susanne,Elma,Kneitz
| | Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| | Sample_contact_phone | +49-931-31 86526
| | Sample_contact_department | Physiologigcal Chemistry
| | Sample_contact_institute | University of Wuerzburg
| | Sample_contact_address | Biozentrum, Am Hubland
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018194/suppl/GSM1018194_Nr1+.CEL.gz
| | Sample_series_id | GSE41492
| | Sample_data_row_count | 45101
| | |
|
GSM1018195 | GPL1261 |
|
Nr2+
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IL10+CD25+CD4+ T-cells from C57Bl/6 mice
|
cell type: IL10+CD25+CD4+ T-cells
strain: C57Bl/6
|
Gene expression data from IL10+CD25+CD4+ T-cells from C57Bl/6 mice
|
| Sample_geo_accession | GSM1018195
| | Sample_status | Public on Aug 10 2013
| | Sample_submission_date | Oct 11 2012
| | Sample_last_update_date | Aug 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cultured CD4+CD25+ T cells were stained for IL-10 and FACS sorted into IL-10+ and IL-10- CD25+ T cells.
| | Sample_growth_protocol_ch1 | CD28 Superagonist D665 was injected i.p. into 6-12 weeks old C57BL/6 mice. After 3 days CD4+CD25+ of lymph nodes were isolated and cultured over night with anti-CD3 (2 µg/ml), anti-CD28 (5 µg/ml) and recombinant human IL-2 (200 U/ml) at 37° and 5% CO2 at 3e6 cells/well in 2 ml complete RPMI medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were lysed in Trizol reagent and complete RNA was isolated according to manufacturers instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| | Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and vsn as normalization method
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Susanne,Elma,Kneitz
| | Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| | Sample_contact_phone | +49-931-31 86526
| | Sample_contact_department | Physiologigcal Chemistry
| | Sample_contact_institute | University of Wuerzburg
| | Sample_contact_address | Biozentrum, Am Hubland
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018195/suppl/GSM1018195_Nr2+.CEL.gz
| | Sample_series_id | GSE41492
| | Sample_data_row_count | 45101
| | |
|
GSM1018196 | GPL1261 |
|
Nr3-
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IL10-CD25+CD4+ T-cells from C57Bl/6 mice
|
cell type: IL10-CD25+CD4+ T-cells
strain: C57Bl/6
|
Gene expression data from IL10-CD25+CD4+ T-cells from C57Bl/6 mice
|
| Sample_geo_accession | GSM1018196
| | Sample_status | Public on Aug 10 2013
| | Sample_submission_date | Oct 11 2012
| | Sample_last_update_date | Aug 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cultured CD4+CD25+ T cells were stained for IL-10 and FACS sorted into IL-10+ and IL-10- CD25+ T cells.
| | Sample_growth_protocol_ch1 | CD28 Superagonist D665 was injected i.p. into 6-12 weeks old C57BL/6 mice. After 3 days CD4+CD25+ of lymph nodes were isolated and cultured over night with anti-CD3 (2 µg/ml), anti-CD28 (5 µg/ml) and recombinant human IL-2 (200 U/ml) at 37° and 5% CO2 at 3e6 cells/well in 2 ml complete RPMI medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were lysed in Trizol reagent and complete RNA was isolated according to manufacturers instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| | Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and vsn as normalization method
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Susanne,Elma,Kneitz
| | Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| | Sample_contact_phone | +49-931-31 86526
| | Sample_contact_department | Physiologigcal Chemistry
| | Sample_contact_institute | University of Wuerzburg
| | Sample_contact_address | Biozentrum, Am Hubland
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018196/suppl/GSM1018196_Nr3-.CEL.gz
| | Sample_series_id | GSE41492
| | Sample_data_row_count | 45101
| | |
|
GSM1018197 | GPL1261 |
|
Nr4-
|
IL10-CD25+CD4+ T-cells from C57Bl/6 mice
|
cell type: IL10-CD25+CD4+ T-cells
strain: C57Bl/6
|
Gene expression data from IL10-CD25+CD4+ T-cells from C57Bl/6 mice
|
| Sample_geo_accession | GSM1018197
| | Sample_status | Public on Aug 10 2013
| | Sample_submission_date | Oct 11 2012
| | Sample_last_update_date | Aug 10 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cultured CD4+CD25+ T cells were stained for IL-10 and FACS sorted into IL-10+ and IL-10- CD25+ T cells.
| | Sample_growth_protocol_ch1 | CD28 Superagonist D665 was injected i.p. into 6-12 weeks old C57BL/6 mice. After 3 days CD4+CD25+ of lymph nodes were isolated and cultured over night with anti-CD3 (2 µg/ml), anti-CD28 (5 µg/ml) and recombinant human IL-2 (200 U/ml) at 37° and 5% CO2 at 3e6 cells/well in 2 ml complete RPMI medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were lysed in Trizol reagent and complete RNA was isolated according to manufacturers instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
| | Sample_scan_protocol | GeneChips were scanned using the GeneArray Scanner 3000
| | Sample_data_processing | The data were analyzed with Bioconductor packages (under R) and vsn as normalization method
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Susanne,Elma,Kneitz
| | Sample_contact_email | susanne.kneitz@uni-wuerzburg.de
| | Sample_contact_phone | +49-931-31 86526
| | Sample_contact_department | Physiologigcal Chemistry
| | Sample_contact_institute | University of Wuerzburg
| | Sample_contact_address | Biozentrum, Am Hubland
| | Sample_contact_city | Wuerzburg
| | Sample_contact_zip/postal_code | 97074
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1018nnn/GSM1018197/suppl/GSM1018197_Nr4-.CEL.gz
| | Sample_series_id | GSE41492
| | Sample_data_row_count | 45101
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