Search results for the GEO ID: GSE41627  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1020347 | GPL570 |
|
WT_mock_LMW
|
polyribosomes were fractionated by sucrose gradient sedimentation and RNA extracted from pools of light polysomes corresponding to 1-3 ribosomes
|
genotype/variation: WT
cell line: MCF10A
cell origin: breast cancer
|
WT_mock_LMW
|
| Sample_geo_accession | GSM1020347
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020347/suppl/GSM1020347_WT_mock_LMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020348 | GPL570 |
|
WT_mock_HMW
|
polyribosomes were fractionated by sucrose gradient sedimentation and RNA extracted from pools of heavy polysomes corresponding to 4 or greater ribosomes
|
genotype/variation: WT
cell line: MCF10A
cell origin: breast cancer
|
WT_mock_HMW
|
| Sample_geo_accession | GSM1020348
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020348/suppl/GSM1020348_WT_mock_HMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020349 | GPL570 |
|
WT_10Gy_LMW
|
light polyribosomes were prepared as described following irradiation of cells
|
genotype/variation: WT
cell line: MCF10A
cell origin: breast cancer
|
WT_10Gy_LMW
|
| Sample_geo_accession | GSM1020349
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020349/suppl/GSM1020349_WT_10Gy_LMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020350 | GPL570 |
|
WT_10Gy_HMW
|
heavy polyribosomes were prepared as described following irradiation of cells
|
genotype/variation: WT
cell line: MCF10A
cell origin: breast cancer
|
WT_10Gy_HMW
|
| Sample_geo_accession | GSM1020350
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020350/suppl/GSM1020350_WT_10Gy_HMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020351 | GPL570 |
|
4GKD_mock_LMW
|
eIF4G1 was silenced by shRNAs then light polyribosomes prepared and fractionated
|
genotype/variation: eIF4G1 KD
cell line: MCF10A
cell origin: breast cancer
|
4GKD_mock_LMW
|
| Sample_geo_accession | GSM1020351
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020351/suppl/GSM1020351_4GKD_mock_LMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020352 | GPL570 |
|
4GKD_mock_HMW
|
eIF4G1 was silenced by shRNAs then heavy polyribosomes prepared and fractionated
|
genotype/variation: eIF4G1 KD
cell line: MCF10A
cell origin: breast cancer
|
4GKD_mock_HMW
|
| Sample_geo_accession | GSM1020352
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020352/suppl/GSM1020352_4GKD_mock_HMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020353 | GPL570 |
|
4GKD_10Gy_LMW
|
cells were irradiated then light polyribosomes were prepared as described above
|
genotype/variation: eIF4G1 KD
cell line: MCF10A
cell origin: breast cancer
|
4GKD_10Gy_LMW
|
| Sample_geo_accession | GSM1020353
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020353/suppl/GSM1020353_4GKD_10Gy_LMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020354 | GPL570 |
|
4GKD_10Gy_HMW
|
cells were irradiated then heavy polyribosomes were prepared as described above
|
genotype/variation: eIF4G1 KD
cell line: MCF10A
cell origin: breast cancer
|
4GKD_10Gy_HMW
|
| Sample_geo_accession | GSM1020354
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020354/suppl/GSM1020354_4GKD_10Gy_HMW.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020355 | GPL570 |
|
WT_mock_Total
|
total RNA was prepared from cells without polyribosome sorting
|
genotype/variation: WT
cell line: MCF10A
cell origin: breast cancer
|
WT_mock_Total
|
| Sample_geo_accession | GSM1020355
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020355/suppl/GSM1020355_WT_mock_Total.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020356 | GPL570 |
|
WT_10Gy_Total
|
total RNA was prepared from cells without polyribosome sorting after irradiation of cells
|
genotype/variation: WT
cell line: MCF10A
cell origin: breast cancer
|
WT_10Gy_Total
|
| Sample_geo_accession | GSM1020356
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020356/suppl/GSM1020356_WT_10Gy_Total.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020357 | GPL570 |
|
4GKD_mock_Total
|
cells were silenced for eIF4G1 then total RNA was prepared from cells without polyribosome sorting
|
genotype/variation: eIF4G1 KD
cell line: MCF10A
cell origin: breast cancer
|
4GKD_mock_Total
|
| Sample_geo_accession | GSM1020357
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020357/suppl/GSM1020357_4GKD_mock_Total.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
| | |
|
GSM1020358 | GPL570 |
|
4GKD_10Gy_Total
|
cells were silenced for eIF4G1, irradiated then total RNA prepared from cells without polyribosome sorting
|
genotype/variation: eIF4G1 KD
cell line: MCF10A
cell origin: breast cancer
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4GKD_10Gy_Total
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| Sample_geo_accession | GSM1020358
| | Sample_status | Public on Oct 17 2012
| | Sample_submission_date | Oct 16 2012
| | Sample_last_update_date | Oct 17 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were irradiated with a Varian Linac 2300 linear accelerator at room temperature; silencing of target eIF4G1 expression was achieved by doxycycline inducible shRNA
| | Sample_growth_protocol_ch1 | cells were grown cultivated under recommended guidelines by the ATCC
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extracted using 0.5% NP40
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeled target cRNA population was generated with the Affymetrix 3’-IVT Express kit
| | Sample_hyb_protocol | Labeled and fragmented cRNA hybridized to HG_U133 Plus 2.0 Affymetrix GeneChips using standard techniques recommended by the manufacturer.
| | Sample_scan_protocol | Affymetrix GeneArray scanner 7G
| | Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring GX11 program. No further adjustments were made to the data in the table.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jiri,,Zavadil
| | Sample_contact_email | jiri.zavadil@med.nyu.edu
| | Sample_contact_phone | 212-263-8048
| | Sample_contact_department | Pathology
| | Sample_contact_institute | NYU SoM
| | Sample_contact_address |
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10016
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1020nnn/GSM1020358/suppl/GSM1020358_4GKD_10Gy_Total.CEL.gz
| | Sample_series_id | GSE41627
| | Sample_data_row_count | 54675
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