Search results for the GEO ID: GSE41704  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1022938 | GPL339 |
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Total epidermis cells, rep1
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FACS sorted living total GFP expressing cells from postnatal d49 K14-GFP-actin mice.
|
genotype/variation: K14-GFP-actin expressing
tissue type: back skin
cell type: basal bulge stem cells
cell population: total GFP expressing
|
total RNA
|
| Sample_geo_accession | GSM1022938
| | Sample_status | Public on Oct 19 2012
| | Sample_submission_date | Oct 19 2012
| | Sample_last_update_date | Oct 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Absolutely RNA Kit (Stratagene)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | mRNA were assessed by RNA 6000 Pico Assay (Agilent) and quantified spectrophotometrically. Primer olig0-dT-T7 (Genset) was used to reverse transcribe (SuperScript cDNA Synthesis Kit, Invitrogen) and then amplify (MessageAmp aRNA Kit, Ambion) 200 ng of RNA. Random priming and biotinylated nucleotides were used to obtain cRNA for microarray.
| | Sample_hyb_protocol | 10 g labeled cRNA was hybridized for 16 hr at 45 C to mouse genome array MOE430a (Affymetrix).
| | Sample_scan_protocol | Processed chips were then read by an argon-ion laser confocal scanner (Genomics Core Facility, MSKCC). The entire procedure was repeated in duplicate for each sample to produce two independent datasets per mRNA sample.
| | Sample_data_processing | Raw microarray images were quantified using Gene Chip Operating Software (GCOS, Affymetrix). Analysis was performed with the R statistical environment (version 2.15.1) and the Bioconductor Affy package (version 1.36) (Irizarry et al, 2004). Normalization was run using affy package standard parameters (quantile normalization and RMA background correction).
| | Sample_platform_id | GPL339
| | Sample_contact_name | KHALIL,,KASS YOUSSEF
| | Sample_contact_email | kellokello@gmail.com
| | Sample_contact_phone | 0032494122112
| | Sample_contact_fax | 00325554655
| | Sample_contact_institute | ULB-IRIBHM
| | Sample_contact_address | 808, Route de Lennick
| | Sample_contact_city | Brussels
| | Sample_contact_zip/postal_code | 1070
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022938/suppl/GSM1022938_WL_MOE_430A_all_gfp_telo_wt1.CEL.gz
| | Sample_series_id | GSE41704
| | Sample_data_row_count | 22690
| | |
|
GSM1022939 | GPL339 |
|
Total epidermis cells, rep2
|
FACS sorted living total GFP expressing cells from postnatal d49 K14-GFP-actin mice.
|
genotype/variation: K14-GFP-actin expressing
tissue type: back skin
cell type: basal bulge stem cells
cell population: total GFP expressing
|
total RNA
|
| Sample_geo_accession | GSM1022939
| | Sample_status | Public on Oct 19 2012
| | Sample_submission_date | Oct 19 2012
| | Sample_last_update_date | Oct 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Absolutely RNA Kit (Stratagene)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | mRNA were assessed by RNA 6000 Pico Assay (Agilent) and quantified spectrophotometrically. Primer olig0-dT-T7 (Genset) was used to reverse transcribe (SuperScript cDNA Synthesis Kit, Invitrogen) and then amplify (MessageAmp aRNA Kit, Ambion) 200 ng of RNA. Random priming and biotinylated nucleotides were used to obtain cRNA for microarray.
| | Sample_hyb_protocol | 10 g labeled cRNA was hybridized for 16 hr at 45 C to mouse genome array MOE430a (Affymetrix).
| | Sample_scan_protocol | Processed chips were then read by an argon-ion laser confocal scanner (Genomics Core Facility, MSKCC). The entire procedure was repeated in duplicate for each sample to produce two independent datasets per mRNA sample.
| | Sample_data_processing | Raw microarray images were quantified using Gene Chip Operating Software (GCOS, Affymetrix). Analysis was performed with the R statistical environment (version 2.15.1) and the Bioconductor Affy package (version 1.36) (Irizarry et al, 2004). Normalization was run using affy package standard parameters (quantile normalization and RMA background correction).
| | Sample_platform_id | GPL339
| | Sample_contact_name | KHALIL,,KASS YOUSSEF
| | Sample_contact_email | kellokello@gmail.com
| | Sample_contact_phone | 0032494122112
| | Sample_contact_fax | 00325554655
| | Sample_contact_institute | ULB-IRIBHM
| | Sample_contact_address | 808, Route de Lennick
| | Sample_contact_city | Brussels
| | Sample_contact_zip/postal_code | 1070
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022939/suppl/GSM1022939_WL_MOE_430A_all_gfp_telo_wt2.CEL.gz
| | Sample_series_id | GSE41704
| | Sample_data_row_count | 22690
| | |
|
GSM1022940 | GPL339 |
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Bulge basal cells, rep1
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FACS sorted living GFP α6high CD34high expressing cells from postnatal d49 K14-GFP-actin mice.
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genotype/variation: K14-GFP-actin expressing
tissue type: back skin
cell type: basal bulge stem cells
cell population: GFP α6high CD34high expressing
|
total RNA
|
| Sample_geo_accession | GSM1022940
| | Sample_status | Public on Oct 19 2012
| | Sample_submission_date | Oct 19 2012
| | Sample_last_update_date | Oct 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Absolutely RNA Kit (Stratagene)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | mRNA were assessed by RNA 6000 Pico Assay (Agilent) and quantified spectrophotometrically. Primer olig0-dT-T7 (Genset) was used to reverse transcribe (SuperScript cDNA Synthesis Kit, Invitrogen) and then amplify (MessageAmp aRNA Kit, Ambion) 200 ng of RNA. Random priming and biotinylated nucleotides were used to obtain cRNA for microarray.
| | Sample_hyb_protocol | 10 g labeled cRNA was hybridized for 16 hr at 45 C to mouse genome array MOE430a (Affymetrix).
| | Sample_scan_protocol | Processed chips were then read by an argon-ion laser confocal scanner (Genomics Core Facility, MSKCC). The entire procedure was repeated in duplicate for each sample to produce two independent datasets per mRNA sample.
| | Sample_data_processing | Raw microarray images were quantified using Gene Chip Operating Software (GCOS, Affymetrix). Analysis was performed with the R statistical environment (version 2.15.1) and the Bioconductor Affy package (version 1.36) (Irizarry et al, 2004). Normalization was run using affy package standard parameters (quantile normalization and RMA background correction).
| | Sample_platform_id | GPL339
| | Sample_contact_name | KHALIL,,KASS YOUSSEF
| | Sample_contact_email | kellokello@gmail.com
| | Sample_contact_phone | 0032494122112
| | Sample_contact_fax | 00325554655
| | Sample_contact_institute | ULB-IRIBHM
| | Sample_contact_address | 808, Route de Lennick
| | Sample_contact_city | Brussels
| | Sample_contact_zip/postal_code | 1070
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022940/suppl/GSM1022940_WL_MOE_430A_a6h_cd34_telo_wt1.CEL.gz
| | Sample_series_id | GSE41704
| | Sample_data_row_count | 22690
| | |
|
GSM1022941 | GPL339 |
|
Bulge basal cells, rep2
|
FACS sorted living GFP α6high CD34high expressing cells from postnatal d49 K14-GFP-actin mice.
|
genotype/variation: K14-GFP-actin expressing
tissue type: back skin
cell type: basal bulge stem cells
cell population: GFP α6high CD34high expressing
|
total RNA
|
| Sample_geo_accession | GSM1022941
| | Sample_status | Public on Oct 19 2012
| | Sample_submission_date | Oct 19 2012
| | Sample_last_update_date | Oct 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Absolutely RNA Kit (Stratagene)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | mRNA were assessed by RNA 6000 Pico Assay (Agilent) and quantified spectrophotometrically. Primer olig0-dT-T7 (Genset) was used to reverse transcribe (SuperScript cDNA Synthesis Kit, Invitrogen) and then amplify (MessageAmp aRNA Kit, Ambion) 200 ng of RNA. Random priming and biotinylated nucleotides were used to obtain cRNA for microarray.
| | Sample_hyb_protocol | 10 g labeled cRNA was hybridized for 16 hr at 45 C to mouse genome array MOE430a (Affymetrix).
| | Sample_scan_protocol | Processed chips were then read by an argon-ion laser confocal scanner (Genomics Core Facility, MSKCC). The entire procedure was repeated in duplicate for each sample to produce two independent datasets per mRNA sample.
| | Sample_data_processing | Raw microarray images were quantified using Gene Chip Operating Software (GCOS, Affymetrix). Analysis was performed with the R statistical environment (version 2.15.1) and the Bioconductor Affy package (version 1.36) (Irizarry et al, 2004). Normalization was run using affy package standard parameters (quantile normalization and RMA background correction).
| | Sample_platform_id | GPL339
| | Sample_contact_name | KHALIL,,KASS YOUSSEF
| | Sample_contact_email | kellokello@gmail.com
| | Sample_contact_phone | 0032494122112
| | Sample_contact_fax | 00325554655
| | Sample_contact_institute | ULB-IRIBHM
| | Sample_contact_address | 808, Route de Lennick
| | Sample_contact_city | Brussels
| | Sample_contact_zip/postal_code | 1070
| | Sample_contact_country | Belgium
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022941/suppl/GSM1022941_WL_MOE_430A_a6h_cd34_telo_wt2.CEL.gz
| | Sample_series_id | GSE41704
| | Sample_data_row_count | 22690
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