Result

Search results for the GEO ID: GSE41706

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Title

Source name

Description

Characteristics

GSM1022960

GPL1261

Adult GRK2+/- heart 1

Adult_GRK2+/-_heart

strain background: B6.129-Adrbk1tm1Mca/J backcrossed more than 20 generations onto C57BL/6J genotype/variation: GRK2 heterozygous (GRK2+/-) age: adult_9 months old tissue: heart

GRK2+/- 1 273.CEL

Sample_geo_accession	GSM1022960
Sample_status	Public on Oct 20 2012
Sample_submission_date	Oct 19 2012
Sample_last_update_date	Oct 24 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
Sample_growth_protocol_ch1	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip® Scanner 3000 7G System.
Sample_data_processing	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Lucas,,Elisa
Sample_contact_email	elucas@cbm.uam.es
Sample_contact_department	Molecular Biology
Sample_contact_institute	Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
Sample_contact_address	Nicolas Cabrera 1
Sample_contact_city	Madrid
Sample_contact_state	Madrid
Sample_contact_zip/postal_code	28049
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022960/suppl/GSM1022960_273.CEL.gz
Sample_relation	Reanalyzed by: GSM1024595
Sample_series_id	GSE41706
Sample_series_id	GSE41810
Sample_data_row_count	45101

GSM1022961

GPL1261

Adult GRK2+/- heart 2

Adult_GRK2+/-_heart

strain background: B6.129-Adrbk1tm1Mca/J backcrossed more than 20 generations onto C57BL/6J genotype/variation: GRK2 heterozygous (GRK2+/-) age: adult_9 months old tissue: heart

GRK2+/- 2 280.CEL

Sample_geo_accession	GSM1022961
Sample_status	Public on Oct 20 2012
Sample_submission_date	Oct 19 2012
Sample_last_update_date	Oct 24 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
Sample_growth_protocol_ch1	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip® Scanner 3000 7G System.
Sample_data_processing	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Lucas,,Elisa
Sample_contact_email	elucas@cbm.uam.es
Sample_contact_department	Molecular Biology
Sample_contact_institute	Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
Sample_contact_address	Nicolas Cabrera 1
Sample_contact_city	Madrid
Sample_contact_state	Madrid
Sample_contact_zip/postal_code	28049
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022961/suppl/GSM1022961_280.CEL.gz
Sample_relation	Reanalyzed by: GSM1024596
Sample_series_id	GSE41706
Sample_series_id	GSE41810
Sample_data_row_count	45101

GSM1022962

GPL1261

Adult GRK2+/- heart 3

Adult_GRK2+/-_heart

strain background: B6.129-Adrbk1tm1Mca/J backcrossed more than 20 generations onto C57BL/6J genotype/variation: GRK2 heterozygous (GRK2+/-) age: adult_9 months old tissue: heart

GRK2+/- 3 289.CEL

Sample_geo_accession	GSM1022962
Sample_status	Public on Oct 20 2012
Sample_submission_date	Oct 19 2012
Sample_last_update_date	Oct 24 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
Sample_growth_protocol_ch1	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip® Scanner 3000 7G System.
Sample_data_processing	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Lucas,,Elisa
Sample_contact_email	elucas@cbm.uam.es
Sample_contact_department	Molecular Biology
Sample_contact_institute	Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
Sample_contact_address	Nicolas Cabrera 1
Sample_contact_city	Madrid
Sample_contact_state	Madrid
Sample_contact_zip/postal_code	28049
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022962/suppl/GSM1022962_289.CEL.gz
Sample_relation	Reanalyzed by: GSM1024597
Sample_series_id	GSE41706
Sample_series_id	GSE41810
Sample_data_row_count	45101

GSM1022963

GPL1261

Adult WT heart 1

Adult_WT_heart

strain background: C57BL/6J genotype/variation: wild type age: adult_9 months old tissue: heart

WT 1 WT_276.CEL

Sample_geo_accession	GSM1022963
Sample_status	Public on Oct 20 2012
Sample_submission_date	Oct 19 2012
Sample_last_update_date	Oct 24 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
Sample_growth_protocol_ch1	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip® Scanner 3000 7G System.
Sample_data_processing	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Lucas,,Elisa
Sample_contact_email	elucas@cbm.uam.es
Sample_contact_department	Molecular Biology
Sample_contact_institute	Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
Sample_contact_address	Nicolas Cabrera 1
Sample_contact_city	Madrid
Sample_contact_state	Madrid
Sample_contact_zip/postal_code	28049
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022963/suppl/GSM1022963_WT_276.CEL.gz
Sample_relation	Reanalyzed by: GSM1024589
Sample_series_id	GSE41706
Sample_series_id	GSE41810
Sample_data_row_count	45101

GSM1022964

GPL1261

Adult WT heart 2

Adult_WT_heart

strain background: C57BL/6J genotype/variation: wild type age: adult_9 months old tissue: heart

WT 2 WT_282.CEL

Sample_geo_accession	GSM1022964
Sample_status	Public on Oct 20 2012
Sample_submission_date	Oct 19 2012
Sample_last_update_date	Oct 24 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
Sample_growth_protocol_ch1	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip® Scanner 3000 7G System.
Sample_data_processing	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Lucas,,Elisa
Sample_contact_email	elucas@cbm.uam.es
Sample_contact_department	Molecular Biology
Sample_contact_institute	Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
Sample_contact_address	Nicolas Cabrera 1
Sample_contact_city	Madrid
Sample_contact_state	Madrid
Sample_contact_zip/postal_code	28049
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022964/suppl/GSM1022964_WT_282.CEL.gz
Sample_relation	Reanalyzed by: GSM1024590
Sample_series_id	GSE41706
Sample_series_id	GSE41810
Sample_data_row_count	45101

GSM1022965

GPL1261

Adult WT heart 3

Adult_WT_heart

strain background: C57BL/6J genotype/variation: wild type age: adult_9 months old tissue: heart

WT 3 WT_303.CEL

Sample_geo_accession	GSM1022965
Sample_status	Public on Oct 20 2012
Sample_submission_date	Oct 19 2012
Sample_last_update_date	Oct 24 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
Sample_growth_protocol_ch1	The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using GeneChip® Scanner 3000 7G System.
Sample_data_processing	Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
Sample_platform_id	GPL1261
Sample_contact_name	Lucas,,Elisa
Sample_contact_email	elucas@cbm.uam.es
Sample_contact_department	Molecular Biology
Sample_contact_institute	Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
Sample_contact_address	Nicolas Cabrera 1
Sample_contact_city	Madrid
Sample_contact_state	Madrid
Sample_contact_zip/postal_code	28049
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1022nnn/GSM1022965/suppl/GSM1022965_WT_303.CEL.gz
Sample_relation	Reanalyzed by: GSM1024591
Sample_series_id	GSE41706
Sample_series_id	GSE41810
Sample_data_row_count	45101

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