Search results for the GEO ID: GSE41737  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1023406 | GPL570 |
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pre-miR-27a
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pre-miR-27a
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cell line: Huh-7.5 cells
treatment: pre-miR-27a
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Gene expression data from Huh-7.5 cells transfected with pre-miR-27a
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| Sample_geo_accession | GSM1023406
| | Sample_status | Public on Jun 05 2013
| | Sample_submission_date | Oct 22 2012
| | Sample_last_update_date | Jun 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit (QIAGEN) for total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Aliquots of total RNA (50 ng) isolated from cells were subjected to amplification using the WT-Ovation Pico RNA Amplification System (NuGen, San Carlos, CA, USA) according to the manufacturer’s instructions. Approximately 10 ug of cDNA was amplified from 50 ng of total RNA, and 5 ug of cDNA was used for fragmentation and biotin labeling using the FL-Ovation cDNA Biotin Module V2 (NuGen) according to the manufacturer’s instructions.
| | Sample_hyb_protocol | Biotin-labeled cDNA was suspended in 220 ul of hybridization cocktail (NuGen) and 200 ul was used for hybridization to the Affymetrix Human 133 Plus 2.0 microarray chip containing 54,675 probes.GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After stringent washing, the microarray chips were stained with streptavidin-phycoerythrin and probe hybridization was determined using a GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masao,,Honda
| | Sample_contact_email | mhonda@m-kanazawa.jp
| | Sample_contact_phone | 0762652243
| | Sample_contact_fax | 0762344250
| | Sample_contact_department | Gastroenterology
| | Sample_contact_institute | Kanazawa University
| | Sample_contact_address | Takara-Machi 13-1
| | Sample_contact_city | Kanazawa
| | Sample_contact_zip/postal_code | 920-8641
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1023nnn/GSM1023406/suppl/GSM1023406_MasaoHonda_pre_miR_27a.CEL.gz
| | Sample_series_id | GSE41737
| | Sample_data_row_count | 54675
| | |
|
GSM1023407 | GPL570 |
|
pre-miR-control
|
pre-miR-control
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cell line: Huh-7.5 cells
treatment: pre-miR-control
|
Gene expression data from Huh-7.5 cells transfected with pre-miR-control
|
| Sample_geo_accession | GSM1023407
| | Sample_status | Public on Jun 05 2013
| | Sample_submission_date | Oct 22 2012
| | Sample_last_update_date | Jun 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit (QIAGEN) for total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Aliquots of total RNA (50 ng) isolated from cells were subjected to amplification using the WT-Ovation Pico RNA Amplification System (NuGen, San Carlos, CA, USA) according to the manufacturer’s instructions. Approximately 10 ug of cDNA was amplified from 50 ng of total RNA, and 5 ug of cDNA was used for fragmentation and biotin labeling using the FL-Ovation cDNA Biotin Module V2 (NuGen) according to the manufacturer’s instructions.
| | Sample_hyb_protocol | Biotin-labeled cDNA was suspended in 220 ul of hybridization cocktail (NuGen) and 200 ul was used for hybridization to the Affymetrix Human 133 Plus 2.0 microarray chip containing 54,675 probes.GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After stringent washing, the microarray chips were stained with streptavidin-phycoerythrin and probe hybridization was determined using a GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masao,,Honda
| | Sample_contact_email | mhonda@m-kanazawa.jp
| | Sample_contact_phone | 0762652243
| | Sample_contact_fax | 0762344250
| | Sample_contact_department | Gastroenterology
| | Sample_contact_institute | Kanazawa University
| | Sample_contact_address | Takara-Machi 13-1
| | Sample_contact_city | Kanazawa
| | Sample_contact_zip/postal_code | 920-8641
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1023nnn/GSM1023407/suppl/GSM1023407_MasaoHonda_pre_miR_control.CEL.gz
| | Sample_series_id | GSE41737
| | Sample_data_row_count | 54675
| | |
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GSM1023408 | GPL570 |
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anti-miR-27a
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anti-miR-27a
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cell line: Huh-7.5 cells
treatment: anti-miR-27a
|
Gene expression data from Huh-7.5 cells transfected with anti-miR-27a
|
| Sample_geo_accession | GSM1023408
| | Sample_status | Public on Jun 05 2013
| | Sample_submission_date | Oct 22 2012
| | Sample_last_update_date | Jun 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit (QIAGEN) for total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Aliquots of total RNA (50 ng) isolated from cells were subjected to amplification using the WT-Ovation Pico RNA Amplification System (NuGen, San Carlos, CA, USA) according to the manufacturer’s instructions. Approximately 10 ug of cDNA was amplified from 50 ng of total RNA, and 5 ug of cDNA was used for fragmentation and biotin labeling using the FL-Ovation cDNA Biotin Module V2 (NuGen) according to the manufacturer’s instructions.
| | Sample_hyb_protocol | Biotin-labeled cDNA was suspended in 220 ul of hybridization cocktail (NuGen) and 200 ul was used for hybridization to the Affymetrix Human 133 Plus 2.0 microarray chip containing 54,675 probes.GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After stringent washing, the microarray chips were stained with streptavidin-phycoerythrin and probe hybridization was determined using a GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masao,,Honda
| | Sample_contact_email | mhonda@m-kanazawa.jp
| | Sample_contact_phone | 0762652243
| | Sample_contact_fax | 0762344250
| | Sample_contact_department | Gastroenterology
| | Sample_contact_institute | Kanazawa University
| | Sample_contact_address | Takara-Machi 13-1
| | Sample_contact_city | Kanazawa
| | Sample_contact_zip/postal_code | 920-8641
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1023nnn/GSM1023408/suppl/GSM1023408_MasaoHonda_anti_miR_27a.CEL.gz
| | Sample_series_id | GSE41737
| | Sample_data_row_count | 54675
| | |
|
GSM1023409 | GPL570 |
|
anti-miR-control
|
anti-miR-control
|
cell line: Huh-7.5 cells
treatment: anti-miR-control
|
Gene expression data from Huh-7.5 cells transfected with anti-miR-control
|
| Sample_geo_accession | GSM1023409
| | Sample_status | Public on Jun 05 2013
| | Sample_submission_date | Oct 22 2012
| | Sample_last_update_date | Jun 05 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNeasy Mini Kit (QIAGEN) for total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Aliquots of total RNA (50 ng) isolated from cells were subjected to amplification using the WT-Ovation Pico RNA Amplification System (NuGen, San Carlos, CA, USA) according to the manufacturer’s instructions. Approximately 10 ug of cDNA was amplified from 50 ng of total RNA, and 5 ug of cDNA was used for fragmentation and biotin labeling using the FL-Ovation cDNA Biotin Module V2 (NuGen) according to the manufacturer’s instructions.
| | Sample_hyb_protocol | Biotin-labeled cDNA was suspended in 220 ul of hybridization cocktail (NuGen) and 200 ul was used for hybridization to the Affymetrix Human 133 Plus 2.0 microarray chip containing 54,675 probes.GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | After stringent washing, the microarray chips were stained with streptavidin-phycoerythrin and probe hybridization was determined using a GeneChip Scanner 3000 (Affymetrix).
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masao,,Honda
| | Sample_contact_email | mhonda@m-kanazawa.jp
| | Sample_contact_phone | 0762652243
| | Sample_contact_fax | 0762344250
| | Sample_contact_department | Gastroenterology
| | Sample_contact_institute | Kanazawa University
| | Sample_contact_address | Takara-Machi 13-1
| | Sample_contact_city | Kanazawa
| | Sample_contact_zip/postal_code | 920-8641
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1023nnn/GSM1023409/suppl/GSM1023409_MasaoHonda_anti_miR_control.CEL.gz
| | Sample_series_id | GSE41737
| | Sample_data_row_count | 54675
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