Search results for the GEO ID: GSE41807  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1024589 | GPL1261 |
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Adult WT heart 1 [re-analysis]
|
adult heart of 9 months of age
|
genotype: C57BL/6J
tissue: Heart
age: 9 months old; adult
|
9 mo WT 1
WT_276.CEL
|
| Sample_geo_accession | GSM1024589
| | Sample_status | Public on Oct 25 2012
| | Sample_submission_date | Oct 24 2012
| | Sample_last_update_date | Oct 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
| | Sample_growth_protocol_ch1 | The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G System
| | Sample_data_processing | Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lucas,,Elisa
| | Sample_contact_email | elucas@cbm.uam.es
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
| | Sample_contact_address | Nicolas Cabrera 1
| | Sample_contact_city | Madrid
| | Sample_contact_state | Madrid
| | Sample_contact_zip/postal_code | 28049
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1024nnn/GSM1024589/suppl/GSM1024589_WT_276.CEL.gz
| | Sample_relation | Reanalysis of: GSM1022963
| | Sample_series_id | GSE41807
| | Sample_series_id | GSE41810
| | Sample_data_row_count | 45101
| | |
|
GSM1024590 | GPL1261 |
|
Adult WT heart 2 [re-analysis]
|
adult heart of 9 months of age
|
genotype: C57BL/6J
tissue: Heart
age: 9 months old; adult
|
9 mo WT 2
WT_282.CEL
|
| Sample_geo_accession | GSM1024590
| | Sample_status | Public on Oct 25 2012
| | Sample_submission_date | Oct 24 2012
| | Sample_last_update_date | Oct 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
| | Sample_growth_protocol_ch1 | The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G System
| | Sample_data_processing | Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lucas,,Elisa
| | Sample_contact_email | elucas@cbm.uam.es
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
| | Sample_contact_address | Nicolas Cabrera 1
| | Sample_contact_city | Madrid
| | Sample_contact_state | Madrid
| | Sample_contact_zip/postal_code | 28049
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1024nnn/GSM1024590/suppl/GSM1024590_WT_282.CEL.gz
| | Sample_relation | Reanalysis of: GSM1022964
| | Sample_series_id | GSE41807
| | Sample_series_id | GSE41810
| | Sample_data_row_count | 45101
| | |
|
GSM1024591 | GPL1261 |
|
Adult WT heart 3 [re-analysis]
|
adult heart of 9 months of age
|
genotype: C57BL/6J
tissue: Heart
age: 9 months old; adult
|
9 mo WT 3
WT_303.CEL
|
| Sample_geo_accession | GSM1024591
| | Sample_status | Public on Oct 25 2012
| | Sample_submission_date | Oct 24 2012
| | Sample_last_update_date | Oct 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
| | Sample_growth_protocol_ch1 | The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G System
| | Sample_data_processing | Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lucas,,Elisa
| | Sample_contact_email | elucas@cbm.uam.es
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
| | Sample_contact_address | Nicolas Cabrera 1
| | Sample_contact_city | Madrid
| | Sample_contact_state | Madrid
| | Sample_contact_zip/postal_code | 28049
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1024nnn/GSM1024591/suppl/GSM1024591_WT_303.CEL.gz
| | Sample_relation | Reanalysis of: GSM1022965
| | Sample_series_id | GSE41807
| | Sample_series_id | GSE41810
| | Sample_data_row_count | 45101
| | |
|
GSM1024592 | GPL1261 |
|
Young WT heart 1
|
young heart of 4 months of age
|
genotype: C57BL/6J
tissue: Heart
age: 4 months old; young
|
4 mo WT 1
WT_24.CEL
|
| Sample_geo_accession | GSM1024592
| | Sample_status | Public on Oct 25 2012
| | Sample_submission_date | Oct 24 2012
| | Sample_last_update_date | Oct 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
| | Sample_growth_protocol_ch1 | The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G System
| | Sample_data_processing | Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lucas,,Elisa
| | Sample_contact_email | elucas@cbm.uam.es
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
| | Sample_contact_address | Nicolas Cabrera 1
| | Sample_contact_city | Madrid
| | Sample_contact_state | Madrid
| | Sample_contact_zip/postal_code | 28049
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1024nnn/GSM1024592/suppl/GSM1024592_WT_24.CEL.gz
| | Sample_series_id | GSE41807
| | Sample_series_id | GSE41809
| | Sample_series_id | GSE41810
| | Sample_data_row_count | 45101
| | |
|
GSM1024593 | GPL1261 |
|
Young WT heart 2
|
young heart of 4 months of age
|
genotype: C57BL/6J
tissue: Heart
age: 4 months old; young
|
4 mo WT 2
WT_332.CEL
|
| Sample_geo_accession | GSM1024593
| | Sample_status | Public on Oct 25 2012
| | Sample_submission_date | Oct 24 2012
| | Sample_last_update_date | Oct 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
| | Sample_growth_protocol_ch1 | The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G System
| | Sample_data_processing | Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lucas,,Elisa
| | Sample_contact_email | elucas@cbm.uam.es
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
| | Sample_contact_address | Nicolas Cabrera 1
| | Sample_contact_city | Madrid
| | Sample_contact_state | Madrid
| | Sample_contact_zip/postal_code | 28049
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1024nnn/GSM1024593/suppl/GSM1024593_WT_332.CEL.gz
| | Sample_series_id | GSE41807
| | Sample_series_id | GSE41809
| | Sample_series_id | GSE41810
| | Sample_data_row_count | 45101
| | |
|
GSM1024594 | GPL1261 |
|
Young WT heart 3
|
young heart of 4 months of age
|
genotype: C57BL/6J
tissue: Heart
age: 4 months old; young
|
4 mo WT 3
WT_335.CEL
|
| Sample_geo_accession | GSM1024594
| | Sample_status | Public on Oct 25 2012
| | Sample_submission_date | Oct 24 2012
| | Sample_last_update_date | Oct 25 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were anesthetized by inhaling isoflurane and hearts were collected after heart arrest in diastole using cold intracardiac KCl injection. Hearts were rinsed with cold PBS and the third upper part was excised and treated with RNA later (QIAGEN) for 16 hours at 4ºC. Then samples were stored at -70ºC untill RNA extraction.
| | Sample_growth_protocol_ch1 | The animals were bred and housed on a 12-hour light/dark cycle with free access to food and water.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | frozen heart tissue was lysed using metal beads (2 min, 30 Hz) in a Tissue Lyser (QUIAGEN), and total mRNA was extracted using Fibrous Tissue RNeasy Mini Kit (QIAGEN).Sampled with an adequate RNA quality (Bioanalyzer, Agilent 2100) were selected for analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using GeneChip® Scanner 3000 7G System
| | Sample_data_processing | Analysis was performed using the affylmaGUI R package (Wettenhall et al., 2006). Robust Multi-array Analysis (RMA) algorithm was used for background correction, normalization and expression levels summarization (Irizarry et al., 2003). Next, differential expression analysis was performed with the Bayes t-statistics from the linear models for Microarray data (limma), included in the affylmGUI package. P-values were corrected for multiple-testing using the Benjamini-Hochberg’s method (False Discovery Rate) (Benjamini and Hochberg, 1995; Reiner et al., 2003).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Lucas,,Elisa
| | Sample_contact_email | elucas@cbm.uam.es
| | Sample_contact_department | Molecular Biology
| | Sample_contact_institute | Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)
| | Sample_contact_address | Nicolas Cabrera 1
| | Sample_contact_city | Madrid
| | Sample_contact_state | Madrid
| | Sample_contact_zip/postal_code | 28049
| | Sample_contact_country | Spain
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1024nnn/GSM1024594/suppl/GSM1024594_WT_335.CEL.gz
| | Sample_series_id | GSE41807
| | Sample_series_id | GSE41809
| | Sample_series_id | GSE41810
| | Sample_data_row_count | 45101
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