Search results for the GEO ID: GSE41827 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1025049 | GPL570 |
|
HeLa control, biological rep1
|
Untreated HeLa cells
|
cell line: cervical cancer (HeLa) cells
agent: control
|
Gene expression data from untreated Hela cells.
Cas_Control-1
|
Sample_geo_accession | GSM1025049
| Sample_status | Public on Oct 31 2012
| Sample_submission_date | Oct 24 2012
| Sample_last_update_date | Oct 31 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
| Sample_growth_protocol_ch1 | HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
| Sample_hyb_protocol | Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
| Sample_scan_protocol | Scanning procedure was carried out as described by Affymetrix, Santa Clara.
| Sample_data_processing | Data pre-processing was performed by using the affy library in BioConductor running under [R].
| Sample_platform_id | GPL570
| Sample_contact_name | Karol,,Baca Lopez
| Sample_contact_email | kbacal@uaemex.mx
| Sample_contact_institute | National Institute of Genomic Medicine
| Sample_contact_address | Periferico sur
| Sample_contact_city | Mexico City
| Sample_contact_zip/postal_code | 14610
| Sample_contact_country | Mexico
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025049/suppl/GSM1025049_Cas_Control1_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE41827
| Sample_data_row_count | 54675
| |
|
GSM1025050 | GPL570 |
|
HeLa control, biological rep2
|
Untreated HeLa cells
|
cell line: cervical cancer (HeLa) cells
agent: control
|
Gene expression data from untreated Hela cells.
Cas_Control-2
|
Sample_geo_accession | GSM1025050
| Sample_status | Public on Oct 31 2012
| Sample_submission_date | Oct 24 2012
| Sample_last_update_date | Oct 31 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
| Sample_growth_protocol_ch1 | HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
| Sample_hyb_protocol | Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
| Sample_scan_protocol | Scanning procedure was carried out as described by Affymetrix, Santa Clara.
| Sample_data_processing | Data pre-processing was performed by using the affy library in BioConductor running under [R].
| Sample_platform_id | GPL570
| Sample_contact_name | Karol,,Baca Lopez
| Sample_contact_email | kbacal@uaemex.mx
| Sample_contact_institute | National Institute of Genomic Medicine
| Sample_contact_address | Periferico sur
| Sample_contact_city | Mexico City
| Sample_contact_zip/postal_code | 14610
| Sample_contact_country | Mexico
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025050/suppl/GSM1025050_Cas_Control2_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE41827
| Sample_data_row_count | 54675
| |
|
GSM1025051 | GPL570 |
|
HeLa control, biological rep3
|
Untreated HeLa cells
|
cell line: cervical cancer (HeLa) cells
agent: control
|
Gene expression data from untreated Hela cells.
Cas_Control-3
|
Sample_geo_accession | GSM1025051
| Sample_status | Public on Oct 31 2012
| Sample_submission_date | Oct 24 2012
| Sample_last_update_date | Oct 31 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
| Sample_growth_protocol_ch1 | HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
| Sample_hyb_protocol | Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
| Sample_scan_protocol | Scanning procedure was carried out as described by Affymetrix, Santa Clara.
| Sample_data_processing | Data pre-processing was performed by using the affy library in BioConductor running under [R].
| Sample_platform_id | GPL570
| Sample_contact_name | Karol,,Baca Lopez
| Sample_contact_email | kbacal@uaemex.mx
| Sample_contact_institute | National Institute of Genomic Medicine
| Sample_contact_address | Periferico sur
| Sample_contact_city | Mexico City
| Sample_contact_zip/postal_code | 14610
| Sample_contact_country | Mexico
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025051/suppl/GSM1025051_Cas_Control3_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE41827
| Sample_data_row_count | 54675
| |
|
GSM1025052 | GPL570 |
|
HeLa treated with Cas-II-gly, biological rep1
|
HeLa cells treated with Cas-II-gly
|
cell line: cervical cancer (HeLa) cells
agent: Cas II-gly
|
Gene expression data from HeLa cells treated with Cas-II-gly.
Cas_II-gly-1
|
Sample_geo_accession | GSM1025052
| Sample_status | Public on Oct 31 2012
| Sample_submission_date | Oct 24 2012
| Sample_last_update_date | Oct 31 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
| Sample_growth_protocol_ch1 | HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
| Sample_hyb_protocol | Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
| Sample_scan_protocol | Scanning procedure was carried out as described by Affymetrix, Santa Clara.
| Sample_data_processing | Data pre-processing was performed by using the affy library in BioConductor running under [R].
| Sample_platform_id | GPL570
| Sample_contact_name | Karol,,Baca Lopez
| Sample_contact_email | kbacal@uaemex.mx
| Sample_contact_institute | National Institute of Genomic Medicine
| Sample_contact_address | Periferico sur
| Sample_contact_city | Mexico City
| Sample_contact_zip/postal_code | 14610
| Sample_contact_country | Mexico
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025052/suppl/GSM1025052_Cas_II-gly1_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE41827
| Sample_data_row_count | 54675
| |
|
GSM1025053 | GPL570 |
|
HeLa treated with Cas-II-gly, biological rep2
|
HeLa cells treated with Cas-II-gly
|
cell line: cervical cancer (HeLa) cells
agent: Cas II-gly
|
Gene expression data from HeLa cells treated with Cas-II-gly.
Cas_II-gly-2
|
Sample_geo_accession | GSM1025053
| Sample_status | Public on Oct 31 2012
| Sample_submission_date | Oct 24 2012
| Sample_last_update_date | Oct 31 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
| Sample_growth_protocol_ch1 | HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
| Sample_hyb_protocol | Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
| Sample_scan_protocol | Scanning procedure was carried out as described by Affymetrix, Santa Clara.
| Sample_data_processing | Data pre-processing was performed by using the affy library in BioConductor running under [R].
| Sample_platform_id | GPL570
| Sample_contact_name | Karol,,Baca Lopez
| Sample_contact_email | kbacal@uaemex.mx
| Sample_contact_institute | National Institute of Genomic Medicine
| Sample_contact_address | Periferico sur
| Sample_contact_city | Mexico City
| Sample_contact_zip/postal_code | 14610
| Sample_contact_country | Mexico
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025053/suppl/GSM1025053_Cas_II-gly2_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE41827
| Sample_data_row_count | 54675
| |
|
GSM1025054 | GPL570 |
|
HeLa treated with Cas-II-gly, biological rep3
|
HeLa cells treated with Cas-II-gly
|
cell line: cervical cancer (HeLa) cells
agent: Cas II-gly
|
Gene expression data from HeLa cells treated with Cas-II-gly.
Cas_II-gly-3
|
Sample_geo_accession | GSM1025054
| Sample_status | Public on Oct 31 2012
| Sample_submission_date | Oct 24 2012
| Sample_last_update_date | Oct 31 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were treated with Cas II-gly in 6-well microplates by 6h. In order to diminish biological variability, pools of 3 wells were made and combined into a single sample before proceeding to chip hybridization.
| Sample_growth_protocol_ch1 | HeLa cells were mantained at room temperature in 5% CO2 under sterile conditions in DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma).
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA isolated using AllPrep DNA-RNA Mini Kit 50.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Affymetrix, Santa Clara CA Inc. protocol from 400 ng total RNA.
| Sample_hyb_protocol | Hybridization to Affymetrix U133plus2 GeneChips, staining and washing were carried out as described (Affymetrix, Santa Clara).
| Sample_scan_protocol | Scanning procedure was carried out as described by Affymetrix, Santa Clara.
| Sample_data_processing | Data pre-processing was performed by using the affy library in BioConductor running under [R].
| Sample_platform_id | GPL570
| Sample_contact_name | Karol,,Baca Lopez
| Sample_contact_email | kbacal@uaemex.mx
| Sample_contact_institute | National Institute of Genomic Medicine
| Sample_contact_address | Periferico sur
| Sample_contact_city | Mexico City
| Sample_contact_zip/postal_code | 14610
| Sample_contact_country | Mexico
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025054/suppl/GSM1025054_Cas_II-gly3_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE41827
| Sample_data_row_count | 54675
| |
|
|
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