Search results for the GEO ID: GSE41839 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1025390 | GPL1261 |
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LT-HSC Zbtb7a F/+ Mx1-Cre+ #1
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LT-HSCs of control mouse
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strain/background: C57BL/6
genotype/variation: Zbtb7a Flox/+ Mx1-Cre+ (LRF heterozygous)
age: 4 weeks
cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-)
time point: 9 days after 1st pIpC injection
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B654_MG430 2-(1_2823F+)
Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
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Sample_geo_accession | GSM1025390
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Oct 25 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
| Sample_growth_protocol_ch1 | N/A
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
| Sample_hyb_protocol | Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
| Sample_scan_protocol | Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
| Sample_data_processing | The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahiro,,Maeda
| Sample_contact_email | tmaeda@partners.org
| Sample_contact_institute | Brigham and Women's Hospital
| Sample_contact_address | One Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025390/suppl/GSM1025390_B654_Cont.CEL.gz
| Sample_series_id | GSE41839
| Sample_data_row_count | 45101
| |
|
GSM1025391 | GPL1261 |
|
LT-HSC Zbtb7a F/+ Mx1-Cre+ #2
|
LT-HSCs of control mouse
|
strain/background: C57BL/6
genotype/variation: Zbtb7a Flox/+ Mx1-Cre+ (LRF heterozygous)
age: 4 weeks
cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-)
time point: 9 days after 1st pIpC injection
|
B655_MG430 2-(2_2849F+)
Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
|
Sample_geo_accession | GSM1025391
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Oct 25 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
| Sample_growth_protocol_ch1 | N/A
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
| Sample_hyb_protocol | Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
| Sample_scan_protocol | Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
| Sample_data_processing | The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahiro,,Maeda
| Sample_contact_email | tmaeda@partners.org
| Sample_contact_institute | Brigham and Women's Hospital
| Sample_contact_address | One Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025391/suppl/GSM1025391_B655_Cont.CEL.gz
| Sample_series_id | GSE41839
| Sample_data_row_count | 45101
| |
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GSM1025392 | GPL1261 |
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LT-HSC Zbtb7a F/F Mx1-Cre+ #1
|
LT-HSCs of LRF knockout mouse
|
strain/background: C57BL/6
genotype/variation: Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)
age: 4 weeks
cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-)
time point: 9 days after 1st pIpC injection
|
B656_MG430 2-(4_2846FF)
Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
|
Sample_geo_accession | GSM1025392
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Oct 25 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
| Sample_growth_protocol_ch1 | N/A
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
| Sample_hyb_protocol | Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
| Sample_scan_protocol | Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
| Sample_data_processing | The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahiro,,Maeda
| Sample_contact_email | tmaeda@partners.org
| Sample_contact_institute | Brigham and Women's Hospital
| Sample_contact_address | One Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025392/suppl/GSM1025392_B656_KO.CEL.gz
| Sample_series_id | GSE41839
| Sample_data_row_count | 45101
| |
|
GSM1025393 | GPL1261 |
|
LT-HSC Zbtb7a F/F Mx1-Cre+ #2
|
LT-HSCs of LRF knockout mouse
|
strain/background: C57BL/6
genotype/variation: Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)
age: 4 weeks
cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-)
time point: 9 days after 1st pIpC injection
|
B657_MG430 2-(5_2950FF)
Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
|
Sample_geo_accession | GSM1025393
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Oct 25 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
| Sample_growth_protocol_ch1 | N/A
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
| Sample_hyb_protocol | Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
| Sample_scan_protocol | Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
| Sample_data_processing | The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahiro,,Maeda
| Sample_contact_email | tmaeda@partners.org
| Sample_contact_institute | Brigham and Women's Hospital
| Sample_contact_address | One Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025393/suppl/GSM1025393_B657_KO.CEL.gz
| Sample_series_id | GSE41839
| Sample_data_row_count | 45101
| |
|
GSM1025394 | GPL1261 |
|
LT-HSC Zbtb7a F/F Mx1-Cre+ #3
|
LT-HSCs of LRF knockout mouse
|
strain/background: C57BL/6
genotype/variation: Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)
age: 4 weeks
cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-)
time point: 9 days after 1st pIpC injection
|
B658_MG430 2-(6_2851+2821FF)
Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
|
Sample_geo_accession | GSM1025394
| Sample_status | Public on Oct 26 2012
| Sample_submission_date | Oct 25 2012
| Sample_last_update_date | Oct 26 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
| Sample_growth_protocol_ch1 | N/A
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
| Sample_hyb_protocol | Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
| Sample_scan_protocol | Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
| Sample_data_processing | The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
| Sample_platform_id | GPL1261
| Sample_contact_name | Takahiro,,Maeda
| Sample_contact_email | tmaeda@partners.org
| Sample_contact_institute | Brigham and Women's Hospital
| Sample_contact_address | One Blackfan Circle
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1025nnn/GSM1025394/suppl/GSM1025394_B658_KO.CEL.gz
| Sample_series_id | GSE41839
| Sample_data_row_count | 45101
| |
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