Search results for the GEO ID: GSE41914  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1027378 | GPL570 |
|
NK cells_before exercise_01
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-01-PRE
|
| Sample_geo_accession | GSM1027378
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027378/suppl/GSM1027378_NK-01-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027379 | GPL570 |
|
NK cells_after exercise_01
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-03-PEAK
|
| Sample_geo_accession | GSM1027379
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027379/suppl/GSM1027379_NK-01-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027380 | GPL570 |
|
NK cells_before exercise_03
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-03-PRE
|
| Sample_geo_accession | GSM1027380
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027380/suppl/GSM1027380_NK-03-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027381 | GPL570 |
|
NK cells_after exercise_03
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-04-PEAK
|
| Sample_geo_accession | GSM1027381
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027381/suppl/GSM1027381_NK-03-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027382 | GPL570 |
|
NK cells_before exercise_04
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-04-PRE
|
| Sample_geo_accession | GSM1027382
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027382/suppl/GSM1027382_NK-04-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027383 | GPL570 |
|
NK cells_after exercise_04
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-05-PEAK
|
| Sample_geo_accession | GSM1027383
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027383/suppl/GSM1027383_NK-04-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027384 | GPL570 |
|
NK cells_before exercise_05
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-05-PRE
|
| Sample_geo_accession | GSM1027384
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027384/suppl/GSM1027384_NK-05-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027385 | GPL570 |
|
NK cells_after exercise_05
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-06-PEAK
|
| Sample_geo_accession | GSM1027385
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027385/suppl/GSM1027385_NK-05-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027386 | GPL570 |
|
NK cells_before exercise_06
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-06-PRE
|
| Sample_geo_accession | GSM1027386
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027386/suppl/GSM1027386_NK-06-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027387 | GPL570 |
|
NK cells_after exercise_06
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-07-PEAK
|
| Sample_geo_accession | GSM1027387
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027387/suppl/GSM1027387_NK-06-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027388 | GPL570 |
|
NK cells_before exercise_07
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-07-PRE
|
| Sample_geo_accession | GSM1027388
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027388/suppl/GSM1027388_NK-07-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027389 | GPL570 |
|
NK cells_after exercise_07
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-08-PEAK
|
| Sample_geo_accession | GSM1027389
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027389/suppl/GSM1027389_NK-07-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027390 | GPL570 |
|
NK cells_before exercise_08
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-08-PRE
|
| Sample_geo_accession | GSM1027390
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027390/suppl/GSM1027390_NK-08-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027391 | GPL570 |
|
NK cells_after exercise_08
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-09-PEAK
|
| Sample_geo_accession | GSM1027391
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027391/suppl/GSM1027391_NK-08-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027392 | GPL570 |
|
NK cells_before exercise_09
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-09-PRE
|
| Sample_geo_accession | GSM1027392
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027392/suppl/GSM1027392_NK-09-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027393 | GPL570 |
|
NK cells_after exercise_09
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-11-PEAK
|
| Sample_geo_accession | GSM1027393
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027393/suppl/GSM1027393_NK-09-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027394 | GPL570 |
|
NK cells_before exercise_11
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-11-PRE
|
| Sample_geo_accession | GSM1027394
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027394/suppl/GSM1027394_NK-11-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027395 | GPL570 |
|
NK cells_after exercise_11
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-12-PEAK
|
| Sample_geo_accession | GSM1027395
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027395/suppl/GSM1027395_NK-11-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027396 | GPL570 |
|
NK cells_before exercise_12
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-12-PRE
|
| Sample_geo_accession | GSM1027396
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027396/suppl/GSM1027396_NK-12-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027397 | GPL570 |
|
NK cells_after exercise_12
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-13-PEAK
|
| Sample_geo_accession | GSM1027397
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027397/suppl/GSM1027397_NK-12-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027398 | GPL570 |
|
NK cells_before exercise_13
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-13-PRE
|
| Sample_geo_accession | GSM1027398
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027398/suppl/GSM1027398_NK-13-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027399 | GPL570 |
|
NK cells_after exercise_13
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
NK-14-PEAK
|
| Sample_geo_accession | GSM1027399
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027399/suppl/GSM1027399_NK-13-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027400 | GPL570 |
|
NK cells_before exercise_14
|
Human NK cells before exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: before-exercise
|
NK-14-PRE
|
| Sample_geo_accession | GSM1027400
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027400/suppl/GSM1027400_NK-14-PEAK.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
|
GSM1027401 | GPL570 |
|
NK cells_after exercise_14
|
Human NK cells after 20 min bout of exercise
|
gender: male
age: 20-29 yr old
status: healthy
tissue: blood
cell type: NK cells
sample collection: after-exercise
|
|
| Sample_geo_accession | GSM1027401
| | Sample_status | Public on Apr 02 2013
| | Sample_submission_date | Oct 29 2012
| | Sample_last_update_date | Apr 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Blood sample was drawn before and immediately after 20 min of exercise.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | First-strand cDNA is synthesized from 250ng of isolated total RNA using an oligo-dT primer with an attached T7 promoter sequence. After making the complementary second strand, the double-stranded cDNA is used to generate biotin-tagged cRNA from an in- vitro transcription (IVT) using T7 RNA polymerase. 15ug of the resulting biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols (Affymetrix GeneChip® IVT 3’IVT Express Kit User Manual).
| | Sample_hyb_protocol | 10 ug of this fragmented target cRNA was hybridized at 45°C with rotation for 16 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix U133 plus 2 arrays.
| | Sample_scan_protocol | The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450. Arrays were scanned using GeneChip® Scanner 3000 7G and Command Console Software v 3.2.3. to produce *.CEL intensity files.
| | Sample_data_processing | The results were analyzed using GeneSpring GX 10.0.2 Software (Agilent Technologies, Inc).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shlomit,,Radom-Aizik
| | Sample_contact_email | saizik@uci.edu
| | Sample_contact_phone | 714-456-2317
| | Sample_contact_laboratory | Pediatric Exercise Research Center
| | Sample_contact_department | Pediatrics
| | Sample_contact_institute | University of California, Irvine
| | Sample_contact_address | 101 The City Drive
| | Sample_contact_city | Orange
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 92868
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1027nnn/GSM1027401/suppl/GSM1027401_NK-14-PRE.CEL.gz
| | Sample_series_id | GSE41914
| | Sample_series_id | GSE41915
| | Sample_data_row_count | 54675
| | |
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