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Search results for the GEO ID: GSE4217
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GSM ID
GPL ID
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Title
Source name
Description
Characteristics
GSM96262
GPL570
HFF2_T1_R1, Monolayer Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96263
GPL570
HFF2_T1_R2, Monolayer Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96264
GPL570
HFF2_T1_R3, Monolayer Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96265
GPL570
HFF2_T2_R1, Spheroid Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96266
GPL570
HFF2_T2_R2, Spheroid Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96267
GPL570
HFF2_T2_R3, Spheroid Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96268
GPL570
HFF2_T3_R1, Spheroid Recovery Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96269
GPL570
HFF2_T3_R2, Spheroid Recovery Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96270
GPL570
HFF2_T3_R3, Spheroid Recovery Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96271
GPL570
HFF2_T4_R1, Monolayer Recovery Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96272
GPL570
HFF2_T4_R2, Monolayer Recovery Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96273
GPL570
HFF2_T4_R3, Monolayer Recovery Human Foreskin Fibroblast (HFF2) Primary Cells 60% confluent human foreskin fibroblast (HFF2) primary cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 15% FBS supplemented DMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence). All samples were conducted in triplicate. Samples from the two week arrest were removed from storage and supplemented with DMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
 
 
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