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Search results for the GEO ID: GSE4218
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GSM ID
GPL ID
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Title
Source name
Description
Characteristics
GSM96274
GPL570
T98G_T1_R1, Monolayer Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96275
GPL570
T98G_T1_R2, Monolayer Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96276
GPL570
T98G_T1_R3, Monolayer Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96277
GPL570
T98G_T2_R1, Spheroid Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96278
GPL570
T98G_T2_R2, Spheroid Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96279
GPL570
T98G_T2_R3, Spheroid Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96280
GPL570
T98G_T3_R1, Spheroid Recovery Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96281
GPL570
T98G_T3_R2, Spheroid Recovery Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96282
GPL570
T98G_T3_R3, Spheroid Recovery Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96283
GPL570
T98G_T4_R1, Monolayer Recovery Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96284
GPL570
T98G_T4_R2, Monolayer Recovery Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
GSM96285
GPL570
T98G_T4_R3, Monolayer Recovery Human T98G Glioma Cancer Cells 60% confluent human T98G glioma cancer cells were split into suspension over agarose to form spheroids and grown for an additional 4 days in suspension over agarose prepared with 10% FBS and 5% non essential amino acids supplemented EMEM. Following growth, media was removed and the spheroids were stored on the surface of the agarose in vacuum sealed flasks for a 2 week interval at room temperature in the dark. The samples were compared against monolayer (at 60% confluence)samples. All samples were conducted intriplicate. Samples from the two week arrest were removed from storage and supplemented with EMEM (Invitrogen) containing 15% FBS (spheroid recovery sample). These cells were then allowed to grow out as adherent monolayers for 7 days (monolayer recovery sample).
 
 
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