Search results for the GEO ID: GSE42259 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1036389 | GPL570 |
|
THP1 untreated replicate 1
|
THP1 cell line
|
treatment: Untreated
cell line: THP1
cell type: Acute Myeloid leukemia cell line
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Untreated
|
Sample_geo_accession | GSM1036389
| Sample_status | Public on Jan 18 2013
| Sample_submission_date | Nov 13 2012
| Sample_last_update_date | Jan 18 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cultured on plate coated with 30 nM Dll4-Fc or vehicle for 48 hours prior to RNA extraction
| Sample_growth_protocol_ch1 | Grown in RPMI + 10% FCS + 1% Glutamax + 1% Pen/Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036389/suppl/GSM1036389_THP1_NT-1.CEL.gz
| Sample_series_id | GSE42259
| Sample_series_id | GSE42261
| Sample_data_row_count | 54675
| |
|
GSM1036390 | GPL570 |
|
THP1 untreated replicate 2
|
THP1 cell line
|
treatment: Untreated
cell line: THP1
cell type: Acute Myeloid leukemia cell line
|
Untreated
|
Sample_geo_accession | GSM1036390
| Sample_status | Public on Jan 18 2013
| Sample_submission_date | Nov 13 2012
| Sample_last_update_date | Jan 18 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cultured on plate coated with 30 nM Dll4-Fc or vehicle for 48 hours prior to RNA extraction
| Sample_growth_protocol_ch1 | Grown in RPMI + 10% FCS + 1% Glutamax + 1% Pen/Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036390/suppl/GSM1036390_THP1_NT-2.CEL.gz
| Sample_series_id | GSE42259
| Sample_series_id | GSE42261
| Sample_data_row_count | 54675
| |
|
GSM1036391 | GPL570 |
|
THP1 untreated replicate 3
|
THP1 cell line
|
treatment: Untreated
cell line: THP1
cell type: Acute Myeloid leukemia cell line
|
Untreated
|
Sample_geo_accession | GSM1036391
| Sample_status | Public on Jan 18 2013
| Sample_submission_date | Nov 13 2012
| Sample_last_update_date | Jan 18 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cultured on plate coated with 30 nM Dll4-Fc or vehicle for 48 hours prior to RNA extraction
| Sample_growth_protocol_ch1 | Grown in RPMI + 10% FCS + 1% Glutamax + 1% Pen/Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036391/suppl/GSM1036391_THP1_NT-3.CEL.gz
| Sample_series_id | GSE42259
| Sample_series_id | GSE42261
| Sample_data_row_count | 54675
| |
|
GSM1036392 | GPL570 |
|
THP1 Dll4-Fc treated replicate 1
|
THP1 cell line
|
treatment: 60nM Dll4-Fc treated
cell line: THP1
cell type: Acute Myeloid leukemia cell line
|
60nM Dll4-Fc treated
|
Sample_geo_accession | GSM1036392
| Sample_status | Public on Jan 18 2013
| Sample_submission_date | Nov 13 2012
| Sample_last_update_date | Jan 18 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cultured on plate coated with 30 nM Dll4-Fc or vehicle for 48 hours prior to RNA extraction
| Sample_growth_protocol_ch1 | Grown in RPMI + 10% FCS + 1% Glutamax + 1% Pen/Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036392/suppl/GSM1036392_THP1_Dll4Fc-1.CEL.gz
| Sample_series_id | GSE42259
| Sample_series_id | GSE42261
| Sample_data_row_count | 54675
| |
|
GSM1036393 | GPL570 |
|
THP1 Dll4-Fc treated replicate 2
|
THP1 cell line
|
treatment: 60nM Dll4-Fc treated
cell line: THP1
cell type: Acute Myeloid leukemia cell line
|
60nM Dll4-Fc treated
|
Sample_geo_accession | GSM1036393
| Sample_status | Public on Jan 18 2013
| Sample_submission_date | Nov 13 2012
| Sample_last_update_date | Jan 18 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cultured on plate coated with 30 nM Dll4-Fc or vehicle for 48 hours prior to RNA extraction
| Sample_growth_protocol_ch1 | Grown in RPMI + 10% FCS + 1% Glutamax + 1% Pen/Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036393/suppl/GSM1036393_THP1_Dll4Fc-2.CEL.gz
| Sample_series_id | GSE42259
| Sample_series_id | GSE42261
| Sample_data_row_count | 54675
| |
|
GSM1036394 | GPL570 |
|
THP1 Dll4-Fc treated replicate 3
|
THP1 cell line
|
treatment: 60nM Dll4-Fc treated
cell line: THP1
cell type: Acute Myeloid leukemia cell line
|
60nM Dll4-Fc treated
|
Sample_geo_accession | GSM1036394
| Sample_status | Public on Jan 18 2013
| Sample_submission_date | Nov 13 2012
| Sample_last_update_date | Jan 18 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | cultured on plate coated with 30 nM Dll4-Fc or vehicle for 48 hours prior to RNA extraction
| Sample_growth_protocol_ch1 | Grown in RPMI + 10% FCS + 1% Glutamax + 1% Pen/Strep
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
| Sample_hyb_protocol | 3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix)
| Sample_scan_protocol | Affymetrix 7G GeneArray scanner
| Sample_data_processing | Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
| Sample_platform_id | GPL570
| Sample_contact_name | Camille,,Lobry
| Sample_contact_email | camille.lobry@nyumc.org
| Sample_contact_laboratory | Aifantis
| Sample_contact_department | Cancer Institute/Pathology
| Sample_contact_institute | NYULMC
| Sample_contact_address | 550 First Avenue
| Sample_contact_city | New York
| Sample_contact_state | NY
| Sample_contact_zip/postal_code | 10016
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036394/suppl/GSM1036394_THP1_Dll4Fc-3.CEL.gz
| Sample_series_id | GSE42259
| Sample_series_id | GSE42261
| Sample_data_row_count | 54675
| |
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