Result

Search results for the GEO ID: GSE42260

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1036395

GPL1261

MLL-AF9 LGMP WT replicate1

Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 mouse

cell type: Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells gender: male genotype: MLL-AF9 EF1αwt/wt ROSAwt/CreERT2

6 days after tamoxifen last injection

Sample_geo_accession	GSM1036395
Sample_status	Public on Jan 18 2013
Sample_submission_date	Nov 13 2012
Sample_last_update_date	Jan 18 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	12 week old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 or MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6days after last injection. LGMP were flow purified for RNA extraction.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
Sample_hyb_protocol	3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
Sample_scan_protocol	Affymetrix 7G GeneArray scanner
Sample_data_processing	Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
Sample_platform_id	GPL1261
Sample_contact_name	Camille,,Lobry
Sample_contact_email	camille.lobry@nyumc.org
Sample_contact_laboratory	Aifantis
Sample_contact_department	Cancer Institute/Pathology
Sample_contact_institute	NYULMC
Sample_contact_address	550 First Avenue
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10016
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036395/suppl/GSM1036395_MLL-AF9_WT-1.CEL.gz
Sample_series_id	GSE42260
Sample_series_id	GSE42261
Sample_data_row_count	45101

GSM1036396

GPL1261

MLL-AF9 LGMP WT replicate2

Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 mouse

cell type: Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells gender: male genotype: MLL-AF9 EF1αwt/wt ROSAwt/CreERT2

6 days after tamoxifen last injection

Sample_geo_accession	GSM1036396
Sample_status	Public on Jan 18 2013
Sample_submission_date	Nov 13 2012
Sample_last_update_date	Jan 18 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	12 week old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 or MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6days after last injection. LGMP were flow purified for RNA extraction.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
Sample_hyb_protocol	3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
Sample_scan_protocol	Affymetrix 7G GeneArray scanner
Sample_data_processing	Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
Sample_platform_id	GPL1261
Sample_contact_name	Camille,,Lobry
Sample_contact_email	camille.lobry@nyumc.org
Sample_contact_laboratory	Aifantis
Sample_contact_department	Cancer Institute/Pathology
Sample_contact_institute	NYULMC
Sample_contact_address	550 First Avenue
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10016
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036396/suppl/GSM1036396_MLL-AF9_WT-2.CEL.gz
Sample_series_id	GSE42260
Sample_series_id	GSE42261
Sample_data_row_count	45101

GSM1036397

GPL1261

MLL-AF9 LGMP WT replicate3

Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 mouse

cell type: Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells gender: male genotype: MLL-AF9 EF1αwt/wt ROSAwt/CreERT2

6 days after tamoxifen last injection

Sample_geo_accession	GSM1036397
Sample_status	Public on Jan 18 2013
Sample_submission_date	Nov 13 2012
Sample_last_update_date	Jan 18 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	12 week old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 or MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6days after last injection. LGMP were flow purified for RNA extraction.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
Sample_hyb_protocol	3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
Sample_scan_protocol	Affymetrix 7G GeneArray scanner
Sample_data_processing	Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
Sample_platform_id	GPL1261
Sample_contact_name	Camille,,Lobry
Sample_contact_email	camille.lobry@nyumc.org
Sample_contact_laboratory	Aifantis
Sample_contact_department	Cancer Institute/Pathology
Sample_contact_institute	NYULMC
Sample_contact_address	550 First Avenue
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10016
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036397/suppl/GSM1036397_MLL-AF9_WT-3.CEL.gz
Sample_series_id	GSE42260
Sample_series_id	GSE42261
Sample_data_row_count	45101

GSM1036398

GPL1261

MLL-AF9 LGMP N1-IC+ replicate 1

Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells from MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mouse

cell type: Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells gender: female genotype: MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2

6 days after tamoxifen last injection

Sample_geo_accession	GSM1036398
Sample_status	Public on Jan 18 2013
Sample_submission_date	Nov 13 2012
Sample_last_update_date	Jan 18 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	12 week old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 or MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6days after last injection. LGMP were flow purified for RNA extraction.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
Sample_hyb_protocol	3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
Sample_scan_protocol	Affymetrix 7G GeneArray scanner
Sample_data_processing	Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
Sample_platform_id	GPL1261
Sample_contact_name	Camille,,Lobry
Sample_contact_email	camille.lobry@nyumc.org
Sample_contact_laboratory	Aifantis
Sample_contact_department	Cancer Institute/Pathology
Sample_contact_institute	NYULMC
Sample_contact_address	550 First Avenue
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10016
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036398/suppl/GSM1036398_MLL-AF9_N1-IC-1.CEL.gz
Sample_series_id	GSE42260
Sample_series_id	GSE42261
Sample_data_row_count	45101

GSM1036399

GPL1261

MLL-AF9 LGMP N1-IC+ replicate 2

Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells from MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mouse

cell type: Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells gender: female genotype: MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2

6 days after tamoxifen last injection

Sample_geo_accession	GSM1036399
Sample_status	Public on Jan 18 2013
Sample_submission_date	Nov 13 2012
Sample_last_update_date	Jan 18 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	12 week old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 or MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6days after last injection. LGMP were flow purified for RNA extraction.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
Sample_hyb_protocol	3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
Sample_scan_protocol	Affymetrix 7G GeneArray scanner
Sample_data_processing	Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
Sample_platform_id	GPL1261
Sample_contact_name	Camille,,Lobry
Sample_contact_email	camille.lobry@nyumc.org
Sample_contact_laboratory	Aifantis
Sample_contact_department	Cancer Institute/Pathology
Sample_contact_institute	NYULMC
Sample_contact_address	550 First Avenue
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10016
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036399/suppl/GSM1036399_MLL-AF9_N1-IC-2.CEL.gz
Sample_series_id	GSE42260
Sample_series_id	GSE42261
Sample_data_row_count	45101

GSM1036400

GPL1261

MLL-AF9 LGMP N1-IC+ replicate 3

Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells from MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mouse

cell type: Lineage negative, cKit+, Sca1-, YFP+, CD34+, FcgRII/III+ bone marrow cells gender: female genotype: MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2

6 days after tamoxifen last injection

Sample_geo_accession	GSM1036400
Sample_status	Public on Jan 18 2013
Sample_submission_date	Nov 13 2012
Sample_last_update_date	Jan 18 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	12 week old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1αwt/wt ROSAwt/CreERT2 or MLL-AF9 EF1αwt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6days after last injection. LGMP were flow purified for RNA extraction.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
Sample_label_ch1	biotin
Sample_label_protocol_ch1	5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
Sample_hyb_protocol	3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
Sample_scan_protocol	Affymetrix 7G GeneArray scanner
Sample_data_processing	Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
Sample_platform_id	GPL1261
Sample_contact_name	Camille,,Lobry
Sample_contact_email	camille.lobry@nyumc.org
Sample_contact_laboratory	Aifantis
Sample_contact_department	Cancer Institute/Pathology
Sample_contact_institute	NYULMC
Sample_contact_address	550 First Avenue
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10016
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036400/suppl/GSM1036400_MLL-AF9_N1-IC-3.CEL.gz
Sample_series_id	GSE42260
Sample_series_id	GSE42261
Sample_data_row_count	45101

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