Search results for the GEO ID: GSE42270  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1036563 | GPL570 |
|
HPDE-2D monolayer-1
|
HPDE-2D monolayer
|
cell line: HPDE
cell type: immortalized pancreatic epithelial cells
culture type: 2D monolayer
|
HPDE,2D,duplicate data,01
|
| Sample_geo_accession | GSM1036563
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036563/suppl/GSM1036563_HPDE-2D-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036563/suppl/GSM1036563_HPDE-2D-1.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036564 | GPL570 |
|
HPDE-2D monolayer-2
|
HPDE-2D monolayer
|
cell line: HPDE
cell type: immortalized pancreatic epithelial cells
culture type: 2D monolayer
|
HPDE,2D,duplicate data,02
|
| Sample_geo_accession | GSM1036564
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036564/suppl/GSM1036564_HPDE-2D-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036564/suppl/GSM1036564_HPDE-2D-2.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036565 | GPL570 |
|
HPDE-3D cluster-1
|
HPDE-3D cluster
|
cell line: HPDE
cell type: immortalized pancreatic epithelial cells
culture type: 3D cluster
|
HPDE,3D culture for 2day,duplicate data,01
|
| Sample_geo_accession | GSM1036565
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036565/suppl/GSM1036565_HPDE-3Dcluster-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036565/suppl/GSM1036565_HPDE-3Dcluster-1.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036566 | GPL570 |
|
HPDE-3D cluster-2
|
HPDE-3D cluster
|
cell line: HPDE
cell type: immortalized pancreatic epithelial cells
culture type: 3D cluster
|
HPDE,3D culture for 2day,duplicate data,02
|
| Sample_geo_accession | GSM1036566
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036566/suppl/GSM1036566_HPDE-3Dcluster-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036566/suppl/GSM1036566_HPDE-3Dcluster-2.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036567 | GPL570 |
|
HPDE-3D tubules-1
|
HPDE-3D tubules
|
cell line: HPDE
cell type: immortalized pancreatic epithelial cells
culture type: 3D tubules
|
HPDE,3D culture for 6day,duplicate data,01
|
| Sample_geo_accession | GSM1036567
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036567/suppl/GSM1036567_HPDE-3Dtubules-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036567/suppl/GSM1036567_HPDE-3Dtubules-1.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036568 | GPL570 |
|
HPDE-3D tubules-2
|
HPDE-3D tubules
|
cell line: HPDE
cell type: immortalized pancreatic epithelial cells
culture type: 3D tubules
|
HPDE,3D culture for 6day,duplicate data,02
|
| Sample_geo_accession | GSM1036568
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036568/suppl/GSM1036568_HPDE-3Dtubules-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036568/suppl/GSM1036568_HPDE-3Dtubules-2.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036569 | GPL570 |
|
PANC-1-2D monolayer-1
|
PANC-1-2D monolayer
|
cell line: PANC-1
cell type: pancreatic cancer cells
culture type: 2D monolayer
|
PANC-1,2D,duplicate data,01
|
| Sample_geo_accession | GSM1036569
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036569/suppl/GSM1036569_PANC-1-2D-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036569/suppl/GSM1036569_PANC-1-2D-1.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036570 | GPL570 |
|
PANC-1-2D monolayer-2
|
PANC-1-2D monolayer
|
cell line: PANC-1
cell type: pancreatic cancer cells
culture type: 2D monolayer
|
PANC-1,2D,duplicate data,02
|
| Sample_geo_accession | GSM1036570
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036570/suppl/GSM1036570_PANC-1-2D-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036570/suppl/GSM1036570_PANC-1-2D-2.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036571 | GPL570 |
|
PANC-1-3D cluster-1
|
PANC-1-3D cluster
|
cell line: PANC-1
cell type: pancreatic cancer cells
culture type: 3D cluster
|
PANC-1,3D culture for 2day,duplicate data,01
|
| Sample_geo_accession | GSM1036571
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036571/suppl/GSM1036571_PANC-1-3Dcluster-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036571/suppl/GSM1036571_PANC-1-3Dcluster-1.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036572 | GPL570 |
|
PANC-1-3D cluster-2
|
PANC-1-3D cluster
|
cell line: PANC-1
cell type: pancreatic cancer cells
culture type: 3D cluster
|
PANC-1,3D culture for 2day,duplicate data,02
|
| Sample_geo_accession | GSM1036572
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036572/suppl/GSM1036572_PANC-1-3Dcluster-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036572/suppl/GSM1036572_PANC-1-3Dcluster-2.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
| | |
|
GSM1036573 | GPL570 |
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PANC-1-3D spheroid-1
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PANC-1-3D spheroid
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cell line: PANC-1
cell type: pancreatic cancer cells
culture type: 3D spheroid
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PANC-1,3D culture for 6day,duplicate data,01
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| Sample_geo_accession | GSM1036573
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036573/suppl/GSM1036573_PANC-1-3Dspheroid-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036573/suppl/GSM1036573_PANC-1-3Dspheroid-1.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
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GSM1036574 | GPL570 |
|
PANC-1-3D spheroid-2
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PANC-1-3D spheroid
|
cell line: PANC-1
cell type: pancreatic cancer cells
culture type: 3D spheroid
|
PANC-1,3D culture for 6day,duplicate data,02
|
| Sample_geo_accession | GSM1036574
| | Sample_status | Public on Nov 15 2012
| | Sample_submission_date | Nov 14 2012
| | Sample_last_update_date | Nov 15 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | no treatment
| | Sample_growth_protocol_ch1 | HPDE cells were maintained in Keratinocyte-SFM (Sigma-Aldrich); PANC-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and antibiotics
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA samples were extracted from cells or organoids using TRIZOL (Sigma) and then purified using an RNeasy mini-kit and a DNase treatment (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1ug total RNA (Genechip 3'-IVT express kit, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A 2.0 Plus GeneChip. GeneChips were washed and stained in the GeneChip®Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip®Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Wei-Yu,,Wang
| | Sample_contact_email | littlebear5003@gmail.com
| | Sample_contact_institute | National Health Research Institutes
| | Sample_contact_address | 2F., No. 367, Shengli Road
| | Sample_contact_city | Tainan City
| | Sample_contact_zip/postal_code | 70456
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036574/suppl/GSM1036574_PANC-1-3Dspheroid-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1036nnn/GSM1036574/suppl/GSM1036574_PANC-1-3Dspheroid-2.CHP.gz
| | Sample_series_id | GSE42270
| | Sample_data_row_count | 54675
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