Result

Search results for the GEO ID: GSE42299

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GSM ID

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Title

Source name

Description

Characteristics

GSM1037396

GPL1261

C2C12_GFP_rep1

C2C12 mouse myotube, GFP adenovirus

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP treatment: none

GFP 1

Sample_geo_accession	GSM1037396
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037396/suppl/GSM1037396_GFP_1.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037397

GPL1261

C2C12_GFP_rep2

C2C12 mouse myotube, GFP adenovirus

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP treatment: none

GFP 2

Sample_geo_accession	GSM1037397
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037397/suppl/GSM1037397_GFP_2.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037398

GPL1261

C2C12_GFP-PGC-1α_rep1

C2C12 mouse myotube, GFP-PGC-1α adenovirus

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP-PGC-1α treatment: none

PGC 1

Sample_geo_accession	GSM1037398
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037398/suppl/GSM1037398_PGC_1.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037399

GPL1261

C2C12_GFP-PGC-1α_rep2

C2C12 mouse myotube, GFP-PGC-1α adenovirus

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP-PGC-1α treatment: none

PGC 2

Sample_geo_accession	GSM1037399
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037399/suppl/GSM1037399_PGC_2.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037400

GPL1261

C2C12_GFP-PGC-1α+DFO_rep1

C2C12 mouse myotube, GFP-PGC-1α adenovirus, DFO treatment

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP-PGC-1α treatment: deferoxamine (DFO)

PGC+DFO 1

Sample_geo_accession	GSM1037400
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037400/suppl/GSM1037400_PGC_DFO_1.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037401

GPL1261

C2C12_GFP-PGC-1α+DFO_rep2

C2C12 mouse myotube, GFP-PGC-1α adenovirus, DFO treatment

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP-PGC-1α treatment: deferoxamine (DFO)

PGC+DFO 2

Sample_geo_accession	GSM1037401
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037401/suppl/GSM1037401_PGC_DFO_2.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037402

GPL1261

C2C12_GFP+DFO_rep1

C2C12 mouse myotube, GFP adenovirus, DFO treatment

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP treatment: deferoxamine (DFO)

DFO 1

Sample_geo_accession	GSM1037402
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037402/suppl/GSM1037402_DFO_1.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

GSM1037403

GPL1261

C2C12_GFP+DFO_rep2

C2C12 mouse myotube, GFP adenovirus, DFO treatment

cell line: C2C12 morphology: myotube adenovirus overexpression construct: GFP treatment: deferoxamine (DFO)

DFO 2

Sample_geo_accession	GSM1037403
Sample_status	Public on Dec 10 2012
Sample_submission_date	Nov 14 2012
Sample_last_update_date	Dec 10 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	After 3 days of differentiation, myotubes were infected with adenovirus containing overexpression constructs for either GFP or GFP-PGC-1α ± treatment with 100 µM DFO. The cells were then incubated for 3 days, after which RNA was extracted.
Sample_growth_protocol_ch1	C2C12 mouse myoblasts were maintained in high glucose DMEM with 10% fetal bovine serum (FBS) and 1 × penicillin-streptomycin at 37°C and 5% CO2. To induce differentiation, the myoblasts were grown to near confluence then switched to media containing 2% dialyzed FBS.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with an RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Sample_hyb_protocol	Following fragmentation, cRNA were hybridized on Affymetrix Mouse 430 2.0 Arrays using standard Affymetrix procedures.
Sample_scan_protocol	GeneChips were scanned using the GCS 3000 from Affymetrix.
Sample_data_processing	Files were processed by dCHIP using model-based expression with mismatch probe background subtraction, quantile normalization and running median smoothing.
Sample_platform_id	GPL1261
Sample_contact_name	David,J.,Pagliarini
Sample_contact_email	pagliarini@wisc.edu
Sample_contact_phone	608-890-3254
Sample_contact_fax	608-262-3453
Sample_contact_laboratory	Pagliarini
Sample_contact_department	Biochemistry
Sample_contact_institute	University of Wisconsin-Madison
Sample_contact_address	433 Babcock Dr.
Sample_contact_city	Madison
Sample_contact_state	WI
Sample_contact_zip/postal_code	53706
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037403/suppl/GSM1037403_DFO_2.CEL.gz
Sample_series_id	GSE42299
Sample_data_row_count	45101

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