Search results for the GEO ID: GSE42320 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1037639 | GPL570 |
|
IKKe B2 shRNA, biological rep1
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B2 shRNA
|
Sample_geo_accession | GSM1037639
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037639/suppl/GSM1037639_Lidia_B2_1_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037640 | GPL570 |
|
IKKe B2 shRNA, biological rep2
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B2 shRNA
|
Sample_geo_accession | GSM1037640
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037640/suppl/GSM1037640_Lidia_B2_2_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037641 | GPL570 |
|
IKKe B2 shRNA, biological rep3
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B2 shRNA
|
Sample_geo_accession | GSM1037641
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037641/suppl/GSM1037641_Lidia_B2_3_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037642 | GPL570 |
|
IKKe B2 shRNA, biological rep4
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B2 shRNA
|
Sample_geo_accession | GSM1037642
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037642/suppl/GSM1037642_Lidia_B2_4_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037643 | GPL570 |
|
IKKe B2 shRNA, biological rep5
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B2 shRNA
|
Sample_geo_accession | GSM1037643
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037643/suppl/GSM1037643_Lidia_B2_5_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037644 | GPL570 |
|
IKKe B2 shRNA, biological rep6
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B2 shRNA
|
Sample_geo_accession | GSM1037644
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037644/suppl/GSM1037644_Lidia_B2_6_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037645 | GPL570 |
|
IKKe B6 shRNA, biological rep1
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B6 shRNA
|
Sample_geo_accession | GSM1037645
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037645/suppl/GSM1037645_Lidia_B6_1_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037646 | GPL570 |
|
IKKe B6 shRNA, biological rep2
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B6 shRNA
|
Sample_geo_accession | GSM1037646
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037646/suppl/GSM1037646_Lidia_B6_2_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037647 | GPL570 |
|
IKKe B6 shRNA, biological rep3
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B6 shRNA
|
Sample_geo_accession | GSM1037647
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037647/suppl/GSM1037647_Lidia_B6_3_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037648 | GPL570 |
|
IKKe B6 shRNA, biological rep4
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B6 shRNA
|
Sample_geo_accession | GSM1037648
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037648/suppl/GSM1037648_Lidia_B6_4_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037649 | GPL570 |
|
IKKe B6 shRNA, biological rep5
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B6 shRNA
|
Sample_geo_accession | GSM1037649
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037649/suppl/GSM1037649_Lidia_B6_5_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037650 | GPL570 |
|
IKKe B6 shRNA, biological rep6
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: IKKe knockdown
|
Gene expression data from IKKe B6 shRNA
|
Sample_geo_accession | GSM1037650
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037650/suppl/GSM1037650_Lidia_B6_6_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037651 | GPL570 |
|
SC4 neg shRNA, biological rep1
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: control
|
Gene expression data from IKKe SC4 shRNA
|
Sample_geo_accession | GSM1037651
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037651/suppl/GSM1037651_Lidia_SC4_1_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037652 | GPL570 |
|
SC4 neg shRNA, biological rep2
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: control
|
Gene expression data from IKKe SC4 shRNA
|
Sample_geo_accession | GSM1037652
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037652/suppl/GSM1037652_Lidia_SC4_2_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037653 | GPL570 |
|
SC4 neg shRNA, biological rep3
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: control
|
Gene expression data from IKKe SC4 shRNA
|
Sample_geo_accession | GSM1037653
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037653/suppl/GSM1037653_Lidia_SC4_3_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037654 | GPL570 |
|
SC4 neg shRNA, biological rep4
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: control
|
Gene expression data from IKKe SC4 shRNA
|
Sample_geo_accession | GSM1037654
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037654/suppl/GSM1037654_Lidia_SC4_4_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037655 | GPL570 |
|
SC4 neg shRNA, biological rep5
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: control
|
Gene expression data from IKKe SC4 shRNA
|
Sample_geo_accession | GSM1037655
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037655/suppl/GSM1037655_Lidia_SC4_5_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
GSM1037656 | GPL570 |
|
SC4 neg shRNA, biological rep6
|
ovarian cancer cells
|
cell type: adherent cells
cell line: Ovcar5
treatment: control
|
Gene expression data from IKKe SC4 shRNA
|
Sample_geo_accession | GSM1037656
| Sample_status | Public on Nov 16 2012
| Sample_submission_date | Nov 15 2012
| Sample_last_update_date | Nov 16 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Viral supernatants were prepared by transient transfection into a CeB packaging cell line. Two rounds of viral supernatants were applied to Ovcar5 in 6-well plates over the course of 48h, and then cells were incubated with growth medium for 24h, followed by selection with 2ug/ml puromycin for 7 days.
| Sample_growth_protocol_ch1 | RPMI plus 10% fetal bovine serum (Hyclone, Pittsburg, PA) and standard antibiotics.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using Trizol (Invitrogen, Carlsbad, CA) according to manufacturer protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit, following manufacturer protocol
| Sample_hyb_protocol | Hybridized to Affymetrix H133 Plus 2.0 gene chips, following manufacturer protocol
| Sample_scan_protocol | Scanned by Affymetrix GeneChip scanner 3000, following manufacturer protocol
| Sample_data_processing | CEL files were imported into GeneSpring software 11.5 (Agilent), probe levels were normalized by the GC-RMA algorithm.
| Sample_platform_id | GPL570
| Sample_contact_name | Marianne,Kwang-Hee,Kim
| Sample_contact_email | marianne.kim@nih.gov
| Sample_contact_phone | 301-451-3333
| Sample_contact_fax | 301-480-6255
| Sample_contact_department | Medical Oncology Branch
| Sample_contact_institute | NIH/NCI
| Sample_contact_address | 10 Center Dr. 3B45
| Sample_contact_city | Bethesda
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 20892
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037656/suppl/GSM1037656_Lidia_SC4_6_HG-U133_Plus_2_.CEL.gz
| Sample_series_id | GSE42320
| Sample_data_row_count | 54675
| |
|
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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Select GSMs and click on "Add groups" |
Enter the group name here: |
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