Result

Search results for the GEO ID: GSE42327

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GPL ID

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Title

Source name

Description

Characteristics

GSM1037857

GPL570

siDsRed-1, biological rep1

HeLa cells were treated with the siRNAs for 72 hrs

treatment: siDsRed cell line: HeLa

siDsRed HeLa cells replicate 1

Sample_geo_accession	GSM1037857
Sample_status	Public on May 21 2013
Sample_submission_date	Nov 15 2012
Sample_last_update_date	May 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	HeLa cells were treated with the siRNAs for 72 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA for microarray analysis was isolated with TRIzol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer’s instructions. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChipR 3’ IVT Express Kit User Manual, P/N 702646 Rev. 1).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (GeneChip Expression Wash, Stain and Scan User Manual For Cartridge Arrays, P/N 702731 Rev. 2)
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The scanned images were analyzed using the GeneChip operating software version 1.4 (Affymetrix 690036) with Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling was used as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Daisuke,,Okuzaki
Sample_contact_email	dokuzaki@biken.osaka-u.ac.jp
Sample_contact_phone	+81-6-6875-3980
Sample_contact_department	DNA-chip Development Center for Infectious Diseases
Sample_contact_institute	RIMD, Osaka univ.
Sample_contact_address	3-1 yamadaoka
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037857/suppl/GSM1037857_GC467-01_HGU133_2_090209.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037857/suppl/GSM1037857_GC467-01_HGU133_2_090209.CHP.gz
Sample_series_id	GSE42327
Sample_data_row_count	54675

GSM1037858

GPL570

siDsRed-2, biological rep2

HeLa cells were treated with the siRNAs for 72 hrs

treatment: siDsRed cell line: HeLa

siDsRed HeLa cells replicate 2

Sample_geo_accession	GSM1037858
Sample_status	Public on May 21 2013
Sample_submission_date	Nov 15 2012
Sample_last_update_date	May 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	HeLa cells were treated with the siRNAs for 72 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA for microarray analysis was isolated with TRIzol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer’s instructions. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChipR 3’ IVT Express Kit User Manual, P/N 702646 Rev. 1).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (GeneChip Expression Wash, Stain and Scan User Manual For Cartridge Arrays, P/N 702731 Rev. 2)
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The scanned images were analyzed using the GeneChip operating software version 1.4 (Affymetrix 690036) with Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling was used as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Daisuke,,Okuzaki
Sample_contact_email	dokuzaki@biken.osaka-u.ac.jp
Sample_contact_phone	+81-6-6875-3980
Sample_contact_department	DNA-chip Development Center for Infectious Diseases
Sample_contact_institute	RIMD, Osaka univ.
Sample_contact_address	3-1 yamadaoka
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037858/suppl/GSM1037858_GC495-01_HGU133_P2_090326.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037858/suppl/GSM1037858_GC495-01_HGU133_P2_090326.CHP.gz
Sample_series_id	GSE42327
Sample_data_row_count	54675

GSM1037859

GPL570

siThoc5-1, biological rep1

HeLa cells were treated with the siRNAs for 72 hrs

treatment: siThoc5 cell line: HeLa

siThoc5 HeLa cells replicate 1

Sample_geo_accession	GSM1037859
Sample_status	Public on May 21 2013
Sample_submission_date	Nov 15 2012
Sample_last_update_date	May 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	HeLa cells were treated with the siRNAs for 72 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA for microarray analysis was isolated with TRIzol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer’s instructions. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChipR 3’ IVT Express Kit User Manual, P/N 702646 Rev. 1).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (GeneChip Expression Wash, Stain and Scan User Manual For Cartridge Arrays, P/N 702731 Rev. 2)
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The scanned images were analyzed using the GeneChip operating software version 1.4 (Affymetrix 690036) with Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling was used as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Daisuke,,Okuzaki
Sample_contact_email	dokuzaki@biken.osaka-u.ac.jp
Sample_contact_phone	+81-6-6875-3980
Sample_contact_department	DNA-chip Development Center for Infectious Diseases
Sample_contact_institute	RIMD, Osaka univ.
Sample_contact_address	3-1 yamadaoka
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037859/suppl/GSM1037859_GC467-02_HGU133_2_090209.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037859/suppl/GSM1037859_GC467-02_HGU133_2_090209.CHP.gz
Sample_series_id	GSE42327
Sample_data_row_count	54675

GSM1037860

GPL570

siThoc5-2, biological rep2

HeLa cells were treated with the siRNAs for 72 hrs

treatment: siThoc5 cell line: HeLa

siThoc5 HeLa cells replicate 2

Sample_geo_accession	GSM1037860
Sample_status	Public on May 21 2013
Sample_submission_date	Nov 15 2012
Sample_last_update_date	May 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	HeLa cells were treated with the siRNAs for 72 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA for microarray analysis was isolated with TRIzol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer’s instructions. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChipR 3’ IVT Express Kit User Manual, P/N 702646 Rev. 1).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (GeneChip Expression Wash, Stain and Scan User Manual For Cartridge Arrays, P/N 702731 Rev. 2)
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The scanned images were analyzed using the GeneChip operating software version 1.4 (Affymetrix 690036) with Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling was used as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Daisuke,,Okuzaki
Sample_contact_email	dokuzaki@biken.osaka-u.ac.jp
Sample_contact_phone	+81-6-6875-3980
Sample_contact_department	DNA-chip Development Center for Infectious Diseases
Sample_contact_institute	RIMD, Osaka univ.
Sample_contact_address	3-1 yamadaoka
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037860/suppl/GSM1037860_GC495-02_HGU133_P2_090326.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037860/suppl/GSM1037860_GC495-02_HGU133_P2_090326.CHP.gz
Sample_series_id	GSE42327
Sample_data_row_count	54675

GSM1037861

GPL570

siCFIm68-1, biological rep1

HeLa cells were treated with the siRNAs for 72 hrs

treatment: siCFIm68 cell line: HeLa

siCFIm68 HeLa cells replicate 1

Sample_geo_accession	GSM1037861
Sample_status	Public on May 21 2013
Sample_submission_date	Nov 15 2012
Sample_last_update_date	May 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	HeLa cells were treated with the siRNAs for 72 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA for microarray analysis was isolated with TRIzol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer’s instructions. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChipR 3’ IVT Express Kit User Manual, P/N 702646 Rev. 1).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (GeneChip Expression Wash, Stain and Scan User Manual For Cartridge Arrays, P/N 702731 Rev. 2)
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The scanned images were analyzed using the GeneChip operating software version 1.4 (Affymetrix 690036) with Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling was used as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Daisuke,,Okuzaki
Sample_contact_email	dokuzaki@biken.osaka-u.ac.jp
Sample_contact_phone	+81-6-6875-3980
Sample_contact_department	DNA-chip Development Center for Infectious Diseases
Sample_contact_institute	RIMD, Osaka univ.
Sample_contact_address	3-1 yamadaoka
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037861/suppl/GSM1037861_GC467-03_HGU133_2_090209.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037861/suppl/GSM1037861_GC467-03_HGU133_2_090209.CHP.gz
Sample_series_id	GSE42327
Sample_data_row_count	54675

GSM1037862

GPL570

siCFIm68-2, biological rep2

HeLa cells were treated with the siRNAs for 72 hrs

treatment: siCFIm68 cell line: HeLa

siCFIm68 HeLa cells replicate 2

Sample_geo_accession	GSM1037862
Sample_status	Public on May 21 2013
Sample_submission_date	Nov 15 2012
Sample_last_update_date	May 21 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_growth_protocol_ch1	HeLa cells were treated with the siRNAs for 72 hrs.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA for microarray analysis was isolated with TRIzol reagent (Life Technologies, Rockville, MD, USA) according to the manufacturer’s instructions. RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChipR 3’ IVT Express Kit User Manual, P/N 702646 Rev. 1).
Sample_hyb_protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (GeneChip Expression Wash, Stain and Scan User Manual For Cartridge Arrays, P/N 702731 Rev. 2)
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GCS3000 scanner.
Sample_data_processing	The scanned images were analyzed using the GeneChip operating software version 1.4 (Affymetrix 690036) with Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling was used as normalization method. The trimmed mean target intensity of each array was set to 500.
Sample_platform_id	GPL570
Sample_contact_name	Daisuke,,Okuzaki
Sample_contact_email	dokuzaki@biken.osaka-u.ac.jp
Sample_contact_phone	+81-6-6875-3980
Sample_contact_department	DNA-chip Development Center for Infectious Diseases
Sample_contact_institute	RIMD, Osaka univ.
Sample_contact_address	3-1 yamadaoka
Sample_contact_city	Suita
Sample_contact_state	Osaka
Sample_contact_zip/postal_code	565-0871
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037862/suppl/GSM1037862_GC495-03_HGU133_P2_090326.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1037nnn/GSM1037862/suppl/GSM1037862_GC495-03_HGU133_P2_090326.CHP.gz
Sample_series_id	GSE42327
Sample_data_row_count	54675

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