Search results for the GEO ID: GSE42463  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1040577 | GPL1261 |
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F9 shVector, biological rep1
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F9 shVector, biological rep1
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cell line: F9
shRNA: Vector control
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Gene expression data from F9 shVector cells, biological rep1
|
| Sample_geo_accession | GSM1040577
| | Sample_status | Public on Dec 20 2012
| | Sample_submission_date | Nov 21 2012
| | Sample_last_update_date | Dec 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lentivirus-based shRNA constructs (pLKO.1) that target mose Phf19 gene were obtained from Open Biosystems. pLKO.1-shRNA lentivirus was generated in 293 cells and used according to Open Biosystems protocols. Stable F9 cells were selected by puromycin after pLKO.1-shPhf19 and pLKO.1-shRNA vector control (shVector) lentivirus infection.
| | Sample_growth_protocol_ch1 | F9 embryonic carcinoma cells were obtained from ATCC and maintained using recommended culture conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | Hybridization signals were retrieved and normalized, followed by differential expression analysis and statistical analysis using GeneSpring Analysis Platform GX 11 (Agilent Technologies).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Rui,,Lu
| | Sample_contact_email | ruilu@med.unc.edu
| | Sample_contact_institute | UNC at Chaple Hill
| | Sample_contact_address | 450 West Drive, CB 7295
| | Sample_contact_city | Chapel Hill
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27599-7295
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040577/suppl/GSM1040577_F9_shVector-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040577/suppl/GSM1040577_F9_shVector-1.CHP.gz
| | Sample_series_id | GSE42463
| | Sample_data_row_count | 45101
| | |
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GSM1040578 | GPL1261 |
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F9 shPhf19 biological rep1
|
F9 shPhf19 biological rep1
|
cell line: F9
shRNA: shPhf19
|
Gene expression data from F9 shPhf19 cells, biological rep1
|
| Sample_geo_accession | GSM1040578
| | Sample_status | Public on Dec 20 2012
| | Sample_submission_date | Nov 21 2012
| | Sample_last_update_date | Dec 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lentivirus-based shRNA constructs (pLKO.1) that target mose Phf19 gene were obtained from Open Biosystems. pLKO.1-shRNA lentivirus was generated in 293 cells and used according to Open Biosystems protocols. Stable F9 cells were selected by puromycin after pLKO.1-shPhf19 and pLKO.1-shRNA vector control (shVector) lentivirus infection.
| | Sample_growth_protocol_ch1 | F9 embryonic carcinoma cells were obtained from ATCC and maintained using recommended culture conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | Hybridization signals were retrieved and normalized, followed by differential expression analysis and statistical analysis using GeneSpring Analysis Platform GX 11 (Agilent Technologies).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Rui,,Lu
| | Sample_contact_email | ruilu@med.unc.edu
| | Sample_contact_institute | UNC at Chaple Hill
| | Sample_contact_address | 450 West Drive, CB 7295
| | Sample_contact_city | Chapel Hill
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27599-7295
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040578/suppl/GSM1040578_F9_shPHF19-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040578/suppl/GSM1040578_F9_shPHF19-1.CHP.gz
| | Sample_series_id | GSE42463
| | Sample_data_row_count | 45101
| | |
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GSM1040579 | GPL1261 |
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F9 shVector, biological rep2
|
F9 shVector, biological rep2
|
cell line: F9
shRNA: Vector control
|
Gene expression data from F9 shVector cells, biological rep2
|
| Sample_geo_accession | GSM1040579
| | Sample_status | Public on Dec 20 2012
| | Sample_submission_date | Nov 21 2012
| | Sample_last_update_date | Dec 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lentivirus-based shRNA constructs (pLKO.1) that target mose Phf19 gene were obtained from Open Biosystems. pLKO.1-shRNA lentivirus was generated in 293 cells and used according to Open Biosystems protocols. Stable F9 cells were selected by puromycin after pLKO.1-shPhf19 and pLKO.1-shRNA vector control (shVector) lentivirus infection.
| | Sample_growth_protocol_ch1 | F9 embryonic carcinoma cells were obtained from ATCC and maintained using recommended culture conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | Hybridization signals were retrieved and normalized, followed by differential expression analysis and statistical analysis using GeneSpring Analysis Platform GX 11 (Agilent Technologies).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Rui,,Lu
| | Sample_contact_email | ruilu@med.unc.edu
| | Sample_contact_institute | UNC at Chaple Hill
| | Sample_contact_address | 450 West Drive, CB 7295
| | Sample_contact_city | Chapel Hill
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27599-7295
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040579/suppl/GSM1040579_F9_shVector-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040579/suppl/GSM1040579_F9_shVector-2.CHP.gz
| | Sample_series_id | GSE42463
| | Sample_data_row_count | 45101
| | |
|
GSM1040580 | GPL1261 |
|
F9 shPhf19, biological rep2
|
F9 shPhf19, biological rep2
|
cell line: F9
shRNA: shPhf19
|
Gene expression data from F9 shPhf19 cells, biological rep2
|
| Sample_geo_accession | GSM1040580
| | Sample_status | Public on Dec 20 2012
| | Sample_submission_date | Nov 21 2012
| | Sample_last_update_date | Dec 20 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Lentivirus-based shRNA constructs (pLKO.1) that target mose Phf19 gene were obtained from Open Biosystems. pLKO.1-shRNA lentivirus was generated in 293 cells and used according to Open Biosystems protocols. Stable F9 cells were selected by puromycin after pLKO.1-shPhf19 and pLKO.1-shRNA vector control (shVector) lentivirus infection.
| | Sample_growth_protocol_ch1 | F9 embryonic carcinoma cells were obtained from ATCC and maintained using recommended culture conditions.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol
| | Sample_hyb_protocol | Standard Affymetrix protocol
| | Sample_scan_protocol | Standard Affymetrix protocol
| | Sample_data_processing | Hybridization signals were retrieved and normalized, followed by differential expression analysis and statistical analysis using GeneSpring Analysis Platform GX 11 (Agilent Technologies).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Rui,,Lu
| | Sample_contact_email | ruilu@med.unc.edu
| | Sample_contact_institute | UNC at Chaple Hill
| | Sample_contact_address | 450 West Drive, CB 7295
| | Sample_contact_city | Chapel Hill
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27599-7295
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040580/suppl/GSM1040580_F9_shPHF19-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1040nnn/GSM1040580/suppl/GSM1040580_F9_shPHF19-2.CHP.gz
| | Sample_series_id | GSE42463
| | Sample_data_row_count | 45101
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