Result

Search results for the GEO ID: GSE42569

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GSM ID

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Title

Source name

Description

Characteristics

GSM1045312

GPL570

donor 1 control conditions

CD4+ T cells differentiated under control conditions, day7

tissue: lymphocyte

Gene expression data from naive CD4+ T cells differentiated into Th17 cells

Sample_geo_accession	GSM1045312
Sample_status	Public on Mar 07 2013
Sample_submission_date	Nov 28 2012
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	plate bound aCD3 (10µg/ml) and soluble aCD28 (1µg/ml) and soluble IL-1beta (12.5ng/ml), IL-6 (25ng/ml), IL-21 (25ng/ml), IL-23 (25ng/ml) and TGF-beta1(5ng/ml) in the presence (nacl) or absence (none) of additional 40mM NaCl in X-VIVO cell culture media (LONZA). RNA was extracted after 7 days of culture.
Sample_growth_protocol_ch1	FACS sorted naive human CD4+ T cells (CD45RA+CD45RO-CD25-CD127+) were differentiated into Th17 cells by stimulation with aCD3 and aCD28 and IL-1beta, IL-6, IL-21, IL-23 and TGF-beta1 in the presence (nacl) or absence (none) of additional 40mM NaCl. RNA was extracted after 7 days of culture.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by Trizol according to the manufacturer (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Standard 3´IVT and biotin labelling at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_hyb_protocol	Standard hybridisation at Yale Center for Genome Analysis (YCGA) for 16 hours at 45C to Affymetrix HG-U133 plus 2.0 arrays according to Affymetrix guidelines.
Sample_scan_protocol	Standard scan at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_data_processing	The image data were analyzed using Affymetrix Command Console Viewer and metric analyses was carried out according to the instructions provided by Affymetrix using the Affymetrix Expression Console and standard MAS5.0 processing at Yale Center for Genome Analysis (YCGA).
Sample_platform_id	GPL570
Sample_contact_name	Markus,,Kleinewietfeld
Sample_contact_institute	Yale
Sample_contact_address	300 George St
Sample_contact_city	New Haven
Sample_contact_zip/postal_code	06511
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045312/suppl/GSM1045312_d1_none.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045312/suppl/GSM1045312_d1_none.CHP.gz
Sample_series_id	GSE42569
Sample_data_row_count	54675

GSM1045313

GPL570

donor 1 high salt conditions

CD4+ T cells differentiated under high-salt conditions, day7

tissue: lymphocyte

Gene expression data from naive CD4+ T cells differentiated into Th17 cells in the presence of high-salt

Sample_geo_accession	GSM1045313
Sample_status	Public on Mar 07 2013
Sample_submission_date	Nov 28 2012
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	plate bound aCD3 (10µg/ml) and soluble aCD28 (1µg/ml) and soluble IL-1beta (12.5ng/ml), IL-6 (25ng/ml), IL-21 (25ng/ml), IL-23 (25ng/ml) and TGF-beta1(5ng/ml) in the presence (nacl) or absence (none) of additional 40mM NaCl in X-VIVO cell culture media (LONZA). RNA was extracted after 7 days of culture.
Sample_growth_protocol_ch1	FACS sorted naive human CD4+ T cells (CD45RA+CD45RO-CD25-CD127+) were differentiated into Th17 cells by stimulation with aCD3 and aCD28 and IL-1beta, IL-6, IL-21, IL-23 and TGF-beta1 in the presence (nacl) or absence (none) of additional 40mM NaCl. RNA was extracted after 7 days of culture.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by Trizol according to the manufacturer (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Standard 3´IVT and biotin labelling at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_hyb_protocol	Standard hybridisation at Yale Center for Genome Analysis (YCGA) for 16 hours at 45C to Affymetrix HG-U133 plus 2.0 arrays according to Affymetrix guidelines.
Sample_scan_protocol	Standard scan at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_data_processing	The image data were analyzed using Affymetrix Command Console Viewer and metric analyses was carried out according to the instructions provided by Affymetrix using the Affymetrix Expression Console and standard MAS5.0 processing at Yale Center for Genome Analysis (YCGA).
Sample_platform_id	GPL570
Sample_contact_name	Markus,,Kleinewietfeld
Sample_contact_institute	Yale
Sample_contact_address	300 George St
Sample_contact_city	New Haven
Sample_contact_zip/postal_code	06511
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045313/suppl/GSM1045313_d1_nacl.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045313/suppl/GSM1045313_d1_nacl.CHP.gz
Sample_series_id	GSE42569
Sample_data_row_count	54675

GSM1045314

GPL570

donor 2 control conditions

CD4+ T cells differentiated under control conditions, day7

tissue: lymphocyte

Gene expression data from naive CD4+ T cells differentiated into Th17 cells

Sample_geo_accession	GSM1045314
Sample_status	Public on Mar 07 2013
Sample_submission_date	Nov 28 2012
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	plate bound aCD3 (10µg/ml) and soluble aCD28 (1µg/ml) and soluble IL-1beta (12.5ng/ml), IL-6 (25ng/ml), IL-21 (25ng/ml), IL-23 (25ng/ml) and TGF-beta1(5ng/ml) in the presence (nacl) or absence (none) of additional 40mM NaCl in X-VIVO cell culture media (LONZA). RNA was extracted after 7 days of culture.
Sample_growth_protocol_ch1	FACS sorted naive human CD4+ T cells (CD45RA+CD45RO-CD25-CD127+) were differentiated into Th17 cells by stimulation with aCD3 and aCD28 and IL-1beta, IL-6, IL-21, IL-23 and TGF-beta1 in the presence (nacl) or absence (none) of additional 40mM NaCl. RNA was extracted after 7 days of culture.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by Trizol according to the manufacturer (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Standard 3´IVT and biotin labelling at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_hyb_protocol	Standard hybridisation at Yale Center for Genome Analysis (YCGA) for 16 hours at 45C to Affymetrix HG-U133 plus 2.0 arrays according to Affymetrix guidelines.
Sample_scan_protocol	Standard scan at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_data_processing	The image data were analyzed using Affymetrix Command Console Viewer and metric analyses was carried out according to the instructions provided by Affymetrix using the Affymetrix Expression Console and standard MAS5.0 processing at Yale Center for Genome Analysis (YCGA).
Sample_platform_id	GPL570
Sample_contact_name	Markus,,Kleinewietfeld
Sample_contact_institute	Yale
Sample_contact_address	300 George St
Sample_contact_city	New Haven
Sample_contact_zip/postal_code	06511
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045314/suppl/GSM1045314_d2_none.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045314/suppl/GSM1045314_d2_none.CHP.gz
Sample_series_id	GSE42569
Sample_data_row_count	54675

GSM1045315

GPL570

donor 2 high salt conditions

CD4+ T cells differentiated under high-salt conditions, day7

tissue: lymphocyte

Gene expression data from naive CD4+ T cells differentiated into Th17 cells in the presence of high-salt

Sample_geo_accession	GSM1045315
Sample_status	Public on Mar 07 2013
Sample_submission_date	Nov 28 2012
Sample_last_update_date	Mar 07 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	plate bound aCD3 (10µg/ml) and soluble aCD28 (1µg/ml) and soluble IL-1beta (12.5ng/ml), IL-6 (25ng/ml), IL-21 (25ng/ml), IL-23 (25ng/ml) and TGF-beta1(5ng/ml) in the presence (nacl) or absence (none) of additional 40mM NaCl in X-VIVO cell culture media (LONZA). RNA was extracted after 7 days of culture.
Sample_growth_protocol_ch1	FACS sorted naive human CD4+ T cells (CD45RA+CD45RO-CD25-CD127+) were differentiated into Th17 cells by stimulation with aCD3 and aCD28 and IL-1beta, IL-6, IL-21, IL-23 and TGF-beta1 in the presence (nacl) or absence (none) of additional 40mM NaCl. RNA was extracted after 7 days of culture.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted by Trizol according to the manufacturer (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Standard 3´IVT and biotin labelling at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_hyb_protocol	Standard hybridisation at Yale Center for Genome Analysis (YCGA) for 16 hours at 45C to Affymetrix HG-U133 plus 2.0 arrays according to Affymetrix guidelines.
Sample_scan_protocol	Standard scan at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
Sample_data_processing	The image data were analyzed using Affymetrix Command Console Viewer and metric analyses was carried out according to the instructions provided by Affymetrix using the Affymetrix Expression Console and standard MAS5.0 processing at Yale Center for Genome Analysis (YCGA).
Sample_platform_id	GPL570
Sample_contact_name	Markus,,Kleinewietfeld
Sample_contact_institute	Yale
Sample_contact_address	300 George St
Sample_contact_city	New Haven
Sample_contact_zip/postal_code	06511
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045315/suppl/GSM1045315_d2_nacl.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1045nnn/GSM1045315/suppl/GSM1045315_d2_nacl.CHP.gz
Sample_series_id	GSE42569
Sample_data_row_count	54675

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