Result

Search results for the GEO ID: GSE42753

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1049430

GPL1261

RKIP-transgenic heart-rep1

RKIP-transgenic heart

strain background: B6 genotype/variation: RKIP-transgenic (expressing RKIP under control of the myocardium-specific alpha-MHC promoter) gender: male age: 2 months tissue: heart

Gene expression data of RKIP-transgenic heart RKIP-1

Sample_geo_accession	GSM1049430
Sample_status	Public on Dec 06 2012
Sample_submission_date	Dec 05 2012
Sample_last_update_date	Dec 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from a transgenic mouse line expressing RKIP under control of the myocardium-specific alpha-MHC promoter. For comparison, a transgenic mouse line with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) was used. The third group consisted of B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual;and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049430/suppl/GSM1049430_RKIP-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049430/suppl/GSM1049430_RKIP-1.CHP.gz
Sample_series_id	GSE42753
Sample_data_row_count	45101

GSM1049431

GPL1261

RKIP-transgenic heart-rep2

RKIP-transgenic heart

strain background: B6 genotype/variation: RKIP-transgenic (expressing RKIP under control of the myocardium-specific alpha-MHC promoter) gender: male age: 2 months tissue: heart

Gene expression data of RKIP-transgenic heart RKIP-2

Sample_geo_accession	GSM1049431
Sample_status	Public on Dec 06 2012
Sample_submission_date	Dec 05 2012
Sample_last_update_date	Dec 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from a transgenic mouse line expressing RKIP under control of the myocardium-specific alpha-MHC promoter. For comparison, a transgenic mouse line with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) was used. The third group consisted of B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual;and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049431/suppl/GSM1049431_RKIP-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049431/suppl/GSM1049431_RKIP-2.CHP.gz
Sample_series_id	GSE42753
Sample_data_row_count	45101

GSM1049432

GPL1261

GRK-Inh-transgenic heart-rep1

GRK-Inh-transgenic heart

strain background: B6 genotype/variation: GRK-Inh-transgenic (myocardium-specific expression of a GRK-specific peptide inhibitor) gender: male age: 2 months tissue: heart

Gene expression data of GRK-Inh-transgenic heart GRK-Inh-1

Sample_geo_accession	GSM1049432
Sample_status	Public on Dec 06 2012
Sample_submission_date	Dec 05 2012
Sample_last_update_date	Dec 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from a transgenic mouse line expressing RKIP under control of the myocardium-specific alpha-MHC promoter. For comparison, a transgenic mouse line with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) was used. The third group consisted of B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual;and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049432/suppl/GSM1049432_GRK-Inh-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049432/suppl/GSM1049432_GRK-Inh-1.CHP.gz
Sample_series_id	GSE42753
Sample_data_row_count	45101

GSM1049433

GPL1261

GRK-Inh-transgenic heart-rep2

GRK-Inh-transgenic heart

strain background: B6 genotype/variation: GRK-Inh-transgenic (myocardium-specific expression of a GRK-specific peptide inhibitor) gender: male age: 2 months tissue: heart

Gene expression data of GRK-Inh-transgenic heart GRK-Inh-2

Sample_geo_accession	GSM1049433
Sample_status	Public on Dec 06 2012
Sample_submission_date	Dec 05 2012
Sample_last_update_date	Dec 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from a transgenic mouse line expressing RKIP under control of the myocardium-specific alpha-MHC promoter. For comparison, a transgenic mouse line with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) was used. The third group consisted of B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual;and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049433/suppl/GSM1049433_GRK-Inh-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049433/suppl/GSM1049433_GRK-Inh-2.CHP.gz
Sample_series_id	GSE42753
Sample_data_row_count	45101

GSM1049434

GPL1261

B6 control heart-rep1

B6 control heart

strain background: B6 genotype/variation: wild type (control) gender: male age: 2 months tissue: heart

Gene expression data of B6 control heart B6-1

Sample_geo_accession	GSM1049434
Sample_status	Public on Dec 06 2012
Sample_submission_date	Dec 05 2012
Sample_last_update_date	Dec 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from a transgenic mouse line expressing RKIP under control of the myocardium-specific alpha-MHC promoter. For comparison, a transgenic mouse line with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) was used. The third group consisted of B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual;and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049434/suppl/GSM1049434_B6-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049434/suppl/GSM1049434_B6-1.CHP.gz
Sample_series_id	GSE42753
Sample_data_row_count	45101

GSM1049435

GPL1261

B6 control heart-rep2

B6 control heart

strain background: B6 genotype/variation: wild type (control) gender: male age: 2 months tissue: heart

Gene expression data of B6 control heart B6-2

Sample_geo_accession	GSM1049435
Sample_status	Public on Dec 06 2012
Sample_submission_date	Dec 05 2012
Sample_last_update_date	Dec 06 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from a transgenic mouse line expressing RKIP under control of the myocardium-specific alpha-MHC promoter. For comparison, a transgenic mouse line with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) was used. The third group consisted of B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual;and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049435/suppl/GSM1049435_B6-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049435/suppl/GSM1049435_B6-2.CHP.gz
Sample_series_id	GSE42753
Sample_data_row_count	45101

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