Search results for the GEO ID: GSE42771  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1049708 | GPL570 |
|
NOD.Scid mouse-expanded GRK2-expressing HEK clone-rep1
|
NOD.Scid mouse-expanded HEK clone with GRK2 expression
|
cell type: HEK cell clones
genotype/variation: GRK2
protocol: expanded for 4 weeks in NOD.Scid mice
|
Scid-GRK-1
GRK2-expressing HEK clone expanded for 4 weeks in immunodeficient NOD.Scid mice
Gene expression data of NOD.Scid mouse-expanded GRK2-expressing HEK clone
|
| Sample_geo_accession | GSM1049708
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049708/suppl/GSM1049708_Scid-GRK-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049708/suppl/GSM1049708_Scid-GRK-1.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049709 | GPL570 |
|
NOD.Scid mouse-expanded GRK2-expressing HEK clone-rep2
|
NOD.Scid mouse-expanded HEK clone with GRK2 expression
|
cell type: HEK cell clones
genotype/variation: GRK2
protocol: expanded for 4 weeks in NOD.Scid mice
|
Scid-GRK-2
GRK2-expressing HEK clone expanded for 4 weeks in immunodeficient NOD.Scid mice
Gene expression data of NOD.Scid mouse-expanded GRK2-expressing HEK clone
|
| Sample_geo_accession | GSM1049709
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049709/suppl/GSM1049709_Scid-GRK-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049709/suppl/GSM1049709_Scid-GRK-2.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049710 | GPL570 |
|
NOD.Scid mouse-expanded GRK2-K220R-expressing HEK clone-rep1
|
NOD.Scid mouse-expanded HEK clone with GRK2-K220R expression
|
cell type: HEK cell clones
genotype/variation: GRK2-K220R
protocol: expanded for 4 weeks in NOD.Scid mice
|
Scid-K220R-1
GRK2-K220R-expressing HEK clone expanded for 4 weeks in immunodeficient NOD.Scid mice
Gene expression data of NOD.Scid mouse-expanded GRK2-K220R-expressing HEK clone
|
| Sample_geo_accession | GSM1049710
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049710/suppl/GSM1049710_Scid-K220R-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049710/suppl/GSM1049710_Scid-K220R-1.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049711 | GPL570 |
|
NOD.Scid mouse-expanded GRK2-K220R-expressing HEK clone-rep2
|
NOD.Scid mouse-expanded HEK clone with GRK2-K220R expression
|
cell type: HEK cell clones
genotype/variation: GRK2-K220R
protocol: expanded for 4 weeks in NOD.Scid mice
|
Scid-K220R-2
GRK2-K220R-expressing HEK clone expanded for 4 weeks in immunodeficient NOD.Scid mice
Gene expression data of NOD.Scid mouse-expanded GRK2-K220R-expressing HEK clone
|
| Sample_geo_accession | GSM1049711
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049711/suppl/GSM1049711_Scid-K220R-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049711/suppl/GSM1049711_Scid-K220R-2.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049712 | GPL570 |
|
Cultured GRK2-expressing HEK cell clone-rep1
|
Cultured HEK cell clone with GRK2 expression
|
cell type: HEK cell clones
genotype/variation: GRK2
protocol: cultured in vitro
|
HEK-GRK-1
Cultured GRK2-expressing HEK cell clone
Gene expression data of cultured GRK2-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049712
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049712/suppl/GSM1049712_HEK-GRK-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049712/suppl/GSM1049712_HEK-GRK-1.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049713 | GPL570 |
|
Cultured GRK2-expressing HEK cell clone-rep2
|
Cultured HEK cell clone with GRK2 expression
|
cell type: HEK cell clones
genotype/variation: GRK2
protocol: cultured in vitro
|
HEK-GRK-2
Cultured GRK2-expressing HEK cell clone
Gene expression data of cultured GRK2-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049713
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049713/suppl/GSM1049713_HEK-GRK-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049713/suppl/GSM1049713_HEK-GRK-2.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049714 | GPL570 |
|
Cultured GRK2-K220R-expressing HEK cell clone-rep1
|
Cultured HEK cell clone with GRK2-K220R expression
|
cell type: HEK cell clones
genotype/variation: GRK2-K220R
protocol: cultured in vitro
|
HEK-K220R-1
Cultured GRK2-K220R-expressing HEK cell clone
Gene expression data of cultured GRK2-K220R-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049714
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049714/suppl/GSM1049714_HEK-K220R-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049714/suppl/GSM1049714_HEK-K220R-1.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049715 | GPL570 |
|
Cultured GRK2-K220R-expressing HEK cell clone-rep2
|
Cultured HEK cell clone with GRK2-K220R expression
|
cell type: HEK cell clones
genotype/variation: GRK2-K220R
protocol: cultured in vitro
|
HEK-K220R-2
Cultured GRK2-K220R-expressing HEK cell clone
Gene expression data of cultured GRK2-K220R-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049715
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049715/suppl/GSM1049715_HEK-K220R-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049715/suppl/GSM1049715_HEK-K220R-2.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049716 | GPL570 |
|
Re-cultured GRK2-expressing HEK cell clone after NOD.Scid mouse expansion-rep1
|
Re-cultured GRK2-expressing HEK cell clone after NOD.Scid mouse expansion
|
cell type: HEK cell clones
genotype/variation: GRK2
protocol: re-cultured in vitro after expansion in NOD.Scid mice
|
Ex-Scid-GRK-1
Re-cultured GRK2-expressing HEK cell clone after expansion in NOD.Scid mice
Gene expression data of re-cultured NOD.Scid-mouse-expanded GRK2-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049716
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049716/suppl/GSM1049716_Ex-Scid-GRK-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049716/suppl/GSM1049716_Ex-Scid-GRK-1.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049717 | GPL570 |
|
Re-cultured GRK2-expressing HEK cell clone after NOD.Scid mouse expansion-rep2
|
Re-cultured GRK2-expressing HEK cell clone after NOD.Scid mouse expansion
|
cell type: HEK cell clones
genotype/variation: GRK2
protocol: re-cultured in vitro after expansion in NOD.Scid mice
|
Ex-Scid-GRK-2
Re-cultured GRK2-expressing HEK cell clone after expansion in NOD.Scid mice
Gene expression data of re-cultured NOD.Scid-mouse-expanded GRK2-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049717
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049717/suppl/GSM1049717_Ex-Scid-GRK-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049717/suppl/GSM1049717_Ex-Scid-GRK-2.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049718 | GPL570 |
|
Re-cultured GRK2-K220R-expressing HEK cell clone after NOD.Scid mouse expansion-rep1
|
Re-cultured GRK2-K220R-expressing HEK cell clone after NOD.Scid mouse expansion
|
cell type: HEK cell clones
genotype/variation: GRK2-K220R
protocol: re-cultured in vitro after expansion in NOD.Scid mice
|
Ex-Scid-K220R-1
Re-cultured GRK2-K220R-expressing HEK cell clone after expansion in NOD.Scid mice
Gene expression data of re-cultured NOD.Scid-mouse-expanded GRK2-K220R-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049718
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049718/suppl/GSM1049718_Ex-Scid-K220R-1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049718/suppl/GSM1049718_Ex-Scid-K220R-1.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
|
GSM1049719 | GPL570 |
|
Re-cultured GRK2-K220R-expressing HEK cell clone after NOD.Scid mouse expansion-rep2
|
Re-cultured GRK2-K220R-expressing HEK cell clone after NOD.Scid mouse expansion
|
cell type: HEK cell clones
genotype/variation: GRK2-K220R
protocol: re-cultured in vitro after expansion in NOD.Scid mice
|
Ex-Scid-K220R-2
Re-cultured GRK2-K220R-expressing HEK cell clone after expansion in NOD.Scid mice
Gene expression data of re-cultured NOD.Scid-mouse-expanded GRK2-K220R-expressing HEK cell clone
|
| Sample_geo_accession | GSM1049719
| | Sample_status | Public on Dec 06 2012
| | Sample_submission_date | Dec 06 2012
| | Sample_last_update_date | Dec 06 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For the microarray study, cell pellets were isolated from three study groups: (i) NOD.Scid mouse-expanded HEK clones expressing either GRK2 or kinase-deficient GRK2-K220R (Scid), (ii) in vitro cultured HEK cell clones expressing GRK2 or GRK2-K220R (HEK), and (iii) in vitro re-cultured HEK cell clones after NOD.Scid mouse expansion (Ex-Scid).
| | Sample_growth_protocol_ch1 | The study was performed with HEK cell clones stably expressing GRK2 or the kinase-deficient GRK2-K220R mutant. HEK cell clones were either expanded for 4 weeks in vivo in immunodeficient NOD.Scid mice (3 months of age), cultured in vitro, or re-cultured in vitro after expansion in NOD.Scid mice
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated from HEK cell clones with the RNeasy Mini kit according to the manufacturer`s instructions (Qiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual).
| | Sample_hyb_protocol | For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Affymetrix Human genome U133 Plus 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
| | Sample_data_processing | The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 200.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Ursula,,Quitterer
| | Sample_contact_email | ursula.quitterer@pharma.ethz.ch
| | Sample_contact_phone | 0041 44 635 6001
| | Sample_contact_fax | 0041 44 635 6881
| | Sample_contact_department | Molecular Pharmacology
| | Sample_contact_institute | Swiss Federal Institute of Technology
| | Sample_contact_address | Winterthurerstrasse 190
| | Sample_contact_city | Zurich
| | Sample_contact_zip/postal_code | CH-8057
| | Sample_contact_country | Switzerland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049719/suppl/GSM1049719_Ex-Scid-K220R-2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1049nnn/GSM1049719/suppl/GSM1049719_Ex-Scid-K220R-2.CHP.gz
| | Sample_series_id | GSE42771
| | Sample_data_row_count | 54675
| | |
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