Search results for the GEO ID: GSE42802 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1050220 | GPL570 |
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293T_unstim
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293T, unstim
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tissue: embryonic kidney cells
cell line: 293T
treatment: unztim
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Sample_geo_accession | GSM1050220
| Sample_status | Public on Dec 08 2012
| Sample_submission_date | Dec 07 2012
| Sample_last_update_date | Dec 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were stimulated with recombinant IFNbeta for 12 hours and then lysed in Qiagen RLT buffer.
| Sample_growth_protocol_ch1 | Cells were cultured in plates at 37C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's protocols.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (IVT Express Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array in an Affymetrix oven (HO640), while rotating at 60rpm. GeneChips were washed and stained in the Affymetrix Fluidic 450 according to manufacturing protocols (using Affymetrix Script FS_0001).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS300.
| Sample_data_processing | The data were analyzed with RMA quantile normalization using GenePattern software.
| Sample_platform_id | GPL570
| Sample_contact_name | Mark,,Lee
| Sample_contact_department | MIT
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1050nnn/GSM1050220/suppl/GSM1050220_1-.CEL.gz
| Sample_series_id | GSE42802
| Sample_data_row_count | 54675
| |
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GSM1050221 | GPL570 |
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293T_IFNbeta_12hr
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293T, IFNbeta, 12hr
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tissue: embryonic kidney cells
cell line: 293T
treatment: IFNbeta, 12hr
|
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Sample_geo_accession | GSM1050221
| Sample_status | Public on Dec 08 2012
| Sample_submission_date | Dec 07 2012
| Sample_last_update_date | Dec 08 2012
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were stimulated with recombinant IFNbeta for 12 hours and then lysed in Qiagen RLT buffer.
| Sample_growth_protocol_ch1 | Cells were cultured in plates at 37C
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's protocols.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (IVT Express Kit, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array in an Affymetrix oven (HO640), while rotating at 60rpm. GeneChips were washed and stained in the Affymetrix Fluidic 450 according to manufacturing protocols (using Affymetrix Script FS_0001).
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS300.
| Sample_data_processing | The data were analyzed with RMA quantile normalization using GenePattern software.
| Sample_platform_id | GPL570
| Sample_contact_name | Mark,,Lee
| Sample_contact_department | MIT
| Sample_contact_institute | Broad Institute
| Sample_contact_address | 7 Cambridge Center
| Sample_contact_city | Cambridge
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02139
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1050nnn/GSM1050221/suppl/GSM1050221_2+.CEL.gz
| Sample_series_id | GSE42802
| Sample_data_row_count | 54675
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