Search results for the GEO ID: GSE42883 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1052734 | GPL1261 |
|
ESC expressing Neurog1 at 24h, biological rep1
|
RNA from mouse ESC after 24h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 24h
|
Gene expression data from ESC after 24h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052734
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052734/suppl/GSM1052734_ESC24h-rep1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052734/suppl/GSM1052734_ESC24h-rep1.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052735 | GPL1261 |
|
ESC expressing Neurog1 at 24h, biological rep2
|
RNA from mouse ESC after 24h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 24h
|
Gene expression data from ESC after 24h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052735
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052735/suppl/GSM1052735_ESC24h-rep2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052735/suppl/GSM1052735_ESC24h-rep2.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052736 | GPL1261 |
|
ESC expressing Neurog1 at 24h, biological rep3
|
RNA from mouse ESC after 24h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 24h
|
Gene expression data from ESC after 24h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052736
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052736/suppl/GSM1052736_ESC24h-rep3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052736/suppl/GSM1052736_ESC24h-rep3.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052737 | GPL1261 |
|
ESC expressing Neurog1 at 48h, biological rep 1
|
RNA from mouse ESC after 48h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 48h
|
Gene expression data from ESC after 48h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052737
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052737/suppl/GSM1052737_ESC48h-rep1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052737/suppl/GSM1052737_ESC48h-rep1.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052738 | GPL1261 |
|
ESC expressing Neurog1 at 48h, biological rep 2
|
RNA from mouse ESC after 48h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 48h
|
Gene expression data from ESC after 48h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052738
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052738/suppl/GSM1052738_ESC48h-rep2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052738/suppl/GSM1052738_ESC48h-rep2.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052739 | GPL1261 |
|
ESC expressing Neurog1 at 48h, biological rep 3
|
RNA from mouse ESC after 48h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 48h
|
Gene expression data from ESC after 48h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052739
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052739/suppl/GSM1052739_ESC48h-rep3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052739/suppl/GSM1052739_ESC48h-rep3.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052740 | GPL1261 |
|
ESC expressing Neurog1 at 72h, biological rep 1
|
RNA from mouse ESC after 72h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 72h
|
Gene expression data from ESC after 72h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052740
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052740/suppl/GSM1052740_ESC72h-rep1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052740/suppl/GSM1052740_ESC72h-rep1.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052741 | GPL1261 |
|
ESC expressing Neurog1 at 72h, biological rep 2
|
RNA from mouse ESC after 72h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 72h
|
Gene expression data from ESC after 72h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052741
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052741/suppl/GSM1052741_ESC72h-rep2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052741/suppl/GSM1052741_ESC72h-rep2.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052742 | GPL1261 |
|
ESC expressing Neurog1 at 72h, biological rep 3
|
RNA from mouse ESC after 72h transgene induction
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 72h
|
Gene expression data from ESC after 72h of transgene induction vs uninduced ESC
|
Sample_geo_accession | GSM1052742
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052742/suppl/GSM1052742_ESC72h-rep3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052742/suppl/GSM1052742_ESC72h-rep3.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052743 | GPL1261 |
|
ESC in maintenance culture, biological rep 1
|
RNA from mouse ESC in maintenance culture
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 0h
|
Gene expression data from uninduced ESC
|
Sample_geo_accession | GSM1052743
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052743/suppl/GSM1052743_ESCcontrol-rep1.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052743/suppl/GSM1052743_ESCcontrol-rep1.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
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GSM1052744 | GPL1261 |
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ESC in maintenance culture, biological rep 2
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RNA from mouse ESC in maintenance culture
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tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 0h
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Gene expression data from uninduced ESC
|
Sample_geo_accession | GSM1052744
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052744/suppl/GSM1052744_ESCcontrol-rep2.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052744/suppl/GSM1052744_ESCcontrol-rep2.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
|
GSM1052745 | GPL1261 |
|
ESC in maintenance culture, biological rep 3
|
RNA from mouse ESC in maintenance culture
|
tissue: ES Cells
genotype: 129P2/OlaHsd (E14, Ainv15)
age in vitro: 0h
|
Gene expression data from uninduced ESC
|
Sample_geo_accession | GSM1052745
| Sample_status | Public on Jan 15 2013
| Sample_submission_date | Dec 12 2012
| Sample_last_update_date | Jan 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Transgene induction in the presence (and absence, controls) of 1 ug/ml doxycycline, for 24, 48, 72h
| Sample_growth_protocol_ch1 | DMEM with 10% FBS, 10-4M beta-mercaptoethanol, 0.22 ug/ml l-glutamine, 1.33 ug/ml HEPES, 10000 U/ml hrLIF, 1.5ug/ml puromycin, 350 ug/ml G418
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA performed following manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA prepared following Affy protocols from 20 ug total RNA
| Sample_hyb_protocol | fragmented cRNA hybridized to Affymetrix mouse 430 2.0 arrays for 16h, 45oC
| Sample_scan_protocol | Array was washed and stained using an Affymetrix Gene Chip Fluidics Station 450, then scanned with an Affymetrix gene chip scanner 3000 7G with autoloader
| Sample_data_processing | Signal intensity was normalized by MAS 5.0.
| Sample_platform_id | GPL1261
| Sample_contact_name | K Sue,,OShea
| Sample_contact_email | oshea@umich.edu
| Sample_contact_phone | 734 763 2550
| Sample_contact_fax | 734 763 1166
| Sample_contact_department | Cell & Developmental Biology
| Sample_contact_institute | University of Michigan
| Sample_contact_address | 3051 BSRB, 109 Zina Pitcher Pl
| Sample_contact_city | Ann Arbor
| Sample_contact_state | MI
| Sample_contact_zip/postal_code | 48109-2200
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052745/suppl/GSM1052745_ESCcontrol-rep3.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1052nnn/GSM1052745/suppl/GSM1052745_ESCcontrol-rep3.CHP.gz
| Sample_series_id | GSE42883
| Sample_data_row_count | 45101
| |
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