Search results for the GEO ID: GSE42989  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1054562 | GPL570 |
|
293T + empty vector, biological rep1
|
293T + empty vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with empty control vector
|
| Sample_geo_accession | GSM1054562
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054562/suppl/GSM1054562_1A-293T+empty.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054563 | GPL570 |
|
293T + empty vector, biological rep2
|
293T + empty vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with empty control vector
|
| Sample_geo_accession | GSM1054563
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054563/suppl/GSM1054563_1C-293T+empty.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054564 | GPL570 |
|
293T + empty vector, biological rep3
|
293T + empty vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with empty control vector
|
| Sample_geo_accession | GSM1054564
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054564/suppl/GSM1054564_1D-293T+empty.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054565 | GPL570 |
|
293T + empty vector, biological rep4
|
293T + empty vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with empty control vector
|
| Sample_geo_accession | GSM1054565
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054565/suppl/GSM1054565_1E-293T+empty.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054566 | GPL570 |
|
293T + tRNA vector, biological rep1
|
293T + tRNA vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for human chr1.tRNA68-Gly(GCC) tRNA, which is cleaved to form mature CU1276
|
| Sample_geo_accession | GSM1054566
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054566/suppl/GSM1054566_2A-293T+tRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054567 | GPL570 |
|
293T + tRNA vector, biological rep2
|
293T + tRNA vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for human chr1.tRNA68-Gly(GCC) tRNA, which is cleaved to form mature CU1276
|
| Sample_geo_accession | GSM1054567
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054567/suppl/GSM1054567_2C-293T+tRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054568 | GPL570 |
|
293T + tRNA vector, biological rep3
|
293T + tRNA vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for human chr1.tRNA68-Gly(GCC) tRNA, which is cleaved to form mature CU1276
|
| Sample_geo_accession | GSM1054568
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054568/suppl/GSM1054568_2D-293T+tRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054569 | GPL570 |
|
293T + tRNA vector, biological rep4
|
293T + tRNA vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for human chr1.tRNA68-Gly(GCC) tRNA, which is cleaved to form mature CU1276
|
| Sample_geo_accession | GSM1054569
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054569/suppl/GSM1054569_2E-293T+tRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054570 | GPL570 |
|
293T + hairpin vector, biological rep1
|
293T + hairpin vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for artificially constructed CU1276 hairpin
|
| Sample_geo_accession | GSM1054570
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054570/suppl/GSM1054570_3A-293T+miRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054571 | GPL570 |
|
293T + hairpin vector, biological rep2
|
293T + hairpin vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for artificially constructed CU1276 hairpin
|
| Sample_geo_accession | GSM1054571
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054571/suppl/GSM1054571_3C-293T+miRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
|
GSM1054572 | GPL570 |
|
293T + hairpin vector, biological rep3
|
293T + hairpin vector
|
cell line: 293T
cell type: Human embryonic kidney
|
Gene expression from 293T transiently transfected with vector encoding for artificially constructed CU1276 hairpin
|
| Sample_geo_accession | GSM1054572
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054572/suppl/GSM1054572_3D-293T+miRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
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GSM1054573 | GPL570 |
|
293T + hairpin vector, biological rep4
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293T + hairpin vector
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cell line: 293T
cell type: Human embryonic kidney
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Gene expression from 293T transiently transfected with vector encoding for artificially constructed CU1276 hairpin
|
| Sample_geo_accession | GSM1054573
| | Sample_status | Public on Dec 19 2012
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | Dec 19 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were transiently transfected with plasmids using the PEI reagent, as described in EHRHARDT ET AL, SIGNAL TRANSDUCTION 2006; 6: 179-84
| | Sample_growth_protocol_ch1 | 293T were grown in 10cm plates with DMEM supplemented with 10% FBS and 1% penicillin/streptomycin according to standart tissue culture protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were collected in cold PBS 48 hours post-transfection, and pellets were lysed with Trizol reagent; RNA was purified according to manufacturers indications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized to Affymetrix U133p2 arrays according to manufacturer's indications
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Roy,Louis,Maute
| | Sample_contact_email | rlm2122@columbia.edu
| | Sample_contact_laboratory | Riccardo Dalla-Favera lab
| | Sample_contact_department | Institute for Cancer Genetics
| | Sample_contact_institute | Columbia University
| | Sample_contact_address | 1130 St. Nicholas Ave., ICRC 507
| | Sample_contact_city | New York
| | Sample_contact_state | NY
| | Sample_contact_zip/postal_code | 10032
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054573/suppl/GSM1054573_3E-293T+miRNA.CEL.gz
| | Sample_series_id | GSE42989
| | Sample_data_row_count | 54675
| | |
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