Search results for the GEO ID: GSE42997  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1054629 | GPL1261 |
|
WT_AT_1
|
Granulosa cells pooled from 5 WT mice treated with PMSG followed by hCG and collected at 4h post-hCG
|
cell type: granulosa
genotype/variation: wild type
|
4h after treatment
|
| Sample_geo_accession | GSM1054629
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054629/suppl/GSM1054629_Affy_2595_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054630 | GPL1261 |
|
WT_AT_2
|
Granulosa cells pooled from 5 WT mice treated with PMSG followed by hCG and collected at 4h post-hCG
|
cell type: granulosa
genotype/variation: wild type
|
4h after treatment
|
| Sample_geo_accession | GSM1054630
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054630/suppl/GSM1054630_Affy_2890_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054631 | GPL1261 |
|
WT_AT_3
|
Granulosa cells pooled from 5 WT mice treated with PMSG followed by hCG and collected at 4h post-hCG
|
cell type: granulosa
genotype/variation: wild type
|
4h after treatment
|
| Sample_geo_accession | GSM1054631
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054631/suppl/GSM1054631_Affy_2894_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054632 | GPL1261 |
|
WT_BT_1
|
Granulosa cells pooled from 5 WT mice treated with PMSG
|
cell type: granulosa
genotype/variation: wild type
|
before treatment
|
| Sample_geo_accession | GSM1054632
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054632/suppl/GSM1054632_Affy_2684_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054633 | GPL1261 |
|
WT_BT_2
|
Granulosa cells pooled from 5 WT mice treated with PMSG
|
cell type: granulosa
genotype/variation: wild type
|
before treatment
|
| Sample_geo_accession | GSM1054633
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054633/suppl/GSM1054633_Affy_2888_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054634 | GPL1261 |
|
WT_BT_3
|
Granulosa cells pooled from 5 WT mice treated with PMSG
|
cell type: granulosa
genotype/variation: wild type
|
before treatment
|
| Sample_geo_accession | GSM1054634
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054634/suppl/GSM1054634_Affy_2892_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054635 | GPL1261 |
|
EX6DEL_BT_1
|
Granulosa cells pooled from 5 Snfl2 Ex6DEL mice treated with PMSG
|
cell type: granulosa
genotype/variation: Snfl2 Ex6DEL
|
before treatment
|
| Sample_geo_accession | GSM1054635
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054635/suppl/GSM1054635_Affy_2683_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054636 | GPL1261 |
|
EX6DEL_BT_2
|
Granulosa cells pooled from 5 Snfl2 Ex6DEL mice treated with PMSG
|
cell type: granulosa
genotype/variation: Snfl2 Ex6DEL
|
before treatment
|
| Sample_geo_accession | GSM1054636
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054636/suppl/GSM1054636_Affy_2889_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054637 | GPL1261 |
|
EX6DEL_BT_3
|
Granulosa cells pooled from 5 Snfl2 Ex6DEL mice treated with PMSG
|
cell type: granulosa
genotype/variation: Snfl2 Ex6DEL
|
before treatment
|
| Sample_geo_accession | GSM1054637
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054637/suppl/GSM1054637_Affy_2893_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054638 | GPL1261 |
|
EX6DEL_AT_1
|
Granulosa cells pooled from 5 Snfl2 Ex6DEL mice treated with PMSG followed by hCG and collected at 4h post-hCG
|
cell type: granulosa
genotype/variation: Snfl2 Ex6DEL
|
4h after treatment
|
| Sample_geo_accession | GSM1054638
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054638/suppl/GSM1054638_Affy_2596_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054639 | GPL1261 |
|
EX6DEL_AT_2
|
Granulosa cells pooled from 5 Snfl2 Ex6DEL mice treated with PMSG followed by hCG and collected at 4h post-hCG
|
cell type: granulosa
genotype/variation: Snfl2 Ex6DEL
|
4h after treatment
|
| Sample_geo_accession | GSM1054639
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054639/suppl/GSM1054639_Affy_2891_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
|
GSM1054640 | GPL1261 |
|
EX6DEL_AT_3
|
Granulosa cells pooled from 5 Snfl2 Ex6DEL mice treated with PMSG followed by hCG and collected at 4h post-hCG
|
cell type: granulosa
genotype/variation: Snfl2 Ex6DEL
|
4h after treatment
|
| Sample_geo_accession | GSM1054640
| | Sample_status | Public on May 01 2013
| | Sample_submission_date | Dec 18 2012
| | Sample_last_update_date | May 02 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Female mice were injected intra-peritoneally (ip) at 24-26 days of age with 5 IU pregnant mares' serum gonadotropin (PMSG; Folligon; Intervet, Boxmeer, The Netherlands) to stimulate follicle growth. For the PMSG only treated group, granulosa cells were collected after 48h in both WT and Ex6DEL littermates (5 mice per group). For the PMSG and hCG treated, the PMSG injection was followed 48h later by a single intra-peritoneal (ip) injection of 5 IU human chorionic gonadotropin (hCG; Sigma, St. Louis, MO) and granulosa cells were collected after 4h in both WT and Ex6DEL mice (5 mice per group).
| | Sample_growth_protocol_ch1 | The Snf2l Ex6DEL transgenic mouse line and the appropriate wild-type (WT) littermate control mice were generously provided by David J. Picketts (Ottawa Hospital Research Institute, Ottawa, Ontario, Canada). The mice are a hybrid strain resulting from the cross between the ubiquitously expressing Gata-1 Cre transgenic line in a CD-1 background provided by Dr. S. Orkin (Howard Hughes Medical Institute, Chevy Chase MD, USA) and mice with loxP-flanked Snf2l exon 6 developed by Dr. D.J. Picketts in a 129Sv background. The resulting Ex6DEL line was bred to the germline and maintained independently of CRE on a 129SV background. Granulosa cells were harvested by puncturing individual follicles using a 25G needle and applying light pressure with tweezers.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Whole transcript sense target labeling assay
| | Sample_hyb_protocol | Affymetrix standard
| | Sample_scan_protocol | Affymetrix standard
| | Sample_data_processing | The data were analyzed with GCRMA as background correction, summarization & normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Carol,,Perez-Iratxeta
| | Sample_contact_email | cpereziratxeta@gmail.com
| | Sample_contact_phone | (613) 737-8899 -73255
| | Sample_contact_fax | (613) 737-8023
| | Sample_contact_laboratory | Cellular and Molecular Medicine, Bioinformatics
| | Sample_contact_department | Ottawa Health Research Institute
| | Sample_contact_institute | Ontario Genomics Innovation Centre (OGIC)
| | Sample_contact_address | 501 Smyth Rd.
| | Sample_contact_city | Ottawa
| | Sample_contact_state | ON
| | Sample_contact_zip/postal_code | K1H 8L6
| | Sample_contact_country | Canada
| | Sample_contact_web_link | http://www.ohri.ca
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1054nnn/GSM1054640/suppl/GSM1054640_Affy_2895_2.0.CEL.gz
| | Sample_series_id | GSE42997
| | Sample_data_row_count | 45101
| | |
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