Result

Search results for the GEO ID: GSE43042

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1055563

GPL1261

Ldb1+/+, biological rep1

hemangioblast equivalent cells

cell-type: Ldb1+/+ from Flk1+ cells genotype: Ldb1+/+ age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies

Gene expression data from hemangioblast in vitro equivalent cells with Ldb1 +/+.

Sample_geo_accession	GSM1055563
Sample_status	Public on Feb 09 2013
Sample_submission_date	Dec 19 2012
Sample_last_update_date	Feb 09 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin-labelled cRNA
Sample_label_protocol_ch1	RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
Sample_hyb_protocol	Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
Sample_data_processing	Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
Sample_platform_id	GPL1261
Sample_contact_name	supat,,thongjuea
Sample_contact_email	supat.thongjuea@bccs.uib.no
Sample_contact_phone	+47 55584722
Sample_contact_department	Bergen Center for Computational Science
Sample_contact_institute	UniComputing, UniResearch
Sample_contact_address	Høyteknologisenteret,Thormøhlensgate 55
Sample_contact_city	Bergen
Sample_contact_zip/postal_code	N-5008
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1055nnn/GSM1055563/suppl/GSM1055563_WT_B_Flk1+.CEL.gz
Sample_series_id	GSE43042
Sample_series_id	GSE43044
Sample_data_row_count	45101

GSM1055564

GPL1261

Ldb1+/+, biological rep2

hemangioblast equivalent cells

cell-type: Ldb1+/+ from Flk1+ cells genotype: Ldb1+/+ age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies

Gene expression data from hemangioblast in vitro equivalent cells with Ldb1 +/+.

Sample_geo_accession	GSM1055564
Sample_status	Public on Feb 09 2013
Sample_submission_date	Dec 19 2012
Sample_last_update_date	Feb 09 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin-labelled cRNA
Sample_label_protocol_ch1	RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
Sample_hyb_protocol	Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
Sample_data_processing	Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
Sample_platform_id	GPL1261
Sample_contact_name	supat,,thongjuea
Sample_contact_email	supat.thongjuea@bccs.uib.no
Sample_contact_phone	+47 55584722
Sample_contact_department	Bergen Center for Computational Science
Sample_contact_institute	UniComputing, UniResearch
Sample_contact_address	Høyteknologisenteret,Thormøhlensgate 55
Sample_contact_city	Bergen
Sample_contact_zip/postal_code	N-5008
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1055nnn/GSM1055564/suppl/GSM1055564_WT_C_Flk1+.CEL.gz
Sample_series_id	GSE43042
Sample_series_id	GSE43044
Sample_data_row_count	45101

GSM1055565

GPL1261

Ldb1+/+, biological rep3

hemangioblast equivalent cells

cell-type: Ldb1+/+ from Flk1+ cells genotype: Ldb1+/+ age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies

Gene expression data from hemangioblast in vitro equivalent cells with Ldb1 +/+.

Sample_geo_accession	GSM1055565
Sample_status	Public on Feb 09 2013
Sample_submission_date	Dec 19 2012
Sample_last_update_date	Feb 09 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin-labelled cRNA
Sample_label_protocol_ch1	RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
Sample_hyb_protocol	Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
Sample_data_processing	Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
Sample_platform_id	GPL1261
Sample_contact_name	supat,,thongjuea
Sample_contact_email	supat.thongjuea@bccs.uib.no
Sample_contact_phone	+47 55584722
Sample_contact_department	Bergen Center for Computational Science
Sample_contact_institute	UniComputing, UniResearch
Sample_contact_address	Høyteknologisenteret,Thormøhlensgate 55
Sample_contact_city	Bergen
Sample_contact_zip/postal_code	N-5008
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1055nnn/GSM1055565/suppl/GSM1055565_WT_E_Flk1+.CEL.gz
Sample_series_id	GSE43042
Sample_series_id	GSE43044
Sample_data_row_count	45101

GSM1055566

GPL1261

Ldb1-/-, biological rep1

hemangioblast equivalent cells

cell-type: Ldb1-/- from Flk1+ cells genotype: Ldb1-/- age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies

Gene expression data from hemangioblast in vitro equivalent cells without Ldb1.

Sample_geo_accession	GSM1055566
Sample_status	Public on Feb 09 2013
Sample_submission_date	Dec 19 2012
Sample_last_update_date	Feb 09 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin-labelled cRNA
Sample_label_protocol_ch1	RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
Sample_hyb_protocol	Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
Sample_data_processing	Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
Sample_platform_id	GPL1261
Sample_contact_name	supat,,thongjuea
Sample_contact_email	supat.thongjuea@bccs.uib.no
Sample_contact_phone	+47 55584722
Sample_contact_department	Bergen Center for Computational Science
Sample_contact_institute	UniComputing, UniResearch
Sample_contact_address	Høyteknologisenteret,Thormøhlensgate 55
Sample_contact_city	Bergen
Sample_contact_zip/postal_code	N-5008
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1055nnn/GSM1055566/suppl/GSM1055566_KO_B_Flk1+.CEL.gz
Sample_series_id	GSE43042
Sample_series_id	GSE43044
Sample_data_row_count	45101

GSM1055567

GPL1261

Ldb1-/-, biological rep2

hemangioblast equivalent cells

cell-type: Ldb1-/- from Flk1+ cells genotype: Ldb1-/- age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies

Gene expression data from hemangioblast in vitro equivalent cells without Ldb1.

Sample_geo_accession	GSM1055567
Sample_status	Public on Feb 09 2013
Sample_submission_date	Dec 19 2012
Sample_last_update_date	Feb 09 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin-labelled cRNA
Sample_label_protocol_ch1	RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
Sample_hyb_protocol	Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
Sample_data_processing	Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
Sample_platform_id	GPL1261
Sample_contact_name	supat,,thongjuea
Sample_contact_email	supat.thongjuea@bccs.uib.no
Sample_contact_phone	+47 55584722
Sample_contact_department	Bergen Center for Computational Science
Sample_contact_institute	UniComputing, UniResearch
Sample_contact_address	Høyteknologisenteret,Thormøhlensgate 55
Sample_contact_city	Bergen
Sample_contact_zip/postal_code	N-5008
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1055nnn/GSM1055567/suppl/GSM1055567_KO_C_Flk1+.CEL.gz
Sample_series_id	GSE43042
Sample_series_id	GSE43044
Sample_data_row_count	45101

GSM1055568

GPL1261

Ldb1-/-, biological rep3

hemangioblast equivalent cells

cell-type: Ldb1-/- from Flk1+ cells genotype: Ldb1-/- age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies

Gene expression data from hemangioblast in vitro equivalent cells without Ldb1.

Sample_geo_accession	GSM1055568
Sample_status	Public on Feb 09 2013
Sample_submission_date	Dec 19 2012
Sample_last_update_date	Feb 09 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin-labelled cRNA
Sample_label_protocol_ch1	RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
Sample_hyb_protocol	Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
Sample_data_processing	Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
Sample_platform_id	GPL1261
Sample_contact_name	supat,,thongjuea
Sample_contact_email	supat.thongjuea@bccs.uib.no
Sample_contact_phone	+47 55584722
Sample_contact_department	Bergen Center for Computational Science
Sample_contact_institute	UniComputing, UniResearch
Sample_contact_address	Høyteknologisenteret,Thormøhlensgate 55
Sample_contact_city	Bergen
Sample_contact_zip/postal_code	N-5008
Sample_contact_country	Norway
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1055nnn/GSM1055568/suppl/GSM1055568_KO_E_Flk1+.CEL.gz
Sample_series_id	GSE43042
Sample_series_id	GSE43044
Sample_data_row_count	45101

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