Search results for the GEO ID: GSE43416  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1062231 | GPL1261 |
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Hif-2αF/F
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Colon
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strain: C57BL/6
tissue: colon
genotype/variation: Hif-2αF/F
age: 6 to 8 weeks old
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Gene expression data from colons.
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| Sample_geo_accession | GSM1062231
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Jan 10 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Colon mucosal scrapings were got from 6 to 8 weeks old mice treated with 3% DSS for 3 days
| | Sample_growth_protocol_ch1 | All mice were C57BL/6 background and maintained in standard cages in a light and temperature-controlled room and were allowed standard chow and water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the Ambion Premier kit protocol from 250 ng total RNA (MessageAmp Premier RNA Amplification Kit Instruction Manual, 2008, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | XIANG,,XUE
| | Sample_contact_email | xxue@umich.edu
| | Sample_contact_phone | 7346155041
| | Sample_contact_institute | Umich
| | Sample_contact_address | 1301 E. Catherine Street
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | Michigan
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1062nnn/GSM1062231/suppl/GSM1062231_Shah_007_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE43416
| | Sample_data_row_count | 45101
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GSM1062232 | GPL1261 |
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Hif-2αΔIE
|
Colon
|
strain: C57BL/6
tissue: colon
genotype/variation: Hif-2αΔIE
age: 6 to 8 weeks old
|
Gene expression data from colons.
|
| Sample_geo_accession | GSM1062232
| | Sample_status | Public on Feb 28 2013
| | Sample_submission_date | Jan 10 2013
| | Sample_last_update_date | Feb 28 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Colon mucosal scrapings were got from 6 to 8 weeks old mice treated with 3% DSS for 3 days
| | Sample_growth_protocol_ch1 | All mice were C57BL/6 background and maintained in standard cages in a light and temperature-controlled room and were allowed standard chow and water ad libitum.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated aRNA were prepared according to the Ambion Premier kit protocol from 250 ng total RNA (MessageAmp Premier RNA Amplification Kit Instruction Manual, 2008, Ambion).
| | Sample_hyb_protocol | Following fragmentation, 16 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
| | Sample_data_processing | Data was analyzed using the affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | XIANG,,XUE
| | Sample_contact_email | xxue@umich.edu
| | Sample_contact_phone | 7346155041
| | Sample_contact_institute | Umich
| | Sample_contact_address | 1301 E. Catherine Street
| | Sample_contact_city | Ann Arbor
| | Sample_contact_state | Michigan
| | Sample_contact_zip/postal_code | 48109
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1062nnn/GSM1062232/suppl/GSM1062232_Shah_008_Mouse430_2_.CEL.gz
| | Sample_series_id | GSE43416
| | Sample_data_row_count | 45101
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