Search results for the GEO ID: GSE43608  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1066170 | GPL570 |
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HCT116 3 days normoxia exp A
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HCT116
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disease state: colon cancer
cell line: HCT116 colon cancer cells
treatment protocol: normoxia
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HCT116 3 days normoxia exp A.
|
| Sample_geo_accession | GSM1066170
| | Sample_status | Public on Jan 18 2013
| | Sample_submission_date | Jan 18 2013
| | Sample_last_update_date | Jan 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Serum starved HCT116 were placed under normoxia or hypoxia for 72 hours.
| | Sample_growth_protocol_ch1 | Subconfluent HCT116 were washed with PBS once and media without serum was added for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen extraction kits.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicole,,Fer
| | Sample_contact_email | fern@mail.nih.gov
| | Sample_contact_institute | Frederick National Laboratory for Cancer Research
| | Sample_contact_address | PO Box B
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066170/suppl/GSM1066170_Onnis_N3A_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066170/suppl/GSM1066170_Onnis_N3A_1.mas5.CHP.gz
| | Sample_series_id | GSE43608
| | Sample_data_row_count | 54675
| | |
|
GSM1066171 | GPL570 |
|
HCT116 3 days hypoxia exp A
|
HCT116
|
disease state: colon cancer
cell line: HCT116 colon cancer cells
treatment protocol: hypoxia
|
HCT116 3 days hypoxia exp A.
|
| Sample_geo_accession | GSM1066171
| | Sample_status | Public on Jan 18 2013
| | Sample_submission_date | Jan 18 2013
| | Sample_last_update_date | Jan 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Serum starved HCT116 were placed under normoxia or hypoxia for 72 hours.
| | Sample_growth_protocol_ch1 | Subconfluent HCT116 were washed with PBS once and media without serum was added for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen extraction kits.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicole,,Fer
| | Sample_contact_email | fern@mail.nih.gov
| | Sample_contact_institute | Frederick National Laboratory for Cancer Research
| | Sample_contact_address | PO Box B
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066171/suppl/GSM1066171_Onnis_H3A_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066171/suppl/GSM1066171_Onnis_H3A_1.mas5.CHP.gz
| | Sample_series_id | GSE43608
| | Sample_data_row_count | 54675
| | |
|
GSM1066172 | GPL570 |
|
HCT116 3 days normoxia exp B
|
HCT116
|
disease state: colon cancer
cell line: HCT116 colon cancer cells
treatment protocol: normoxia
|
HCT116 3 days normoxia exp B.
|
| Sample_geo_accession | GSM1066172
| | Sample_status | Public on Jan 18 2013
| | Sample_submission_date | Jan 18 2013
| | Sample_last_update_date | Jan 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Serum starved HCT116 were placed under normoxia or hypoxia for 72 hours.
| | Sample_growth_protocol_ch1 | Subconfluent HCT116 were washed with PBS once and media without serum was added for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen extraction kits.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicole,,Fer
| | Sample_contact_email | fern@mail.nih.gov
| | Sample_contact_institute | Frederick National Laboratory for Cancer Research
| | Sample_contact_address | PO Box B
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066172/suppl/GSM1066172_Onnis_N3B_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066172/suppl/GSM1066172_Onnis_N3B_1.mas5.CHP.gz
| | Sample_series_id | GSE43608
| | Sample_data_row_count | 54675
| | |
|
GSM1066173 | GPL570 |
|
HCT116 3 days hypoxia exp B
|
HCT116
|
disease state: colon cancer
cell line: HCT116 colon cancer cells
treatment protocol: hypoxia
|
HCT116 3 days hypoxia exp B.
|
| Sample_geo_accession | GSM1066173
| | Sample_status | Public on Jan 18 2013
| | Sample_submission_date | Jan 18 2013
| | Sample_last_update_date | Jan 18 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Serum starved HCT116 were placed under normoxia or hypoxia for 72 hours.
| | Sample_growth_protocol_ch1 | Subconfluent HCT116 were washed with PBS once and media without serum was added for 24 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using Qiagen extraction kits.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Nicole,,Fer
| | Sample_contact_email | fern@mail.nih.gov
| | Sample_contact_institute | Frederick National Laboratory for Cancer Research
| | Sample_contact_address | PO Box B
| | Sample_contact_city | Frederick
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 21702
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066173/suppl/GSM1066173_Onnis_H3B_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1066nnn/GSM1066173/suppl/GSM1066173_Onnis_H3B_1.mas5.CHP.gz
| | Sample_series_id | GSE43608
| | Sample_data_row_count | 54675
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