Search results for the GEO ID: GSE43651  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1067620 | GPL1261 |
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PP before Fusion
|
embryonic palate
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strain background: ICR
gender: female
developmental stage: fetus at E14
tissue: embryonic palate before fusion
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01_No.1
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| Sample_geo_accession | GSM1067620
| | Sample_status | Public on Jan 23 2013
| | Sample_submission_date | Jan 22 2013
| | Sample_last_update_date | Jan 23 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | For microarray analysis, the edge of the palatal processes was microdissected and collected using a microscope and forceps at the each stage of palatal development (before, during and after fusion) .
| | Sample_growth_protocol_ch1 | The maxillary portion was removed from each fetus at E14. Explants were grown with BGJB medium containing 1% penicillin/streptomycin and 95%O2/5%CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The extraction of total RNA was performed with RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (One-Cycle Target Labeling, Expression Analysis Technical Manual).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings with no scaling (scale factor set to 1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takayoshi ,,Sakai
| | Sample_contact_email | sakai@dent.osaka-u.ac.jp
| | Sample_contact_phone | 81-6-6879-2275
| | Sample_contact_fax | 81-6-6879-2279
| | Sample_contact_department | Oral-Facial Disorders
| | Sample_contact_institute | Osaka Univ Grad Sch Dent
| | Sample_contact_address | 1-8 Yamadaoka
| | Sample_contact_city | Suita
| | Sample_contact_state | Osaka
| | Sample_contact_zip/postal_code | 5650871
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067620/suppl/GSM1067620_01_No.1_No_680.CEL.gz
| | Sample_series_id | GSE43651
| | Sample_data_row_count | 45101
| | |
|
GSM1067621 | GPL1261 |
|
PP during Fusion
|
embryonic palate
|
strain background: ICR
gender: female
developmental stage: fetus at E14
tissue: embryonic palate during fusion
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02_No.2
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| Sample_geo_accession | GSM1067621
| | Sample_status | Public on Jan 23 2013
| | Sample_submission_date | Jan 22 2013
| | Sample_last_update_date | Jan 23 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | For microarray analysis, the edge of the palatal processes was microdissected and collected using a microscope and forceps at the each stage of palatal development (before, during and after fusion) .
| | Sample_growth_protocol_ch1 | The maxillary portion was removed from each fetus at E14. Explants were grown with BGJB medium containing 1% penicillin/streptomycin and 95%O2/5%CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The extraction of total RNA was performed with RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (One-Cycle Target Labeling, Expression Analysis Technical Manual).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings with no scaling (scale factor set to 1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takayoshi ,,Sakai
| | Sample_contact_email | sakai@dent.osaka-u.ac.jp
| | Sample_contact_phone | 81-6-6879-2275
| | Sample_contact_fax | 81-6-6879-2279
| | Sample_contact_department | Oral-Facial Disorders
| | Sample_contact_institute | Osaka Univ Grad Sch Dent
| | Sample_contact_address | 1-8 Yamadaoka
| | Sample_contact_city | Suita
| | Sample_contact_state | Osaka
| | Sample_contact_zip/postal_code | 5650871
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067621/suppl/GSM1067621_02_No.2_No_680.CEL.gz
| | Sample_series_id | GSE43651
| | Sample_data_row_count | 45101
| | |
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GSM1067622 | GPL1261 |
|
PP after Fusion
|
embryonic palate
|
strain background: ICR
gender: female
developmental stage: fetus at E14
tissue: embryonic palate after fusion
|
03_No.3
|
| Sample_geo_accession | GSM1067622
| | Sample_status | Public on Jan 23 2013
| | Sample_submission_date | Jan 22 2013
| | Sample_last_update_date | Jan 23 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | For microarray analysis, the edge of the palatal processes was microdissected and collected using a microscope and forceps at the each stage of palatal development (before, during and after fusion) .
| | Sample_growth_protocol_ch1 | The maxillary portion was removed from each fetus at E14. Explants were grown with BGJB medium containing 1% penicillin/streptomycin and 95%O2/5%CO2 at 37C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | The extraction of total RNA was performed with RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (One-Cycle Target Labeling, Expression Analysis Technical Manual).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings with no scaling (scale factor set to 1).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Takayoshi ,,Sakai
| | Sample_contact_email | sakai@dent.osaka-u.ac.jp
| | Sample_contact_phone | 81-6-6879-2275
| | Sample_contact_fax | 81-6-6879-2279
| | Sample_contact_department | Oral-Facial Disorders
| | Sample_contact_institute | Osaka Univ Grad Sch Dent
| | Sample_contact_address | 1-8 Yamadaoka
| | Sample_contact_city | Suita
| | Sample_contact_state | Osaka
| | Sample_contact_zip/postal_code | 5650871
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1067nnn/GSM1067622/suppl/GSM1067622_03_No.3_No_680.CEL.gz
| | Sample_series_id | GSE43651
| | Sample_data_row_count | 45101
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